Won Young Jin; Sook Jin Jang; Young Sook Kim; Geon Park; Dae Soo Moon; Young Jin Park

doi:10.3343/lmo.2012.2.1.10

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Jin, Jang, Kim, Park, Moon, and Park: Comparison of MicroScan and Phoenix Automated Systems for Detection of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae

Short Communication

Clinical Microbiology

Laboratory Medicine Online

Laboratory Medicine Online 2012; 2(1): 55-58.

Published online: 1 January 2012

DOI: https://doi.org/10.3343/lmo.2012.2.1.10

Comparison of MicroScan and Phoenix Automated Systems for Detection of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae

Won Young Jin, M.D.¹, Sook Jin Jang, M.D.^1,², Young Sook Kim, M.D.³, Geon Park, M.D.¹, Dae Soo Moon, M.D.¹, Young Jin Park, M.D.¹

¹Department of Laboratory Medicine, Chosun University College of Medicine, Gwangju, Korea.

²Research Center for Resistant Cells, Chosun University, Gwangju, Korea.

³Department of Diagnostic Radiology, Chosun University College of Medicine, Gwangju, Korea.

Corresponding author: Sook Jin Jang, M.D. Department of Laboratory Medicine, Chosun University Hospital, 588 Seoseok-dong, Dong-gu, Gwangju 501-717, Korea. Tel: +82-10-9614-8853, Fax: +82-62-232-2063, sjbjang@chosun.ac.kr

Received 13 October 2011 Revised 16 October 2011 Accepted 17 October 2011

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to evaluate the performances of MicroScan (Siemens Healthcare, USA) and Phoenix (Becton Dickinson Diagnostic Systems, USA) automated systems for the detection of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae. ESBL-producers were detected from 18 E. coli strains and 26 K. pneumoniae strains using MicroScan, Phoenix, and double-disk synergy test (DDST). The ESBL types were determined by PCR direct sequencing. ESBL genes were detected in 38 (86.4%) of the 44 test strains. The sensitivities of MicroScan, Phoenix, and DDST were 94.6%, 79%, and 89.5%, respectively. Both MicroScan and Phoenix provided acceptable results for the examination of clinical isolates.

Keywords: Extended-spectrum β-lactamase, Escherichia coli, Klebsiella pneumoniae, MicroScan, Phoenix, Double-disk synergy test

Figures and Tables

Table 1

Characterization of ESBL-producing clinical isolates (N=44) and results of ESBL detection performed using MicroScan, Phoenix, and DDST

^*Possible ESBL: confirmation test required to distinguish AmpC from ESBL.

Abbreviations: ESBL, extended-spectrum β-lactamase; ECO, E. coli; KPN, K. pneumoniae; DDST, double-disk synergy test; NT, not tested.

Notes

This article is available from http://www.labmedonline.org

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