Evaluation of an Automated Coagulation Analyzer Coapresta 2000

Jonghyeon Choi; Sungwook Song; Yongjung Park; Jong Rak Choi; Jaewoo Song

doi:10.3343/lmo.2011.1.2.5

Journal List > Lab Med Online > v.1(2) > 1057150

Go to TopGo to Top Go to BottomGo to Bottom

TOOLS

Choi, Song, Park, Choi, and Song: Evaluation of an Automated Coagulation Analyzer Coapresta 2000

Original Article

Diagnostic Hematology

Laboratory Medicine Online

Laboratory Medicine Online 2011; 1(2): 94-99.

Published online: 1 April 2011

DOI: https://doi.org/10.3343/lmo.2011.1.2.5

Evaluation of an Automated Coagulation Analyzer Coapresta 2000

Jonghyeon Choi, Sungwook Song, Yongjung Park, Jong Rak Choi, Jaewoo Song

Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.

Corresponding author: Jaewoo Song. Department of Laboratory Medicine, Yonsei University College of Medicine, 250 Seongsan-ro, Seodaemun-gu, Seoul 120-752, Korea. Tel: +82-2-2228-2445, Fax: +82-2-313-0956, labdx@yuhs.ac

Received 10 August 2010 Revised 10 February 2011 Accepted 11 February 2011

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

The Coapresta 2000 (Sekisui Medical CO., LTD, Japan) is a fully automated random-access multiparameter hemostasis coagulation analyzer, which is equipped with a photo-optical clot detection unit and a cap-piercing system. It is able to perform clotting time assays as well as colorimetric assays (synthetic substrate method and latex turbidimetric method). In this study, we evaluated the analytical performance of the Coapresta 2000 for coagulation test items and compared with that of the ACL-TOP (Instrumentation Laboratory, Lexingtion, MA, USA) analyzer, which is currently used for routine coagulation test items in our hospital.

Methods

The Coapresta 2000 was evaluated with respect to its technical characteristics in the determination of 8 routine coagulation test items: prothrombin time, activated partial thromboplastin time, fibrinogen, fibrin-degradation product (FDP) antithrombin III, D-dimer, factors VIII and IX. Analyse-it (Analyse-it Software Ltd, UK) and SigmaStat (Systat Software, Inc., USA) were used for statistical analysis between items on the Coapresta 2000 and the ACL-TOP analyzer.

Results

The intra-assay and inter-assay coefficients of variation (CV) were below 5% for both groups of samples having values within the reference interval and outside the reference interval. Significant interference was observed with hemolytic and icteric samples. Carryover was not detected. The results obtained by Coapresta 2000 were well correlated with those obtained by the ACL-TOP analyzer (r² in the range from 0.781 to 0.969).

Conclusions

We concluded that Coapresta 2000 analyzer was well correlated with ACL-TOP analyzer for the routine coagulation test items tested.

Keywords: Coapresta 2000, Evaluation, Correlation, ACL-TOP

Figures and Tables

Fig. 1

Correlation of the test results between ACL-TOP and CP 2000 analyzers for 8 coagulation test items.

Fig. 2

Bias of the test results between ACL-TOP and CP 2000 analyzers for 8 coagulation test items.

Table 1

Reagents and calibrators used in this study for comparison of two coagulation analyzers

Table 2

Intra-assay & inter-assay precisions of the CP 2000 analyzer

Table 3

Comparison of the results of coagulation tests performed on the ACL-TOP and the CP 2000 analyzers

Results are expressed as the median values with ranges in parentheses.

Notes

This article is available from http://www.labmedonline.org

References

1. Walenga JM, Fareed J. Automation and quality control in the coagulation laboratory. Clin Lab Med. 1994. 14:709–728.

2. Amiral J, Adalbert B, Adam M. Application of enzyme immunoassays to coagulation testing. Clin Chem. 1984. 30:1512–1516.

3. Fischer F, Appert-Flory A, Jambou D, Toulon P. Evaluation of the automated coagulation analyzer Sysmex CA-7000. Thromb Res. 2006. 117:721–729.

4. Appert-Flory A, Fischer F, Jambou D, Toulon P. Evaluation and performance characteristics of the automated coagulation analyzer ACL TOP. Thromb Res. 2007. 120:733–743.

5. Kim YA, Sun YK. Measurement of PT, aPTT, and fibrinogen by automatic coagulation analyzer, ACL9000. J Lab Med Qual Assur. 2002. 24:201–207.

6. Kim HS, Nam KH, Lee DH. Measurement of PT, APTT, and fibrinogen by automatic coagulation analyzer, Sysmex CA-540. J Clin Pathol Qual Control. 2001. 23:247–252.

7. Lee YW, Chang CW, Lim MS, Lim BJ, Lee YK. Laboratory evaluation of automated coagulation analyzers sysmex CA-1500TM and CA-7000TM. J Clin Pathol Qual Control. 2001. 23:253–258.

8. Clinical and Laboratory Standards Institute. CLSI Document H47-A2. One-stage prothrombin time (PT) test and activated partial thromboplastin time (APTT) test; approved guideline. 2008. Wayne, PA: Clinical and Laboratory Standards Institute.

9. National Committee for Clinical Laboratory Standards. NCCLS Document H30-A2. Procedure for the determination of fibrinogen in plasma; approved guideline-second edition. 2001. Wayne, PA: National Committee for Clinical Laboratory Standards.

10. Milos M, Herak D, Kuric L, Horvat I, Zadro R. Evaluation and performance characteristics of the coagulation system: ACL TOP analyzer-HemosIL reagents. Int J Lab Hematol. 2009. 31:26–35.

11. Eschwège V, Catillon C, Robert A. Evaluation of the automated coagulation analyser ACL TOP (instrumentation laboratory). Ann Biol Clin (Paris). 2006. 64:259–264.

TOOLS

Similar articles