PCR-based Investigation of Infection Patterns in Patients with Pelvic Inflammatory Diseases in Jeju

Woo Jin Kim; Kyutaeg Lee; Dong Lyul Kim

doi:10.3343/lmo.2013.3.2.75

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Kim, Lee, and Kim: PCR-based Investigation of Infection Patterns in Patients with Pelvic Inflammatory Diseases in Jeju

Original Article

Clinical Microbiology

Laboratory Medicine Online

Laboratory Medicine Online 2013; 3(2): 75-78.

Published online: 1 April 2013

DOI: https://doi.org/10.3343/lmo.2013.3.2.75

PCR-based Investigation of Infection Patterns in Patients with Pelvic Inflammatory Diseases in Jeju

Woo Jin Kim, M.D., Kyutaeg Lee, M.D., Dong Lyul Kim, M.D

Department of Laboratory Medicine, Cheju Halla General Hospital, Jeju, Korea.

Corresponding author: Woo Jin Kim, M.D. Department of Laboratory Medicine, Cheju Halla General Hospital, Doryeong-ro 65, Jeju-si Jeju Special Self-Governing Province 690-766, Korea. Tel: +82-64-740-5256, Fax: +82-64-740-5332, oojinkim@hanmail.net

Received 13 June 2012 Revised 30 July 2012 Accepted 2 August 2012

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Pelvic inflammatory disease (PID) is a microbial infection caused by the upward spread of infectious organisms through the cervical os. Early diagnosis and treatment of PID are essential for the prevention of sequelae such as ectopic pregnancies, infertility, and chronic pelvic pain. Although Chlamydia trachomatis and Neisseria gonorrhoeae are well-known causal agents of PID, there have been reports on some changes in PID-associated infection. The aim of this study was to investigate the infection patterns in patients with PID in Jeju.

Methods

Endocervical samples obtained from 65 patients with PID were tested for C. trachomatis, Mycoplasma genitalium, Mycoplasma hominis, N. gonorrhoeae, Trichomonas vaginalis, and Ureaplasma urealyticum using multiplex PCR.

Results

The samples were positive for C. trachomatis (63%), M. hominis (34%), U. urealyticum (20%), M. genitalium (17%), N. gonorrhoeae (9%), and T. vaginalis (6%).

Conclusions

This study showed that C. trachomatis infection was prevalent and the incidence of M. hominis was higher than that of U. urealyticum.

Keywords: Pelvic inflammatory disease, Chlamydia trachomatis, PCR

Figures and Tables

Table 1

Incidence of monomicrobial, dimicrobial, and polymicrobial infection in 35 patients with pelvic inflammatory disease

Abbreviations: CT, Chlamydia trachomatis; MH, Mycoplasma hominis; NG, Neisseria gonorrhoeae; UU, Ureaplasma urealyticum; MG, Mycoplasma genitalium; TV, Trichomonas vaginalis.

Table 2

Total number of microorganisms in the 35 patients with pelvic inflammatory disease including overlapping results

Notes

This article is available from http://www.labmedonline.org

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