Journal List > Nat Prod Sci > v.22(1) > 1060618

Puttarak, Brantner, and Panichayupakaranant: Biological Activities and Stability of a Standardized Pentacyclic Triterpene Enriched Centella asiatica Extract

Abstract

Pentacyclic triterpenes, mainly, asiatic acid, madecassic acid, asiaticoside, and madecassoside are the active constituents of Centella asiatica. A pentacyclic triterpene enriched C. asiatica extract (PRE) was prepared and standardized to contain a total pentacyclic triterpenes not less than 65% w/w. This work was focused on determination of antiinflammatory, antioxidant, and tyrosinase inhibitory activities of PRE and its stability. The PRE exhibited a satisfactory nitric oxide inhibitory effect, with an IC50 value of 64.6 µg/mL. In addition, the PRE inhibited tyrosinase enzyme activity with an IC50 value of 104.8 µg/mL. In contrast, the PRE possessed only weak antioxidant activity. The PRE was stable over a period of four months when stored as a dried powder but only in a well-closed container protected from light at 4oC. An aqueous alcoholic solution of the PRE was stable at pH values of 5.8 and 7.0, but was not stable at a pH of 8.2. Preparations of the PRE in an aqueous solution should be performed in acidic or neutral conditions.

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Fig. 1.
HPLC chromatogram of the standardized PRE. Madecassoside (1); asiaticoside (2); madecassic acid (3); asiatic acid (4).
nps-22-20f1.tif
Fig. 2.
Effect of light on the stability of the PRE.
nps-22-20f2.tif
Fig. 3.
Effect of temperature on the stability of the PRE.
nps-22-20f3.tif
Fig. 4.
Effect of accelerated conditions on the stability of PRE.
nps-22-20f4.tif
Fig. 5.
Effect of pH on the stability of PRE.
nps-22-20f5.tif
Table 1.
Biological activities of the PRE and four pure pentacyclic triterpenes from C. asiatica
Samples IC50 µg/mL (µM)
NO inhibition DPPH Lipid peroxidation Tyrosinase inhibition
PRE 64.6 348.3 n.a.# 104.8
Asiatic acid 12.4 (25.4) n.a. n.a.# n.a.#
Madecassic acid n.a.+ n.a. n.a.# n.a.#
Asiaticoside n.a.+ n.a. n.a.# n.a.#
madecassoside n.a.+ n.a. n.a.# n.a.#
Aspirin 12.9 (71.7)
CAPE 1.6 (5.6)
BHT 60.9 (276.3)
Qurcetin 1.7 (5.6) 1.7 (5.6)
Kojic acid 5.0 (35.2)

− = not performed

+ n.a. = not active at concentration of 100 µg/mL

n.a. = not active at concentration of 400 µg/mL

# n.a. = not active at concentration of 1000 µg/mL

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