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Shim, Lee, Kim, Lee, Hwang, and Lee: Falcarindiol from Angelica koreana Down-regulated IL-8 and Up-regulated IL-10 in Colon Epithelial Cells
Original Article

Natural Product Sciences 2017;23(2):103-107.

Published online: 20 January 2017

DOI: https://doi.org/10.20307/nps.2017.23.2.103

Falcarindiol from Angelica koreana Down-regulated IL-8 and Up-regulated IL-10 in Colon Epithelial Cells

Sun-Yup Shim1, 2, 3, Seul-gi Lee1, Mihye Kim1, Jin Woo Lee4, Bang Yeon Hwang4, Mina Lee1, 2, ∗

1College of Pharmacy, Sunchon National University, Suncheon 57922, Jeonnam, Republic of Korea

2Suncheon Research Center for Natural Medicines, Suncheon 57922, Jeonnam, Republic of Korea

3Research Institute of Life and Pharmaceutical Sciences, Suncheon 57922, Jeonnam, Republic of Korea

4College of Pharmacy, Chungbuk National University, Cheongju 28644, Republic of Korea

∗Author for correspondence Mina Lee, College of Pharmacy, Sunchon National University, Korea Tel: +82-61-750-3764; E-mail: minalee@sunchon.ac.kr

Received 16 June 2016       Revised 24 February 2017       Accepted 27 February 2017

Copyright © 2017 The Korean Society of Pharmacognosy

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Angelica koreana is an important medicinal plant for some locals in East Asia including Korea. A few reports have shown the efficacy of its phytochemical constituents. We have isolated and purified one compound falcarindiol (FAL) from the methanolic extract of A. koreana roots. At concentrations from to 1 µM to 25 µM, the FAL isolated from the roots of A. koreana exerted no significant cytotoxicity and down-regulated LPS-stimulated pro-inflammatory cytokine IL-8 in colon epithelial cells, while up-regulating anti-inflammatory cytokine IL-10. In addition, the FAL decreased the expression of LPS-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 protein by Western blot analysis. Colon epithelial cells play pivotal roles in regulating the colon immune system and thus FAL is expected to be candidate agent as therapeutic potential for the treatment of inflammatory bowel disease (IBD) by modulating LPS-induced inflammation in colon epithelial cells.

Keywords: Angelica koreana, Falcarindiol, Inflammatory bowel disease, Colon epithelial cells

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Fig. 1.
The chemical structure of falcarindiol (FAL) isolated from A. koreana.
nps-23-103f1.tif
Fig. 2.
Effects of FAL on the cell viability of HT-29 colon epithelial cells. The cells were preincubated in the presence of the indicated concentrations of FAL for 1 h and then treated with LPS (1 µg/mL) for 24 h. Cell viability was assessed using the CCK-8 kit. The results are presented as the mean ± SD of three individual experiments.
nps-23-103f2.tif
Fig. 3.
Effects of FAL on IL-8 production in LPS-stimulated colon epithelial cells. The cells were preincubated for 1h with FAL, then incubated with LPS for 12 h. IL-8 levels were then determined by ELISA (mean ± SD, n=3). ## p <0.01, compared with untreated control; ∗p < 0.05 and ∗∗ p < 0.01, compared with LPS-treated control.
nps-23-103f3.tif
Fig. 4.
Effects of FAL on IL-10 production in LPS-stimulated colon epithelial cells. The cells were preincubated for 1 h with FAL, then incubated with LPS for 12 h. IL-10 levels were then determined by ELISA (mean ± SD, n=3). ∗∗∗ p <0.001, compared with LPS-treated control.
nps-23-103f4.tif
Fig. 5.
Effects of FAL on the expression of LPS-induced iNOS and COX-2 protein in HT-29 colon epithelial cells. Cells were pretreated with FAL at the indicated concentrations for 1 h and then treated with LPS (1 µg/mL) for 24 h. Cell lysates were prepared and the iNOS, COX-2 and actin protein levels were determined by western blotting. The relative intensity of iNOS and COX-2 to actin bands was measured by densitometry.
nps-23-103f5.tif
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