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Cho, Lee, Jung, Kim, Cho, and Lee: Phytochemical Identification from Boehmeria nivea Leaves and Analysis of (–)-Loliolide by HPLC
Original Article

Natural Product Sciences 2016;22(2):134-139.

Published online: 17 December 2016

DOI: https://doi.org/10.20307/nps.2016.22.2.134

Phytochemical Identification from Boehmeria nivea Leaves and Analysis of (–)-Loliolide by HPLC

Sunghun Cho1, Dong Gu Lee1, Yong-Su Jung2, Ho Bang Kim3, Eun Ju Cho4, Sanghyun Lee1, ∗

1Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, Korea

2Yeong-Gwang Agricultural Technology Center, Yeonggwang 57031, Korea

3Life Sciences Research Institute, Biomedic Co., Ltd., Bucheon 14548, Korea

4Department of Food Science and Nutrition, Pusan National University, Busan 46241, Korea

∗Author for correspondence Sanghyun Lee, Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, Korea Tel: +82-31-670-4688; E-mail: slee@cau.ac.kr

Received 21 December 2015       Revised 29 January 2016       Accepted 30 January 2016

Copyright © 2016 The Korean Society of Pharmacognosy

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Phytochemicals were isolated from leaves of the fiber crop, ramie (Boehmeria nivea, Bn), using open column chromatography and medium pressure liquid chromatography. Their structures were identified as β-sitosterol, (−)-loliolide, rutin, and pyrimidinedione by MS,1 H-, and13 C-NMR spectroscopic analysis. Among them, (−)-loliolide was isolated for the first time from B. nivea. A content analysis of (−)-loliolide in B. nivea collected from different regions and harvest times was conducted by HPLC. The highest content of (−)-loliolide was found in Bn-23 harvested in September. These results will be helpful to use the plant which harvest in September as a high content phytochemical additive in food, health supplements, and medicinal products.

Keywords: Boehmeria nivea, Urticaceae, Phytochemical, Identification, Loliolide, NMR

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Fig. 1.
Structures of compounds 1–4 from B. nivea.
nps-22-134f1.tif
Fig. 2.
HPLC chromatograms of (−)-loliolide (A) and the MeOH extract of Bn-23 (B) and −90 (C) collected in September.
nps-22-134f2.tif
Table 1.
Collection areas for Bn
Sample Collection area
Bn-02 Seobang variety in Hansan, Seocheon
Bn-07 Taiwan variety in Duwon, Goheung
Bn-10 Ramie in Mangun, Muan
Bn-23 Local variety in Hakgyo, Hampyeong
Bn-38 Local variety in Seocheon-4
Bn-41 White Peel variety in Biin, Seocheon-2
Bn-67 Improved variety in Gwangju, Taiwan variety in Goheung
Bn-76 Natural cross of White Peel variety
Bn-90 Local variety in Baeksu, Yeonggwang
Table 2.
LOD and LOQ of (−)-loliolide
Compound Calibration equationa r2b Linear range (mg/mL) LOD (mg/mL) LOQ (mg/mL)
(−)-loliolide Y = 5557.1X − 103.46 0.9999 0.005–5 0.055 0.142

a Y = peak area, X = concentration of standard (mg/mL).

b r2 = correlation coefficient for three data points in the calibration curve.

Table 3.
The content of (−)-loliolide in MeOH extracts of Bn
Harvest time Bn-02 Bn-07 Bn-10 Bn-23 Bn-38 Bn-41 Bn-67 Bn-76 Bn-90
June 5.02 6.35 6.93 5.89 4.31 4.24 4.91 5.12 5.46
July 4.32 6.42 7.72 7.97 4.05 4.32 5.65 6.67 5.39
August 3.52 5.17 3.90 4.27 3.26 4.72 3.82 3.30 3.31
September 7.04 7.25 7.43 7.86 6.76 6.38 7.60 6.79 4.77
Total 19.95 25.19 25.98 25.99 18.38 19.66 21.98 21.88 18.93

Data is represented as the mean ± SD (n = 3) in mg/g.

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