Induction of Apoptosis with Kigelia africana fruits in HCT116 Human Colon Cancer Cells via MAPKs Signaling Pathway

Tae-Eun Guon; Ha Sook Chung

doi:10.20307/nps.2016.22.3.209

Journal List > Nat Prod Sci > v.22(3) > 1060620

Go to TopGo to Top Go to BottomGo to Bottom

TOOLS

Guon and Chung: Induction of Apoptosis with Kigelia africana fruits in HCT116 Human Colon Cancer Cells via MAPKs Signaling Pathway

Original Article

Natural Product Sciences 2016;22(3):209-215.

Published online: 17 January 2016

DOI: https://doi.org/10.20307/nps.2016.22.3.209

Induction of Apoptosis with Kigelia africana fruits in HCT116 Human Colon Cancer Cells via MAPKs Signaling Pathway

Tae-Eun Guon¹, Ha Sook Chung^1,^∗

¹College of Natural Sciences, Duksung Women's University, Seoul 01369, Republic of Korea

^∗Author for correspondence Dr. Ha Sook Chung, College of Natural Sciences, Duksung Women's University, 144 Gil 33, Samyang-ro, Dobong-gu, Seoul 01369, Korea. Tel: +82-2-901-8593; E-mail: hasook@duksung.ac.kr

Received 3 December 2015 Revised 31 March 2016 Accepted 31 March 2016

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Kigelia africana (Lam.) Benth. (Bignoniaceae) is a flowering plants in South, Central and West Africa and commonly known as the sausage tree (Eng.); worsboom (Afr.); umVunguta, umFongothi (Zulu); Modukguhlu (North Sotho); Muvevha (Venda). The dried, powdered fruits are used as dressing for wounds and ulcers, haemorrhoids, rheumatism, purgative, skin-firming, lactation in breastfeeding mothers. The aim of this study is to investigate the cytotoxic and apoptotic potentials of 70% ethanolic extracts of Kigelia africana fruits in HCT116 human colon cancer cells. Treatment of Kigelia africana fruits with various concentrations resulted in a sequence of characteristic of apoptosis, including loss of cell viability and morphological changes. Flow cytometry analysis showed Kigelia africana fruits increased the sub-G1 phase (apoptosis) population. Apoptosis confirmed by annexin V-fluorescein isothiocyanate and propidium iodide double staining in HCT116 human colon cancer cell lines. Moreover, analysis of the mechanism indicated that Kigelia africana fruits showed an increased Bax and Bcl-2 expressions in a dose-dependent manner, resulting in activation of hallmarks of apoptotic events, caspase-3, caspase-9 and cleaved poly-ADP-ribose polymerase. This is the first report to demonstrate the cytotoxicity of Kigelia africana fruits on HCT116 human colon cancer cells.

Keywords: Cytotoxicity, MAPK signaling pathway, Hallmarks of apoptosis

REFERENCES

(1). Jemal A., Bray F., Center M. M., Ferlay J., Ward E., Forman D. CA. Cancer J. Clin. 2011; 61:69–90.

(2). Line-Edwige M., Raymond F. G., Francois E., François E., Edouard N. E. Afr. J.Tradit. Complement. Altern. Med. 2009; 6:112–117.

(3). Sun Q., Chen T., Wang X., Wei X. J.Cell Physiol. 2010; 222:421–432.

(4). Thornberry N. A., Rano T. A., Peterson E. P., Rasper D. M., Timkey T., Garcia-Calvo M., Houtzager V. M., Nordstrom P. A., Roy S., Vaillancourt J. P., Chapman K. T., Nicholson D. W. J.Biol. Chem. 1997; 272:17907–17911.

(5). Wang X.Genes Dev. 2001; 15:2922–2933.

(6). Reuter S., Eifes S., Dicato M., Aggarwal B. B., Diederich M.Biochem. Pharmacol. 2008; 76:1340–1351.

(7). Balaban R. S., Nemoto S., Finket T.Cell. 2005; 120:483–495.

(8). Madesh M., Antonsson B., Srinivasula S. M., Alnemri E. S., Hajnóczky G. J.Biol. Chem. 2002; 277:5651–5659.

(9). Fernandes-Alnemri T., Litwack G., Alnemri E. S. J.Biol. Chem. 1994; 269:30761–30764.

(10). Saini S., Kaur H., Verma B., Ripudaman Singh S. K.Nat. Prod. Rad. 2009; 8:190–197.

(11). Higgins C. A., Bell T., Delbederi Z., Feutren-Burton S., McClean B., O'Dowd C., Watters W., Armstrong P., Waugh D., van den Berg H.Planta Med. 2010; 76:1840–1846.

(12). Agyare C., Dwobeng A. S., Agyepong N., Boakye Y. D., Mensah K. B., Ayande P. G., Adarkwa-Yiadom M.See comment in PubMed Commons below Adv. Pharmacol. Sci. 2013; Article ID 692613. 10.

