Bacteroides nordii and Bacteroides salyersiae Isolated from Post-operative Peritonitis Patients

Jihoon G. Yoon; Hyunsoo Kim; Yangsoon Lee; Myungsook Kim; Dongeun Yong; Seok Hoon Jeong; Kyungwon Lee

doi:10.3343/lmo.2016.6.2.111

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Yoon, Kim, Lee, Kim, Yong, Jeong, and Lee: Bacteroides nordii and Bacteroides salyersiae Isolated from Post-operative Peritonitis Patients

Case Report

Clinical Microbiology

Laboratory Medicine Online 2016; 6(2): 111-115.

Published online: 1 April 2016

DOI: https://doi.org/10.3343/lmo.2016.6.2.111

Bacteroides nordii and Bacteroides salyersiae Isolated from Post-operative Peritonitis Patients

Jihoon G. Yoon, M.D.¹, Hyunsoo Kim, M.D.¹, Yangsoon Lee, M.D.², Myungsook Kim, M.T.¹, Dongeun Yong, M.D.¹, Seok Hoon Jeong, M.D.¹, Kyungwon Lee, M.D.¹

¹Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea.

²Department of Laboratory Medicine, Hanyang University College of Medicine, Seoul, Korea.

Corresponding author: Kyungwon Lee. Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Korea. Tel: +82-2-2228-2446, Fax: +82-2-313-0908, leekcp@yuhs.ac

Received 24 June 2015 Revised 13 July 2015 Accepted 14 July 2015

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In this study, we report three cases in which two species of the Bacteroides fragilis group, 'Bacteroides nordii' and 'Bacteroides salyersiae', were isolated from peritoneal fluid cultures from post-operative peritonitis patients. The two species of the B. fragilis group were initially misidentified as B. fragilis/Bacteroides stercoris and Bacteroides ovatus by Rapid ID 32A (bioMé rieux, France), and finally confirmed as B. nordii and B. salyersiae using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16s rRNA sequencing. For the identification of anaerobes, particularly B. fragilis group organisms, MALDI-TOF MS is a useful method not only because of its concordance with 16S rRNA sequencing results, but also because of its rapidity and simple procedure.

Keywords: Bacteroides fragilis group, Bacteroides nordii, Bacteroides salyersiae, Spectrometry, Mass, Matrix-assisted laser desorption-ionization

Figures and Tables

Table 1

Results of peritoneal fluid culture and species identification using three methods

Case No. (Lab. No.)	Sex/age	Underlying disease	Aerobes	Anaerobes identified by
Case No. (Lab. No.)	Sex/age	Underlying disease	Aerobes	Rapid ID 32A	MALDI-TOF MS	16S rRNA gene sequencing
1 (2011-10-C174)	M/78	Rectal cancer	Few E. faecalis	Few B. fragilis/B. stercoris	B. nordii 1.987^*	B. nordii 98.80%^†
2 (2011-01-C238)	F/74	Sigmoid colon cancer	Some P. aeruginosa Few E. faecium	Many B. ovatus	B. salyersiae 2.098^*	B. salyersiae 99.62%^†
3 (2012-04-C835)	M/78	Rectal cancer	Few E. coli	Few B. ovatus Few E. lenta	B. salyersiae 2.106^* NT	B. salyersiae 99.71%^† NT

^*Score value determined using Bruker MALDI Biotyper software package (version 3.1) with the reference database version 4.0.0.1; ^†Pairwise similarity (%) determined using EzTaxon-e database (http://www.eztaxon.org/).

Abbreviation: NT, not tested.

Table 2

Results of antimicrobial susceptibilities of B. nordii and B. salyersiae

Case No.	1		2		3
Species	B. nordii		B. salyersiae		B. salyersiae
Species	MIC (µg/mL)	Susceptibility	MIC (µg/mL)	Susceptibility	MIC (µg/mL)	Susceptibility
Piperacillin	512	R	256	R	4	S
PIP-TZ	1	S	0.03	S	0.03	S
Cefoxitin	32	I	32	I	16	S
Cefotetan	64	R	128	R	8	S
Imipenem	0.25	S	0.5	S	0.125	S
Meropenem	0.5	S	0.5	S	0.25	S
Clindamycin	0.03	S	256	R	2	S
Moxifloxacin	8	R	0.5	S	1	S
Chloramphenicol	4	S	8	S	4	S
Metronidazole	2	S	1	S	1	S
Tigecycline	0.25	NA	1	NA	16	NA

Abbreviations: PIP-TZ, piperacillin-tazobactam; S, susceptible; I, intermediate; R, resistant; NA, not applicable.

Notes

This article is available from http://www.labmedonline.org

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