Analysis of Polycheck Allergy Results of the Recent Two Years: Comparison with Skin Prick Test and ImmunoCAP

Seri Jeong; Gwang-Cheon Jang; Nam Joon Cho; Mi Soon Han; Hyon Suk Kim; Je Young Sun; Jong-Ha Yoo

doi:10.3343/lmo.2012.2.3.139

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Jeong, Jang, Cho, Han, Kim, Sun, and Yoo: Analysis of Polycheck Allergy Results of the Recent Two Years: Comparison with Skin Prick Test and ImmunoCAP

Original Article

Diagnostic Immunology

Laboratory Medicine Online

Laboratory Medicine Online 2012; 2(3): 139-147.

Published online: 1 July 2012

DOI: https://doi.org/10.3343/lmo.2012.2.3.139

Analysis of Polycheck Allergy Results of the Recent Two Years: Comparison with Skin Prick Test and ImmunoCAP

Seri Jeong, M.D.^1,^4,^*, Gwang-Cheon Jang, M.D.^2,^*, Nam Joon Cho, M.D.³, Mi Soon Han, M.D.^1,⁴, Hyon Suk Kim, M.D.⁴, Je Young Sun, ⁵, Jong-Ha Yoo, M.D.^1,⁴

¹Department of Laboratory Medicine, National Health Insurance Corporation Ilsan Hospital, Goyang, Korea.

²Department of Pediatrics, National Health Insurance Corporation Ilsan Hospital, Goyang, Korea.

³Department of Dermatology, National Health Insurance Corporation Ilsan Hospital, Goyang, Korea.

⁴Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.

⁵Department of Biochemistry and Cell Biology, Rice University, Houston, Texas, USA.

Corresponding author: Jong-Ha Yoo, M.D. Department of Laboratory Medicine, National Health Insurance Corporation Ilsan Hospital, 1232 Baekseok-dong, Ilsandong-gu, Goyang 410-719, Korea. Tel: +82-31-900-0909, Fax: +82-31-900-0925, jhyoo92@empal.com

Equal contributor:

*These authors equally contributed to this work.

Received 19 December 2011 Revised 27 March 2012 Accepted 3 April 2012

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Multiple Antigen Simultaneous Test (MAST)-immunoblot assay is a practical and economical test, which has been recently introduced nationwide. Authors investigated test efficiency of a MAST-immunoblot assay, Polycheck Allergy (PA).

Methods

A total of 3,153 patients were tested by PA and the results were compared with the results of ImmunoCAP and skin prick test (SPT) in 532 and 75 patients, respectively. The correlation with the lgE results measured by VIDAS was also analyzed.

Results

The agreements of PA with SPT were 87.8% in the Inhalant Panel and 89.3% in the Food Panel and the agreement of ImmunoCAP with SPT was 95.2%. The most common allergens giving positive reactions were Dermatophagoides farinae (46.2%) and Dermatophagoides pteronyssinus (40.0%). SPT taken as a reference, PA compared with ImmunoCAP showed higher agreement (D. farinae, 76.0 vs. 70.7%; D. pteronyssinus, 76.0 vs. 74.4%), sensitivity (D. farinae, 72.7 vs. 68.2%; D. pteronyssinus, 75.0 vs. 71.2%) and specificity (D. farinae, 85.0 vs. 81.3%) except for the specificity for D. pteronyssinus (78.3 vs. 87.5%). The rate of allergen specific IgE positive patients was higher than that of negative patients when total IgE was over 100 kU/L.

Conclusions

Our results showed that the agreement, sensitivity and specificity of PA were similar to or better than those of the previously established test, ImmunoCAP. The allergen specific IgE results of PA were in correlation with total IgE. PA may be used for testing allergen specific IgE to diagnose and treat allergic diseases.

Keywords: Allergy, Polycheck Allergy, ImmunoCAP, Skin prick test, Allergen-specific IgE

Figures and Tables

Fig. 1

Polycheck Allergy results plotted against ImmunoCAP results. Correlation coefficients (r²) between ImmunoCAP and Polycheck Allergy for Dermatophagoides farina and Dermatophagoides pteronyssinus were 0.46 and 0.43, respectively (each, P<0.001).

Fig. 2

Number of allergen-specific IgE detected simultaneously by Polycheck Allergy. The number of most frequently detected allergen-specific IgE was 3 (23.3%, 124/532) in inhalant panel and 1 (14.1%, 75/532) in food panel.

Table 1

Age and sex distribution of patients tested with Polycheck Allergy, ImmunoCAP, and skin prick test

Table 2

Positive rate of each allergen-specific IgE detected by Polycheck Allergy, skin prick test, and ImmunoCAP

^*Allergen in Polycheck Allergy Inhalant Panel; ^†in Skin prick test; ^‡in Food Panel.

Table 3

Agreement, sensitivity, and specificity of each multiple allergen simultaneous test compared with skin prick test as a reference

^*Allergen in Polycheck Allergy Inhalant Panel; ^†in Skin prick test; ^‡in Food Panel; ^§Mean percentage of Polycheck Allergy Inhalant Panel/Food Panel.

Table 4

Comparison of Polycheck Allergy with ImmunoCAP as a reference

Table 5

Comparison of Polycheck Allergy with skin prick test as a reference

Table 6

Distribution of serum total IgE levels determined by Polycheck Allergy in all allergen-specific IgE positive and negative patients

Notes

This article is available from http://www.labmedonline.org

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