(13). Picerno P., Autore G., Marzocco S., Meloni M., Sanogo R., Aquino R. P. J.Nat. Prod. 2005; 68:1610–1614.

(14). Carmichael J., DeGraff W. G., Gazdar A. F., Minna J. D., Mitchell J. B.Cancer Res. 1987; 47:936–942.

(15). Lizard G., Fournel S., Genestier L., Dhedin N., Chaput C., Flacher M., Mutin M., Panaye G., Revillard J. P.Cytometry. 1995; 21:275–283.

(16). Nicoletti I., Migliorati G., Paqliacci M. C., Grignani F., Riccardi C. J.Immunol. Methods. 1991; 139:271–279.

(17). Ryu M. J., Kim A. D., Kang K. A., Chung H. S., Kim H. S., Suh I. S., Chang W. Y., Hyun J. W.In Vitro Cell. Dev. Biol. Anim. 2013; 49:74–81.

(18). Strickland L., Letson G. D., Muro-Cacho C. A.Cancer Control. 2001; 8:252–261.

(19). Ryu M. J., Chung H. S.In Vitro Cell. Dev. Biol. Anim. 2015; 51:92–101.

(20). Bold R. J., Termuhlen P. M., McConkey D. J.Surg. Oncol. 1997; 6:133–142.

(21). Xu Y., Ge R., Du J., Xin H., Yi T., Sheng J., Wang Y., Ling C.Cancer Lett. 2009; 284:229–237.

(22). Novák B., Tyson J. J.Biochem. Soc. Trans. 2003; 31:1526–1529.

(23). Han C. R., Jun D. Y., Woo H. J., Jeong S. Y., Woo M. H., Kim Y. H.Bioorg. Med. Chem. Lett. 2012; 22:945–953.

(24). Cory S., Adams J. M.Nat. Rev. Cancer. 2002; 2:647–656.

(25). Nagappan A., Park K. I., Park H. S., Kim J. A., Hong G. E., Kang S. R., Lee D. H., Kim E. H., Lee W. S., Won C. K., Kim G. S.Food Chem. 2012; 135:1920–1928.

(26). Kim K. N., Ham Y. M., Moon J. Y., Kim M. J., Jung Y. H., Jeon Y. J., Lee N. H., Kang N., Yang H. M., Kim D., Hyun C. G.Food Chem. 2012; 135:2112–2117.

(27). Oliver F. J., de la Rubia G., Rolli V., Ruiz-Ruiz M. C., de Murcia G., Murcia J. M. J.Biol. Chem. 1998; 273:33533–33539.

(28). Cuadrado A., Nebreda A. R.Biochem. J. 2010; 429:403–417.

(29). Ahmed-Choudhury J., Williams K. T., Young L. S., Adams D. H., Afford S. C.Cell Signal. 2006; 18:456–468.

Fig. 1.

Cytotoxic effect of KAF in (A) normal human colon cells and (B) human colon cancer cells line. Cell viability at the indicated concentrations of KAF in HT-29 and HCT116 cells at 72 hr was assessed by MTT assay. ∗p < 0.05, significantly different from control cells.

Fig. 2.

Induction of apoptosis by KAF in HCT116 human colon cancer cells. The formation of apoptotic bodies (arrows) in Hoechst-33258-stained cells observed by fluorescent microscopy.

Fig. 3.

Effects of apoptosis by KAF in HCT116 human colon cancer cells. (A) Flow cytometric analysis of HCT116 human colon cancer cells incubated with KAF for 72 h. The right bottom quadrant represents Annexin V-stained cells (early-phase apoptotic cells). The top right quadrant represents PI- and Annexin V-stained cells (late-phase apoptotic cells). (B) Statistical analysis of apoptosis. ∗p < 0.05, significantly different from control cells.

Fig. 4.

Effects of KAF on cell cycle phase distribution in HCT116 cells. After incubation with various concentrations of KAF for 72 hr, sub-G1 cells were detected by flow cytometry after propidium iodide stainning.∗p < 0.05, significantly different from control cells.

Fig. 5.

Effects of KAF on the expression of Bcl-2 family and caspase proteins in HCT116 cells. Cell lysates were electrophoresed Bcl-2, Bax, caspase-3, caspase-8, caspase-9, and cleaved PARP were detected by Western blotting analysis with the corresponding antibodies.

Fig. 6.

Effects of KAF on the expression of MAPK in HCT116 cells. Cell lysates were electrophoresed and JNK, ERK, and p38 were detected by Western blotting analysis with the corresponding antibodies.

Fig. 7.

Role of ERK (A) and p38 (B) in KAF-induced apoptosis in HCT116 cells. The cells were stimulated with 0.8 mg/mL of KAF after pre-treatment with PD98059 and SB202190, respectively. Equal amounts of cell lysates were electrophoresed and MAPK-related protein expression form were detected by Western blotting analysis with corresponding antibodies.

TOOLS

Similar articles