Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth

Su-Jung Shin; Woocheol Lee; Jae-Il Lee; Seung-Ho Baek; Kee-Yeon Kum; Won-Jun Shon; Kwang-Shik Bae

doi:10.5395/JKACD.2011.36.3.196

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Shin, Lee, Lee, Baek, Kum, Shon, and Bae: Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth

Basic Research

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2011; 36(3): 196-202.

Published online: 31 May 2011

DOI: https://doi.org/10.5395/JKACD.2011.36.3.196

Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth

Su-Jung Shin, DDS, MS¹, Woocheol Lee, DDS, PhD², Jae-Il Lee, DDS, MDS, PhD³, Seung-Ho Baek, DDS, PhD², Kee-Yeon Kum, DDS, PhD², Won-Jun Shon, DDS, PhD², Kwang-Shik Bae, DDS, MDS, PhD²

Assistant Professor

Associate Professor

Professor

Associate Professor

Assistant Professor

Professor

¹Department of Conservative Dentistry, College of Dentistry, Yonsei University, Seoul, Korea.

²Department of Conservative Dentistry, Dental Research Institute, Seoul, Korea.

³Department of Oral Pathology, School of Dentistry, Seoul National University, Seoul, Korea.

Correspondence to Kwang-Shik Bae, DDS, MDS, PhD. Professor, Department of Conservative Dentistry, Seoul National University School of Dentistry and Dental Research Institute, 28-2 Yeongeon-dong, Jongro-gu, Seoul, Korea 110-749. TEL, +82-2-2072-2651; FAX, +82-2-764-3859; baeks@snu.ac.kr

Received 10 March 2011 Revised 4 April 2011 Accepted 4 April 2011

Abstract

Objectives

The aim of this study was to investigate levels of matrix metalloproteinase-8 (MMP-8) and substance P (SP) in root canal exudates during root canal treatment (RCT) of nonvital, painful teeth.

Materials and Methods

Patients scheduled for nonsurgical RCT were prospectively selected; the study was performed after obtaining informed consent from the patients and was approved by the Institutional Review Board for Clinical Research of Gangnam Severance Hospital, Yonsei University (3-2008-0118). Canal exudates samples were collected using sterilized paper points from teeth scheduled for RCT across three different time periods. MMP-8 and SP levels were measured using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a mixed model analysis and the Pearson correlation analysis (p < 0.05).

Results

MMP-8 and SP levels in GCF were decreased during RCT (p < 0.0001), and they showed a weak positive correlation to each other (p < 0.05). Patients'subjective pain levels and the response from percussion test were significantly related to SP level.

Conclusions

This study demonstrated that periradicular inflammation endodontic origin can elevate SP and MMP-8 levels in root canal exudates. Interestingly, SP level of canal exudates showed a possibility of being used as an indicator of pain due to periapical pathosis.

Keywords: Canal exudate, MMP-8, SP, Pain

Figures and Tables

Figure 1

The amount of MMP-8 and SP from individual samples.

Figure 2

Corrected estimates and standard errors of MMP-8 and SP levels in the experimental group. A mixed model was used for adjusting patients' pain level.

Figure 3

Scatter plot of MMP-8 and SP at each period. (a) At first visit, (b) At second visit, (c) At third visit.

References

1. Heyeraas KJ, Kvinnsland I, Byers MR, Jacobsen EB. Nerve fibers immunoreactive to protein gene product 9.5, calcitonin gene-related peptide, substance P, and neuropeptide Y in the dental pulp, periodontal ligament, and gingiva in cats. Acta Odontol Scand. 1993. 51:207–221.

2. Ağan S, Sönmez S, Serdar M. The effect of topical doxycycline usage on gingival crevicular fluid MMP-8 levels of chronic and aggressive periodontitis patients: a pilot study. Int J Dent Hyg. 2006. 4:114–121.

3. Biyikoğlu B, Buduneli N, Kardeşler L, Aksu K, Pitkala M, Sorsa T. Gingival crevicular fluid MMP-8 and -13 and TIMP-1 levels in patients with rheumatoid arthritis and inflammatory periodontal disease. J Periodontol. 2009. 80:1307–1314.

