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Abstract
Purpose
Dysregulated cysteinyl leukotriene (CysLT) synthesis is prominent in exercise-induced bronchoconstriction (EIB). Secreted phospholipase A2 (sPLA2) plays a key regulatory role in the biosynthesis of CysLTs. We previously found that serum leptin levels correlate with (EIB) in children with asthma. The aim of this study was to address the relationship between plasma sPLA2/leptin levels and EIB.
Methods
Sixty-seven prepubertal children between the ages of 6 and 10 years were included in the study. They were asthmatics with EIB (n=25), asthmatics without EIB (n=21), and healthy subjects (n=21). We measured the plasma sPLA2 and leptin levels. We also performed pulmonary function tests at baseline, after bronchodilator inhalation, and after exercise.
Results
The sPLA2 and leptin levels were significantly higher in asthmatics with EIB than in those without and control subjects. In addition, sPLA2 levels were significantly correlated with body mass index (Speraman correlation coefficient r=0.343, P=0.023) and leptin levels (partial correlation coefficient r=318, P=0.033). The maximum decrease in % forced expiratory volume in 1 second after exercise was significantly correlated with both PLA2 levels (r=0.301, P=0.041) and leptin levels (r=0.346, P=0.018).
Figures and Tables
Fig. 1
Levels of plasma secreted phospholipase A2 (sPLA2) of study subject. EIB, exercise-induced bronchoconstriction.
Fig. 2
Secreted phospholipase A2 (sPLA2) levels were significantly correlated with serum leptin levels in asthmatic subjects (n=46, r=0.318, P=0.033). r, partial correlation coefficient adjusted for body mass index.
Fig. 3
The maximum decreases in % forced expiratory volume in 1 second (FEV1) after exercise were significantly correlated with both phospholipase A2 levels (A: n=46, r=0.301, P=0.041) and leptin levels (B: n=46, r=0.346, P=0.018). r, partial correlation coefficient adjusted for body mass index.
Table 1
Characteristics of the study subjects
Values are presented as mean±standard deviation unless otherwise indicated.
EIB, exercise-induced bronchoconstriction; ICS, inhaled corticosteroid; NA, not applicable; FEV1, forced expiratory volume in 1 second; pred %, predicted %; FVC, forced vital capacity; PC20, provocative concentration of methacholine inducing a 20% fall in FEV1.
*Kruskal-Wallis test. †P<0.05 vs. asthmatics without EIB. ‡P<0.05 vs. healthy. §Chi-square test.
Table 2
Biomarkers in peripheral blood (PB), and fractional exhaled nitric oxide in study subjects
Table 3
Correlation coefficients in asthma subjects
Leptin, FeNO, total IgE, and ECP were log-transformed.
sPLA2, secreted phospholipase A2; FEV1, forced expiratory volume in 1 second; pred %, predicted %; FVC, forced vital capacity; PC20, provocative concentration of methacholine inducing a 20% fall in FEV1; FeNO, fractional exhaled nitric oxide; PB, peripheral blood; ECP, eosinophil cationic protein.
*P<0.05. r, partial correlation coefficient adjusted for body mass index.
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