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Lee, Hwang, Im, Jeong, and Na: Anti-inflammatory Effect of Dactyloquinone B and Cyclospongiaquinone-1 Mixture in RAW264.7 Macrophage and ICR Mice
Original Article

Natural Product Sciences 2015;21(4):268-272.

Published online: 17 January 2015

DOI: https://doi.org/10.20307/nps.2015.21.4.268

Anti-inflammatory Effect of Dactyloquinone B and Cyclospongiaquinone-1 Mixture in RAW264.7 Macrophage and ICR Mice

Dong-Sung Lee1, †, In Hyun Hwang2, †, Nam-Kyung Im3, Gil-Saeng Jeong3, ∗, MinKyun Na4, ∗

1 Department of Biomedical Chemistry, Konkuk University, Chungju 380-701, Korea

2 Department of Chemistry, University of Iowa, Iowa City, IA 52242, United States

3 College of Pharmacy, Keimyung University, Daegu 704-701, Korea

4 College of Pharmacy, Chungnam National University; Daejeon 305-764, Korea

∗Author for correspondence Professor Gil-Saeng Jeong, College of Pharmacy, Keimyung University, 1095 Dalgubeol-daero, Daegu 704-701, Republic of Korea Tel: +82-53-580-6649; E-mail: gsjeong@kmu.ac.kr

Professor MinKyun Na, College of Pharmacy, Chungnam National University, 99 Daehak-ro, Yuseong, Daejeon 305-764, Republic of Korea Tel: +82-42-821-5925; E-mail: mkna@cnu.ac.kr

† These authors contributed equally to this work.

      Revised 14 July 201512 August 2015       Accepted 13 August 2015

Copyright © 2015 The Korean Society of Pharmacognosy

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Sesquiterpene-quinone is a class of secondary metabolites frequently encountered from marine sponge. The present study was designed to examine the anti-inflammatory action of sponge-derived dactyloquinone B (DQB) and cyclospongiaquinone-1 (CSQ1) mixture using lipopolysaccharide (LPS)-induced inflammatory responses. We measured the production of nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) and expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein. TNF-α, IL-1β, and IL-6 production, which increased by treatment with LPS, were significantly inhibited by DQB and CSQ1 mixture. It also decreased the production of NO production, and iNOS and COX-2 expression. Furthermore, it reduced 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced ear edema of ICR mice. These results demonstrate that sesquiterpene-quinone, DQB and CSQ1 mixture, might serve as a chemical pipeline for the development of anti-inflammatory agent.

Keywords: Dactyloquinone B, Cyclospongiaquinone-1, Anti-inflammatory effect, Ear edema model

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Fig. 1.
Chemical structures of dactyloquinone B (DQB) and cyclospongiaquinone-1 (CSQ1).
nps-21-268f1.tif
Fig. 2.
Effect of DQB and CSQ1 mixture on cell viability or NO production in RAW 264.7 cells. Cells were plated and cultured for 24 h in the absence or presence of different concentration of DQB and CSQ1 mixture (A). Cells were treated with DQB and CSQ1 mixture and LPS (100 ng/ml) for 24 h (B). NO production was calculated in culture media by Griess reagent (B). Each bar represents means ± S.D. of three independent experiments. ∗p <0.05 compared to the group treated with LPS.
nps-21-268f2.tif
Fig. 3.
Effect of DQB and CSQ1 mixture on LPS-induced proinflammatory cytokines in RAW 264.7 cells. Cells were treated with various concentrations of DQB and CSQ1 mixture for 1 h and incubated with LPS during 24 h. Productions of TNF-α (A), IL-1β (B), and IL-6 (C) were measured in culture media by ELISA. Each bar represents means ± S.D. of three independent experiments. ∗p < 0.05 compared to the group treated with LPS.
nps-21-268f3.tif
Fig. 4.
Effect of DQB and CSQ1 mixture on LPS-induced iNOS and COX-2 expression in RAW 264.7 cells. Cells were treated with various concentrations of DQB and CSQ1 mixture for 1 h and incubated with LPS during 24 h. The expression levels of iNOS and COX-2 in cells were analyzed by Western blot. Each bar represents means ± S.D. of three independent experiments. ∗p < 0.05 compared to the group treated with LPS.
nps-21-268f4.tif
Fig. 5.
Effect of DQB and CSQ1 mixture on TPA-induced ear edema in ICR mouse. Mice were administrated with or without the various concentrations of DQB and CSQ1 mixture. Curcumin was used as a positive control. TPA (2 μg/ear) was dropped onto the surface of the ear. The mice were sacrificed 4 h after the application of TPA. An ear disc 9.0 mm diameter was punched out of each ear and weighted. Values are shown as means ± S.D. of n=5 mice. ∗ p < 0.05 compared to the group treated with TPA.
nps-21-268f5.tif
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