Hye Jin Lee; Ha Nui Kim; Byong Joon Yoo; Jang Su Kim; Myong Han Kim; Chae Seung Lim; Kap No Lee

doi:10.3343/lmo.2011.1.2.6

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Lee, Kim, Yoo, Kim, Kim, Lim, and Lee: Evaluation of the LG Advansure™ Malaria P.f./P.v. real-time QPCR for the Diagnosis of Malaria

Original Article

Clinical Microbiology

Laboratory Medicine Online

Laboratory Medicine Online 2011; 1(2): 100-104.

Published online: 1 April 2011

DOI: https://doi.org/10.3343/lmo.2011.1.2.6

Evaluation of the LG Advansure™ Malaria P.f./P.v. real-time QPCR for the Diagnosis of Malaria

Hye Jin Lee, Ha Nui Kim, Byong Joon Yoo, Jang Su Kim, Myong Han Kim, Chae Seung Lim, Kap No Lee

Department of Laboratory Medicine, Korea University College of Medicine, Seoul, Korea.

Corresponding author: Chae Seung Lim. Dapartment of Laboratory Medicine, Korea University Guro Hospital, Guro 2-dong, Guro-gu, Seoul 152-703, Korea. Tel: +82-2-2626-1455, Fax: +82-2-2626-1465, malarim@korea.ac.kr

Received 21 October 2010 Revised 31 January 2011 Accepted 31 January 2011

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Malaria is a problematic disease in Korea, and microscopic examination of Giemsa-stained blood smear has been used as the gold standard for its diagnosis. However, this technique is time-consuming and has low sensitivity in samples with low numbers of malarial parasites (<20 parasites/µL). Here, we evaluated the performance characteristics of the LG Advansure™ Malaria P.f./P.v. real-time QPCR (LG life sciences, Korea).

Methods

Blood samples from 173 persons who visited Korea University Ansan Hospital were evaluated. QPCR was performed in 73 malaria patients and 100 healthy subjects by using the LG Advansure Malaria P.f./P.v. real-time QPCR^R kit, and the results were compared with those of microscopy. The detection limit of this kit was determined by serial dilution of Plasmodium-infected blood with normal blood (blood not infected with Plasmodium).

Results

Among the 73 patients that were microscopically confirmed to have malaria (Plasmodium vivax infection, N=70, P. falciparum infection, N=3), 69 patients were diagnosed with P. vivax infection and 3 were diagnosed with P. falciparum infection by LG Advansure™ Malaria P.f./P.v. real-time QPCR. Both the tests indicated absence of infection in the 100 healthy subjects. The detection limit of LG Advansure™ Malaria P.f./P.v. real-time QPCR was 0.1 parasite/µL.

Conclusions

LG Advansure™ Malaria P.f./P.v. real-time QPCR is a very sensitive and specific technique and can be used as a confirmatory test for malaria.

Keywords: Malaria, Real time quantitative PCR, Diagnosis

Figures and Tables

Table 1

Comparison of the results of real time quantitative PCR and microscopic examination of Giemsa-stained blood film

^*Plasmodium vivax; ^†Plasmodium falciparum.

Table 2

Detection limit of Plasmodium falciparum parasitemia by real time quantitative PCR

Abbreviations: P, positive; N, negative.

Notes

This article is available from http://www.labmedonline.org

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