Young-Hwan Oh; Young-Joo Jang; Yong-Bum Cho

doi:10.5395/JKACD.2009.34.5.415

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Oh, Jang, and Cho: A bioactivity study of Portland cement mixed with β-glycerophosphosphate on human pulp cell

Original Article

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2009; 34(5): 415-423.

Published online: 30 September 2009

DOI: https://doi.org/10.5395/JKACD.2009.34.5.415

A bioactivity study of Portland cement mixed with β-glycerophosphosphate on human pulp cell

Young-Hwan Oh¹, Young-Joo Jang², Yong-Bum Cho¹

¹Department of Conservative Dentistry, College of Dentistry, Dankook University, Korea.

²Department of Dental Biochemistry, College of Dentistry, Dankook University, Korea.

Corresponding author: Yong-Bum Cho. Department of Conservative Dentistry, College of Dentistry, Dankook University, San 7-1, Shinbu-dong, Cheonan, 330-716, Korea. Tel: 82-41-550-1966, Fax: 82-41-550-1963, raindrop@dku.edu

Received 14 April 2009 Revised 24 April 2009 Accepted 7 August 2009

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study is to investigate the response of human pulp cell on Portland cement mixed with β-glycerophosphate. To investigate the effect of β-glycerophosphate and/or dexamethasone on human pulp cell, ALP activity on various concentration of β-glycerophosphate and dexamethasone was measured and mineral nodule of human pulp cell was stained with Alizarin red S. MTS assay and ALP activity of human pulp cell on Portland cement mixed with various concentration of β-glycerophosphate (10 mM, 100mM, 1M) was measured and the specimens were examined under SEM.

Addition of β-glycerophosphate or dexamethasone alone had no effect however, the addition of 5 mM β-glycerophosphate and 100 nM dexamethasone had the largest increasement in ALP activity. There was no toxicity in all samples and the data showed that Portland cement mixed with 10 mM β-glycerophosphate had more increase in ALP activity compared with control.

In conclusion, Portland cement mixed with β-glycerophosphate has no toxicity and promotes differentiation and mineralization of pulp cell compared with additive-free Portland cement. This implicated that application of Portland cement mixed with β-glycerophosphate might form more reparative dentin and in turn it would bring direct pulp capping to success.

Keywords: Portland cement, β-glycerophosphate, human pulp cell, ALP activity, mineralization

Figures and Tables

Figure 1

Pulp cell emerged from pulp tissue

Figure 2

Indirect contact culture

Figure 3

Insert placed in 24 well

Figure 4

Direct contact culture

Figure 5

Hitachi S-3000H

Figure 6

ALP activity (addition of one differentiated agent alone)

Figure 7

ALP activity (addition of two differentiated agents)

Figure 8

Alizarin red staining (addition of differentiated agents)

Figure 9

MTS assay of pulp cell on Portland cement mixed with β-glycerophosphate

Figure 10

ALP activity of pulp cell on Portland cement mixed with β-glycerophosphate

Figure 11

SEM evaluation of pulp cell on Portland cement mixed with β-glycerophosphate at 1 day. A: Portland cement with no β-glycerophosphate. B, C, D: Portland cement with β-glycerophosphate 10 mM, 100 mM, 1 M.

Figure 12

SEM evaluation of pulp cell on Portland cement mixed with β-glycerophosphate at 3 days. A: Portland cement with no β-glycerophosphate. B, C, D: Portland cement with β-glycerophosphate 10 mM, 100 mM, 1 M.

Figure 13

SEM evaluation of pulp cell on Portland cement mixed with β-glycerophosphate at 7 days. A: Portland cement with no β-glycerophosphate. B, C, D: Portland cement with β-glycerophosphate 10 mM, 100 mM, 1 M.

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