4. Hanioka T, Takaya K, Matsumori Y, Matsuse R, Shizukuishi S. Relationship of the substance P to indicators of host response in human gingival crevicular fluid. J Clin Periodontol. 2000. 27:262–266.

5. Hanemaaijer R, Sorsa T, Konttinen YT, Ding Y, Sutinen M, Visser H, van Hinsbergh VW, Helaakoski T, Kainulainen T, Rönkä H, Tschesche H, Salo T. Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells. Regulation by tumor necrosis factor-alpha and doxycycline. J Biol Chem. 1997. 272:31504–31509.

6. Palosaari H, Wahlgren J, Larmas M, Rönkä H, Sorsa T, Salo T, Tjäderhane L. The expression of MMP-8 in human odontoblasts and dental pulp cells is down-regulated by TGF-beta1. J Dent Res. 2000. 79:77–84.

7. Avellan NL, Sorsa T, Tervahartiala T, Mäntylä P, Forster C, Kemppainen P. Painful tooth stimulation elevates matrix metalloproteinase-8 levels locally in human gingival crevicular fluid. J Dent Res. 2005. 84:335–339.

8. Awawdeh LA, Lundy FT, Linden GJ, Shaw C, Kennedy JG, Lamey PJ. Quantitative analysis of substance P, neurokinin A and calcitonin gene-related peptide in gingival crevicular fluid associated with painful human teeth. Eur J Oral Sci. 2002. 110:185–191.

9. Wahlgren J, Salo T, Teronen O, Luoto H, Sorsa T, Tjäderhane L. Matrix metalloproteinase-8 (MMP-8) in pulpal and periapical inflammation and periapical rootcanal exudates. Int Endod J. 2002. 35:897–904.

10. Figueredo CM, Areas A, Miranda LA, Fischer RG, Gustafsson A. The short-term effectiveness of non-surgical treatment in reducing protease activity in gingival crevicular fluid from chronic periodontitis patients. J Clin Periodontol. 2004. 31:615–619.

11. Apajalahti S, Sorsa T, Railavo S, Ingman T. The in vivo levels of matrix metalloproteinase-1 and -8 in gingival crevicular fluid during initial orthodontic tooth movement. J Dent Res. 2003. 82:1018–1022.

12. Emingil G, Afacan B, Tervahartiala T, Töz H, Atilla G, Sorsa T. Gingival crevicular fluid and serum matrix metalloproteinase-8 and tissue inhibitor of matrix metalloproteinase-1 levels in renal transplant patients undergoing different immunosuppressive therapy. J Clin Periodontol. 2008. 35:221–229.

13. Ingman T, Tervahartiala T, Ding Y, Tschesche H, Haerian A, Kinane DF, Konttinen YT, Sorsa T. Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients. J Clin Periodontol. 1996. 23:1127–1132.

14. Mäntylä P, Stenman M, Kinane DF, Tikanoja S, Luoto H, Salo T, Sorsa T. Gingival crevicular fluid collagenase-2 (MMP-8) test stick for chair-side monitoring of periodontitis. J Periodontal Res. 2003. 38:436–439.

15. Okuda K, Miyazaki A, Momose M, Murata M, Nomura T, Kubota T, Wolff LF, Yoshie H. Levels of tissue inhibitor of metalloproteinases-1 and matrix metalloproteinases-1 and -8 in gingival crevicular fluid following treatment with enamel matrix derivative (EMDOGAIN). J Periodontal Res. 2001. 36:309–316.

16. Passoja A, Ylipalosaari M, Tervonen T, Raunio T, Knuuttila M. Matrix metalloproteinase-8 concentration in shallow crevices associated with the extent of periodontal disease. J Clin Periodontol. 2008. 35:1027–1031.

17. Munjal SK, Prescher N, Struck F, Sorsa T, Maier K, Netuschil L. Evaluation of immunoassay-based MMP-8 detection in gingival crevicular fluid on a point-of-care platform. Ann N Y Acad Sci. 2007. 1098:490–492.

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