Real-time measurement of dentinal fluid flow during desensitizing agent application

Sun-Young Kim; Eun-Joo Kim; In-Bog Lee

doi:10.5395/JKACD.2010.35.5.313

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Kim, Kim, and Lee: Real-time measurement of dentinal fluid flow during desensitizing agent application

Basic Research

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2010; 35(5): 313-320.

Published online: 30 September 2010

DOI: https://doi.org/10.5395/JKACD.2010.35.5.313

Real-time measurement of dentinal fluid flow during desensitizing agent application

Sun-Young Kim, DDS, PhD¹, Eun-Joo Kim, DDS, PhD², In-Bog Lee, DDS, PhD³

Assistant Professor

Fellow

Assistant Professor

¹Department of Conservative Dentistry, KyungHee University School of Dentistry, Seoul, Korea.

²Department of Dental Hygiene, Dong-Nam Health University, Suwon, Korea.

³Department of Conservative Dentistry, Seoul National University School of Dentistry, Seoul, Korea.

Correspondence to In-Bog Lee, DDS, PhD. Associate professor, Department of Conservative Dentistry, Seoul National University School of Dentistry and Dental Research Institute, 275-1 Yeongeon-dong, Jongno-gu, Seoul, Korea 110-768. Tel, +82-2-2072-3953; Fax, +82-2-2072-3859; inboglee@snu.ac.kr

Received 23 April 2010 Revised 23 April 2010 Accepted 27 April 2010

Abstract

Objectives

The aim of this study was to examine changes in the dentinal fluid flow (DFF) during desensitizing agent application and to compare permeability after application among the agents.

Materials and Methods

A Class 5 cavity was prepared to exposure cervical dentin on an extracted human premolar which was connected to a sub-nanoliter fluid flow measuring device (NFMD) under 20 cm water pressure. DFF was measured from before application of desensitizing agent (Seal&Protect, SP; SuperSeal, SS; BisBlock, BB; Gluma desensitizer, GL; Bi-Fluoride 12, BF) through application procedure to 5 min after application.

Results

DFF rate after each desensitizing agent application was significantly reduced when compared to initial DFF rate before application (p < 0.05). SP showed a greater reduction in DFF rate than GL and BF did (p < 0.05). SS and BB showed a greater reduction in DFF rate than BF did (p < 0.05).

Conclusions

Characteristic DFF aspect of each desensitizing agent was shown in NFMD during the application procedure.

Keywords: Dentin hypersensitivity, Dentinal fluid flow, Desensitizing agent, Fluid flow measuring device, Permeability

Figures and Tables

Figure 1

(a) Schematic diagram of the sub-nanoliter scaled dentinal fluid flow measurement system connected to a specimen. (b) Specimen preparation.

Figure 2

A representative curve of consecutive dentinal fluid flow (DFF) during Bi-Fluoride 12 application. Upward (positive slope) movement vs time on graph indicates outward DFF, whereas downward (negative slope) movement indicates inward DFF. A, application of desensitizing agent; d, air dry.

Figure 3

A representative curve of consecutive DFF during Gluma desensitizer application. Upward (positive slope) movement vs time on graph indicates outward DFF, whereas downward (negative slope) movement indicates inward DFF. A, application of desensitizing agent; d, air dry; r, rinse.

Figure 4

A representative curve of consecutive DFF during BisBlock application. Upward (positive slope) movement vs time on graph indicates outward DFF, whereas downward (negative slope) movement indicates inward DFF. A, application of desensitizing agent; r, rinse.

Figure 5

A representative curve of consecutive DFF during SuperSeal application. Upward (positive slope) movement vs time on graph indicates outward DFF, whereas downward (negative slope) movement indicates inward DFF. A, application of desensitizing agent; ga, gentle air dry.

Figure 6

A representative curve of consecutive DFF during Seal&Protect application. Upward (positive slope) movement vs time on graph indicates outward DFF, whereas downward (negative slope) movement indicates inward DFF. A, application of desensitizing agent; LC, light-curing.

Figure 7

Reduction in dentinal fluid flow of desensitizing agents (%). Desensitizing agents under same bar did not show statistically significant difference.

Table 1

Components and application procedures of desensitizing agents used in this study

Abbreviations: PENTA, dipentaerythritol penta acrylate mono monophosphate; HEMA, Hydroxyethylmethacrylate;E&R, acid etching and rinse.

^aE&R were omitted in this study because it was already performed during specimen preparation.

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