Journal List > Allergy Asthma Respir Dis > v.3(2) > 1059084

Lee, Sun, and Lee: Biohazard surveillance of allergic contact dermatitis in genetically-modified Zoysia grasses using patch testing

Abstract

Purpose

Genetic modification technique is widely used in plants for improving crop yields and qualities, and reducing pesticide use. Zoysia japonica is one of the widely planted grasses for lawning. Pollenless herbicide-tolerant genetically modified (GM) grasses (JG21-MS1 and JG21-MS2) were developed under surveillance for possible biohazard. Grasses may cause allergic contact dermatitis with direct contact on human skin. Patch testing with allergens was adopted to compare the incidences of allergic contact dermatitis to GM grass leaves with those of wild-type grass.

Methods

Patch testing with controls and leaves of wild-type and GM grasses was performed in individuals with informed consent.

Results

Ninety-seven individuals (mean age, 32±8 years; 48% males) were enrolled. For nickel sulfate as a positive control, 38 subjects (39.2%) were positive. For GM grasses, 10 (10.3%) showed positive patch test results and 11 (11.3%), for wild-type grass, the proportions of which were similar among the tested grasses.

Conclusion

JG21-MS1 and JG21-MS2 are not more biohazardous than wild-type grass in the risk of allergic contact dermatitis.

Figures and Tables

Fig. 1

Patch testing with Finn chambers: an actual preparation. Nickel, Nickel sulfate as a positive control; NC, blanked as a negative control; WT, wild type of Zoysia japonica; 1, JG21 (genetically modified Z. japonica for herbicide tolerance); 2, JG21-MS2 (genetically modified Z. japonica for herbicide tolerance and male sterility); 3, JG21-MS1 (genetically modified Z. japonica for herbicide tolerance and unbolting).

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Fig. 2

Positive rates in the patch testing. Nickel, Nickel sulfate as a positive control; NC, blanked as a negative control; WT, wild type of Zoysia japonica; 1, JG21 (genetically modified Z. japonica for herbicide tolerance); 2, JG21-MS2 (genetically modified Z. japonica for herbicide tolerance and male sterility); 3, JG21-MS1 (genetically modified Z. japonica for herbicide tolerance and unbolting).

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Table 1

Incidences of skin reaction to test objects among 97 participants

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Test objects ± 1+ 2+ 3+
Nickel sulfate 1 19 14 5
Blanked 0 0 0 0
Leaves of Z. japonica
 Wild type 5 8 3 0
 JG21 5 8 2 0
 JG21-MS2 5 8 2 0
 JG21-MS1 4 8 2 0

Z. japonica, Zoysia japonica.

Notes

This work was supported by grants from the Next-Generation BioGreen 21 Program (PJ009499032014), Rural Development Administration, Republic of Korea and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2009-0094059).

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