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Hyun Jung Jin1, Moon-Gyung Yoon2, Young-Hee Nam3, June Hong Ahn1, Hae-Sim Park2, Jin Hong Chung1
Abstract
Purpose
This study investigated the differences in the profile of IgE-binding components between Dermatophagoides pteronyssinus (Dp) and Dermatophagoides farina (Df) in respiratory allergic patients sensitized to Dp/Df.
Methods
Eighteen patients with respiratory allergic diseases having higher levels of serum specific IgE to Df compared to those to Dp (>twice) were enrolled. IgE-immunoblot analysis using Dp and Df extracts were used to compare the IgE binding components. Study subjects were classified into 2 groups according to the results of IgE-immunoblot analysis: 6 subjects having IgE-binding components to group 1 and 2 allergens (group B) and 12 subjects not having them (group A).
Results
Group A subjects were older (47.92±8.51 vs. 35.50±11.10, P=0.039) and males were dominant (75% vs. 0% P=0.009). IgE-immunoblot analysis demonstrated that all the group B subjects had IgE bindings to 2 major components, 14 and 25 kDa, while group A subjects had IgE bindings to high-molecular weight components ranging from 60–98 kDa. The enzyme-linked immunosorbent assay inhibition test showed a significant inhibition with additions of Df, not with Dp in group B subjects. Serum specific IgE levels to Dp and Df were significantly higher in group B than in group A, while its ratio (Df to Dp) was significantly higher in group A. No differences were noted in clinical parameters, total IgE, or eosinophil cationic protein levels.
Figures and Tables
Fig. 1
Comparisons of serum specific IgE binding components by IgE immunoblot using Dermatophagoides pteronyssinus (Dp) and Dermatophagoides farinae (Df). Group A, the subjects not having IgE-binding components to groups 1 and 2 allergens; group B, the subjects having IgE-binding components to groups 1 and 2 allergens; a, Using Dp extracts; b, Using Df extracts; PC, positive control which were derived from the results from the patients with higher serum specific IgE levels to both Dp and Df; NC, negative controls.
Fig. 2
IgE enzyme-linked immunosorbent assay inhibition tests with serial additions of Dp, Df in sera from group A (A) and group B (B) subjects. Dp, Dermatophagoides pteronyssinus; Df, Dermatophagoides farina.
Table 1
Clinical characteristics of group A subjects
Group A, the subjects not having IgE-binding components to groups 1 and 2 allergens; Dp, Dermatophagoides pteronyssinus; Df, Dermatophagoides farina; ECP, eosinophil cationic protein; TEC, total eosinophil count; ITX, allergen immunotherapy; HDM, house dust mite; AR, allergic rhinitis; AC, allergic conjunctivitis; AICU, aspirin intolerant chronic urticaria; AD, atopic dermatitis; EB, eosinophilic bronchitis; AERD, Aspirin-exacerbated respiratory disease; CU, chronic urticaria; BA, bronchial asthma; OAS, oral allergic syndrome; NA, not available; (+), the subject who has the history of immunotherapy with house dust mites or food allergy; (-), the subject has the history of immunotherapy with house dust mites or food allergy.
Table 2
Clinical characteristics of group B subjects
Group B, the subjects having IgE-binding components to groups 1 and 2 allergens; Dp, Dermatophagoides pteronyssinus; Df, Dermatophagoides farinae; ECP, eosinophil cationic protein; TEC, total eosinophil count; ITX, allergen immunotherapy; HDM, house dust mite; AR, allergic rhinitis; BA, bronchial asthma, NA, not available; AD, atopic dermatitis; AC, allergic conjunctivitis; (+), the subject who has the history of immunotherapy with house dust mites or food allergy; (-), the subject has the history of immunotherapy with house dust mites or food allergy.
Table 3
Comparison of clinical features between the 2 groups
Values are presented as mean±standard deviation.
Group A, the subjects not having IgE-binding components to groups 1 and 2 allergens; group B, the subjects having IgE-binding components to groups 1 and 2 allergens; Dp, Dermatophagoides pteronyssinus; Df, Dermatophagoides farinae; ECP, eosinophil cationic protein; TEC, total eosinophil count.
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