Alpha-Lipoic Acid Decreases Iodine Uptake in the Rat Thyroid Cell Line FRTL-5

Ji Hye Yim; Tae Yong Kim; Sergio Ruiz-Llorente; Won Gu Kim; Ji Min Han; Min Ji Jeon; Won Bae Kim; Young Kee Shong

doi:10.0000/jkta.2012.5.1.39

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Yim, Kim, Ruiz-Llorente, Kim, Han, Jeon, Kim, and Shong: Alpha-Lipoic Acid Decreases Iodine Uptake in the Rat Thyroid Cell Line FRTL-5

Original Article

Journal of Korean Thyroid Association

Journal of Korean Thyroid Association 2012; 5(1): 39-44.

Published online: 31 May 2012

DOI: https://doi.org/10.0000/jkta.2012.5.1.39

Alpha-Lipoic Acid Decreases Iodine Uptake in the Rat Thyroid Cell Line FRTL-5

Ji Hye Yim, MD¹, Tae Yong Kim, MD, PhD¹, Sergio Ruiz-Llorente, PhD², Won Gu Kim, MD, PhD¹, Ji Min Han, MD¹, Min Ji Jeon, MD¹, Won Bae Kim, MD, PhD¹, Young Kee Shong, MD, PhD¹

¹Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

²Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

Corresponding author (tykim@amc.seoul.kr)

Received 8 September 2011 Revised 27 February 2012 Accepted 7 March 2012

Abstract

Background and Objectives

Alpha-lipoic acid (ALA) is a naturally occurring fatty acid with strong antioxidant properties that exerts protective effects against harmful free radical damage. The aim of the present study was to assess the effect of ALA on iodine uptake and expression of sodium iodine symporter (NIS) using FRTL-5 cells.

Materials and Methods

Cells were treated with ALA for various time and doses, in the presence or absence of thyrotropin (TSH). Cell viability assay, iodine uptake assay and NIS promoter activity assay were performed.

Results

ALA increased NIS promoter activity. It showed an additive effect when concomitantly added with TSH. After 48 hours of incubation with ALA in the presence or absence of TSH, there was no difference in iodine uptake according to doses of ALA. After 72 hours of incubation with ALA and TSH, ALA decreased iodine uptake in dose-dependent way.

Conclusion

ALA induced NIS gene transcription of FRTL-5, but suppressed iodine uptake in the presence of TSH. ALA may suppress iodine uptake through effect for post-translation stage of NIS protein.

Keywords: Thyroid, Alpha-lipoic acid, Sodium iodine symporter

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