Basic Data for Reference Intervals in Koreans for Parameters Produced by Multiplate Platelet Function Analyzer

Sae Yun Baik; Ji Man Hong; Young Ae Lim

doi:10.3343/lmo.2013.3.4.191

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Baik, Hong, and Lim: Basic Data for Reference Intervals in Koreans for Parameters Produced by Multiplate Platelet Function Analyzer

Original Article

Diagnostic Hematology

Laboratory Medicine Online 2013; 3(4): 191-197.

Published online: 1 October 2013

DOI: https://doi.org/10.3343/lmo.2013.3.4.191

Basic Data for Reference Intervals in Koreans for Parameters Produced by Multiplate Platelet Function Analyzer

Sae Yun Baik, M.D.¹, Ji Man Hong, M.D.², Young Ae Lim, M.D.³

¹Green Cross Laboratories, Yongin, Korea.

²Department of Neurology, Ajou University School of Medicine, Suwon, Korea.

³Department of Laboratory and Medicine, Ajou University School of Medicine, Suwon, Korea.

Corresponding author: Young Ae Lim. Department of Laboratory Medicine, Ajou University School of Medicine, 164 World cup-ro, Yeongtong-gu, Suwon 443-721, Korea. Tel: +82-31-219-5786, Fax: +82-31-219-5778, limyoung@ajou.ac.kr

Received 10 September 2012 Revised 11 December 2012 Accepted 14 December 2012

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

The Multiplate analyzer (Dynabyte GmbH) has been recently introduced as a platelet function test for patients taking antiplatelet drugs. The study aimed at providing basic data for determining the reference interval of parameters produced by Multiplate in Koreans and to study the factors that influence those parameters.

Methods

Blood was collected from 35 healthy volunteers (female 18, male 17) into tubes containing hirudin or 3.2% sodium citrate. Whole blood platelet aggregations triggered by adenosine-5'-diphosphate (ADP), ADP-high sensitive (ADP+PGE1 only in hirudin samples), arachidonic acid (AA), collagen or thrombin receptor activator peptide (TRAP) were investigated using Multiplate according to the manufacturer's instructions. Data from healthy volunteers for the area under the curve (AUC) were determined from the central 95th percentile of the results.

Results

The values of AUC in hirudin samples for all agonists were significantly higher than those in sodium citrate samples. The AUC values in hirudin (sodium citrate) samples were as follows: ADP 38-107 (18-119) U; ADP+PGE1 16-91 U; AA 64-156 (32-117) U; collagen 53-112 (26-108) U; and TRAP 81-163 (49-149) U. The parameters from Multiplate were significantly correlated with leukocyte counts, but not with hematocrit levels.

Conclusions

Although our data were derived from only 35 subjects, the results are expected to be helpful in determining the reference interval at a single institute and may serve as basic data for future cumulative data of reference intervals from multiple institutes in Korea.

Keywords: Reference intervals, platelet function, Multiplate analyzer

Figures and Tables

Table 1

Comparison and Pearson's correlations (R value) for parameters from the Multiplate analyzer according to the anticoagulants and platelet-aggregating agents

^*P values for data of hirudin versus Na-citrate by Student's t-test; ^†P<0.001; ^‡P<0.05.

Abbreviations: ADP, adenosine diphosphate; AA, arachidonic acid; TRAP, thrombin receptor activator peptide; AUC, area under the curve.

Table 2

Reference range (central 95th percentile) for parameters from the Multiplate analyzer according to the anticoagulants and aggregating agents

Abbreviations: ADP, adenosine diphosphate; PGE1, prostaglandin E1; AA, arachidonic acid; TRAP, thrombin receptor activator peptide; AUC, area under the curve; ND, not done.

Table 3

Pearson's correlations between parameters from the Multiplate analyzer according to the anticoagulants and platelet-aggregating agents

All correlation coefficiencts were significant (P<0.001).

For abbreviations and final concentration of aggregating agents, see Table 2.

Table 4

Pearson's correlations between hematologic parameters, age and AUC (U) according to the anticoagulants and aggregating agents

^*P<0.001; ^†P<0.01; ^‡P<0.05.

For abbreviation and final concentration of aggregating agents, see Table 2.

Table 5

Comparison of reference intervals of AUC (U) according to data from references

For abbreviations, see Table 2.

^*The number of individuals for the reference intervals of AUC differs according to agonist.

Notes

This article is available from http://www.labmedonline.org

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