Journal List > Korean J Endocr Surg > v.4(1) > 1060231

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Chung, Song, Park, Clark, Noh, Oh, Choe, and Youn: Regulation of Sodium-iodine Symporter Expression by Retinoic Acid in Thyroid Cancer Cell Lines
Original Article

Korean Journal of Endocrine Surgery 2004; 4(1): 1-9.

Published online: 30 June 2004

DOI: https://doi.org/10.16956/kjes.2004.4.1.1

Regulation of Sodium-iodine Symporter Expression by Retinoic Acid in Thyroid Cancer Cell Lines

Ki-Wook Chung, M.D.1, Min Sun Song, B.S.2, Jin-Woo Park, M.D.3, Orlo H. Clark, M.D.4, Dong-Young Noh, M.D.5, Seung Keun Oh, M.D.5, Kuk Jin Choe, M.D.5, Yeo-Kyu Youn, M.D.2, 5

1Center for Breast Cancer, National Cancer Center, Korea.

2Clinical Research Institute, Seoul National University Hospital, Korea.

3Department of Surgery, Chungbuk National University College of Medicine, Korea.

4Department of Surgery, University of California, San Francisco, Mt. Zion Medical Center, San Francisco, USA.

5Department of Surgery, Seoul National University College of Medicine, Korea.

Correspondence: ykyoun@plaza.snu.ac.kr

      Accepted 23 September 2004

Copyright © 2004 Korean Association of Thyroid and Endocrine Surgeons; KATES

(open-access):

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

Response to radioiodine therapy for thyroid cancer is related to the loss of sodium-iodine symporter protein caused by dedifferentiation of thyroid cancer cells. So we aimed to study mRNA expression of CD97, dedifferentiation marker, and sodium-iodine symporter after retinoic acid treatment according to retinoids receptor status.

Methods

Thyroid cancer cell lines; ARO, FRO, NPA, TPO, and FTC133 were prepared. 5µM of all trans retinoic acid were administered to each cell lines and then expression of m RNA for retinoids receptors (RARα, RARβ, RARγ, RXRα, RXRβ, RXRγ), CD97, and Sodium-Iodine symporter by RT-PCR.

Results

RARs and RXRs were differently expressed in each cell line. After retinoic acid treatment, relative density of retinoic acid receptor mRNA were increased by time dependently in each cell line except TPO cell line. Expression of CD97 also was decreased in every cell lines (P<0.001). Retinoic acid increased expression of sodium-iodine symporter only in FTC133 cell line (P<0.001), and TSH or forskolin did not enhance NIS expression by retinoic acid. RARβ and RXRγ were expressed only in FTC 133cell line before treatment. Induction of sodium-iodine symporter by retinoids disappeared after RARβ specific antagonist LE135 or pan RXR antagonist PA452 administration.

Conclusion

Retinoic acid reduced expression of CD97 in five thyroid cancer cell lines. However, retinoic acid could restore sodium-iodine symporter expression in only FTC 133 cell line specifically containing RARβ and RXRγ. Restoration of sodium-iodine symporter expression by retinoic acid is related to RARβ and RXRγ expression.

Keywords: Retinoids receptors, Thyroid cancer cell line, Sodium-iodine symporter, RARβ

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