Journal List > Korean J Endocr Surg > v.12(2) > 1060080

TOOLS
Hong, Park, Kang, Lee, Kim, Park, Kang, Kim, Hong, and Kim: Detection of BRAFV600E Mutation using Peptide Nucleic Acid-Mediated PCR Clamping Method in Fine Needle Aspiration Cytology
Original Article

Korean Journal of Endocrine Surgery 2012; 12(2): 87-91.

Published online: 30 June 2012

DOI: https://doi.org/10.16956/kjes.2012.12.2.87

Detection of BRAFV600E Mutation using Peptide Nucleic Acid-Mediated PCR Clamping Method in Fine Needle Aspiration Cytology

Jong-Chul Hong, M.D.1, Heon-Soo Park, M.D.1, Myung-Koo Kang, M.D.1, Joon-Hyung Lee, M.D.2, Deok-Kyu Kim, M.D.3, Mi-Kyung Park, M.D.3, Do-Young Kang, M.D.4, Sung-Heun Kim, M.D.5, Sook-Hee Hong, M.D.6, Dae-Cheol Kim, M.D.6

1Department of Otolaryngology-Head and Neck Surgery, Dong-A University College of Medicine, Busan, Korea.

2Department of Radiology, Dong-A University College of Medicine, Busan, Korea.

3Department of Endocrinology, Dong-A University College of Medicine, Busan, Korea.

4Department of Nuclear Medicine, Dong-A University College of Medicine, Busan, Korea.

5Department of Surgery, Dong-A University College of Medicine, Busan, Korea.

6Department of Pathology, Dong-A University College of Medicine, Busan, Korea.

Correspondence: freehand@dau.ac.kr

Received 3 April 2012       Revised 11 May 2012       Accepted 14 May 2012

Copyright © 2012 Korean Association of Thyroid and Endocrine Surgeons; KATES

(open-access):

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

The aim of this study was to evaluate the diagnostic value of a peptide nucleic acid (PNA)-mediated PCR clamping method for the detection of BRAFV600E mutations in fine needle aspiration cytology (FNAC).

Methods

One hundred sixty four patients underwent FNAC to evaluate BRAFV600E mutations between April 2011 and November 2011. Among them, forty-two patients were diagnosed with papillary thyroid carcinoma in a permanent pathologic specimen. A PNA-mediated PCR clamping method and a Dual-Priming Oligonucleotide (DPO)-based Real-time PCR method were used to detect the BRAFV600E mutation. We compared the result of mutation between the two methods.

Results

A BRAF mutation was found in 31 samples created by the PNA-mediated PCR clamping method, and in 28 samples in the DPO-based Real-time PCR method. The rate of BRAF mutation was 73.8% in association with the PNA-mediated PCR clamping method, and 66.7% in association with the DPO-based Real-time PCR method. There was no statistical differences between the two methods (P>0.05).

Conclusion

The PNA-mediated PCR clamping method may be an alternative to the DPO-based Real-Time PCR method for detection of BRAF mutations in thyroid nodules.

Keywords: Thyroid cancer, BRAF mutation

TOOLS
Similar articles