A comparative analysis of micro-organism colonization of tooth-brushes in a public bath based on the storage technique

Jung Won Yun; Youn Kyung Kim; Ji Su Kim; Min Kyung Lee; Jung Hwa Lee

doi:10.11149/jkaoh.2017.41.4.250

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Yun, Kim, Kim, Lee, and Lee: A comparative analysis of micro-organism colonization of tooth-brushes in a public bath based on the storage technique

Original Article

Journal of Korean Academy of Oral Health 2017; 41(4): 250-254.

Published online: 31 December 2017

DOI: https://doi.org/10.11149/jkaoh.2017.41.4.250

A comparative analysis of micro-organism colonization of tooth-brushes in a public bath based on the storage technique

Jung Won Yun¹, Youn Kyung Kim¹, Ji Su Kim², Min Kyung Lee³, Jung Hwa Lee³

¹Department of Biomedical Health Science, Graduate School, Dong-Eui University, Busan, Korea.

²Department of Preventive & Community Dentistry, Pusan National University, Yangsan, Korea.

³Department of Dental Hygiene, College of Nursing, Healthcare Sciences & Human Ecology, Dong-Eui University, Busan, Korea.

Corresponding Author: Jung Hwa Lee. Department of Dental Hygiene, College of Nursing, Healthcare Sciences & Human Ecology, Dong-Eui University, 176 Eomgwang-ro, Busanjin-gu, Busan 47340, Korea. Tel: +82-51-890-4239, Fax: +82-505-182-687, yamako93@deu.ac.kr

Received 16 August 2017 Revised 12 December 2017 Accepted 13 December 2017

Journal of Korean Academy of Oral Health

(open-access):

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives

Bacterial colonization on toothbrushes can cause stomatitis and other oral diseases such as dental caries and periodontitis. In this study we tested 18 toothbrushes, of which six were left exposedto the air, six were stored in a plastic bag, and six in a toothbrush case.

Methods

ATP (RLU: relatively light unit) values measured by ATP Luminometer were compared with APC (CFU: colony forming unit) by swabbing culture method using 3M™ petrifilm™ aerobic count plates.

Results

Swabs from toothbrushes left exposed in the air developed an average of 651.67±261.94 CFU, while the “In plastic bag” and the “In toothbrush case” specimens developed 593.17±354.33 and 120.83±68.87 CFU, respectively.

Conclusion

The best option is to rinse the toothbrush well with water and to keep them dry in a well-ventilated area in order to reduce the growth of bacteria in a public bath.

Keywords: ATP bioluminescence, Micro-organism, Public bath, Toothbrush

Figures and Tables

Fig. 1

ATP measurement according to storage method of the toothbrush.

Fig. 2

ATP measurement according to storage method of the tooth-brush.

Table 1

ATP measurement according to storage method of the toothbrush

RLU: Relatively Light Unit.

^*P<0.05.

Table 2

APC measurement according to storage method of the toothbrush

CFU: Colony Forming Unit.

Table 3

APC & ATP measurement according to storage method & time of the tooth-brush

RLU: Relatively Light Unit. CFU: Colony Forming Unit.

^***P<0.001.

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