Journal List > Korean J Urogenit Tract Infect Inflamm > v.9(2) > 1059959

Korean J Urogenit Tract Infect Inflamm. 2014 Oct;9(2):99-103. Korean.
Published online October 31, 2014.  https://doi.org/10.14777/kjutii.2014.9.2.99
Copyright © 2014, Korean Association of Urogenital Tract Infection and Inflammation. All rights reserved.
Evaluation of Clinical Sample for Accupower UU Real-Time PCR Kit
Seung Ki Min, Soon Ki Kim, Yoo Seok Kim, In-Chang Cho and Gyeong In Lee1
Department of Urology, National Police Hospital, Seoul, Korea.
1Department of Laboratory Medicine, National Police Hospital, Seoul, Korea.

Correspondence to: Gyeong In Lee. Department of Laboratory Medicine, National Police Hospital, 123, Songi-ro, Songpa-gu, Seoul 138-708, Korea. Tel: +82-2-3400-1334, Fax: +82-2-3400-1566 Email: gyeonginlee@hanmail.net
Received October 03, 2014; Revised October 22, 2014; Accepted October 22, 2014.

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Purpose

In recent years, various diagnostic methods, including culture, immunological detection, conventional polymerase chain reaction (PCR) based methods, and microarray experiment have been applied for detection of Mycoplasma genitalium and Ureaplasma urealyticum infection. We assayed results of real time PCR and culture of variable clinical samples and evaluated various diagnostic indexes for assessing the clinical usefulness of the Accupower UU Real-Time PCR Kit (Bioneer Corp.) for detection of U. urealyticum/parvum.

Materials and Methods

We surveyed 111 results of culture test and antibiotic sensitivity test of Ureaplasma spp. that were requested to the department of laboratory medicine, National Police Hospital from January to April 2011. The specimens of Ureaplasma spp. were collected from 97 uterine cervical swab samples, 13 urine samples, and one expressed prostate secretion sample. Real-time PCR and culture methods were performed using the Accupower UU Real-Time PCR Kit (Bioneer Corp.) and Mycoplasma IST2 Kit (BioMérieux).

Results

The real-time PCR results showed that 80 clinical specimens were infected with U. urealyticum/parvum. These results were compared with those confirmed by microbiological culture. Compared with the culture, the diagnostic indexes (sensitivity, specificity, positive predictive value, and negative predictive value) of Accupower UU Real-Time PCR were 88.6%, 38.8%, 48.8%, and 83.9%, and the concordance between the Accupower UU Real-Time PCR Kit and the microbiological culture method was 58.5%.

Conclusions

Accupower UU Real-Time PCR is a very valuable technique which can process analysis of a massive number of samples with high speed, high sensitivity and specificity, and a high detection rate, particularly for Ureaplasma spp.

Keywords: Real-time polymerase chain reaction; Ureaplasma urealyticum; Ureaplasma parvum; Culture

Tables


Table 1
Number of specimens positive for Ureaplasma spp.
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Table 2
Comparison of culture with real-time PCR for detection of Ureaplasma spp.
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Table 3
Comparison of culture with real-time PCR for detection of Ureaplasma spp. by specimen types
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Table 4
Diagnostic index of Accupower UU Real-Time PCR (%)
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Notes

No potential conflict of interest relevant to this article was reported.

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