The effect of concentration and application time of hydrogen peroxide on the microtensile bond strength of resin restorations to the dentin at different depths

Jeong-Lyong Son; Gye-Young Lee; Yu-Mi Kang; Young-Taek Oh; Kwang-Won Lee; Tae-Gun Kim

doi:10.5395/JKACD.2009.34.5.406

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Son, Lee, Kang, Oh, Lee, and Kim: The effect of concentration and application time of hydrogen peroxide on the microtensile bond strength of resin restorations to the dentin at different depths

Original Article

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2009; 34(5): 406-414.

Published online: 30 September 2009

DOI: https://doi.org/10.5395/JKACD.2009.34.5.406

The effect of concentration and application time of hydrogen peroxide on the microtensile bond strength of resin restorations to the dentin at different depths

Jeong-Lyong Son, Gye-Young Lee, Yu-Mi Kang, Young-Taek Oh, Kwang-Won Lee, Tae-Gun Kim

Department of Conservative Dentistry, School of Dentistry, Chonbuk National University, Korea.

Corresponding Author: Tae-Gun Kim. Department of Conservative Dentistry, School of Dentistry, Chonbuk National University, Geumam-Dong, Deokjin-Gu, Jeonju, Jeonbuk, 561-712, Korea. Tel: 82-63-250-2319, Fax: 82-63-250-2049, banana994404@daum.net

Received 18 June 2009 Revised 16 July 2009 Accepted 2 September 2009

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study was to examine the effect of hydrogen peroxide at different application time and concentrations on the microtensile bond strength of resin restorations to the deep and the pulp chamber dentin.

A conventional endodontic access cavity was prepared in each tooth, and then the teeth were randomly divided into 1 control group and 4 experimental groups as follows: Group 1, non treated; Group 2, with 20% Hydrogen peroxide(H₂O₂); Group 3, with 10% H₂O₂; Group 4, with 5% H₂O₂; Group 5, with 2.5% H₂O₂; the teeth of all groups except group 1 were treated for 20, 10, and 5min. The treated teeth were filled using a Superbond C&B (Sun medical Co., Shiga, Japan). Thereafter, the specimens were stored in distilled water at 37℃ for 24-hours and then sectioned into the deep and the chamber dentin. The microtensile bond strength values of each group were analyzed by 3-way ANOVA and Tukey post hoc test(p < 0.05).

In this study, the microtensile bond strength of the deep dentin (D1) was significantly greater than that of the pulp chamber dentin (D2) in the all groups tested. The average of microtensile bond strength was decreased as the concentration and the application time of H₂O₂ were increased. Analysis showed significant correlation effect not only between the depth of the dentin and the concentration of H₂O₂ but also between the concentration of H₂O₂ and the application time(p < 0.05), while no significant difference existed among these three variables(p > 0.05). The higher H₂O₂ concentration, the more opened dentinal tubules under a scanning electron microscope(SEM) examination.

Keywords: Hydrogen peroxide, Microtensile bond strength, Deep dentin, Pulp chamber dentin, Oxidizing action, Free radical mechanism, Superbond C&B

Figures and Tables

Figure 1

Schematic presentation of the disc preparation at the deep and the pulp chamber dentin

Figure 2

The manufacture of specimen

Figure 3

Schematic measurement of the microtensile bond strength

Figure 4

Control group(2K). Most surface was covered with smear layer and had scratches (black arrow) due to using a diamond bur. A. D1, B. D2, Bar=20µm.

Figure 5

With 20% H₂O₂ for 20min(2K). Dentinal tubules (white arrow head) were opened completely and the smear layer scarcely remained. A. D1, B. D2, Bar=20µm.

Figure 6

With 10% H₂O₂ for 20min(2K). Dentinal tubules (white arrow head) were opened in a part and the smear layer remained. A. D1, B. D2, Bar=20µm.

Figure 7

With 5% H₂O₂ for 20min(2K). A. D1, Dentinal tubules (white arrow head) were obscure and covered with the smear layer; B. D2, Dentinal tubules were clear and opened in a part. Bar=20µm.

Figure 8

With 2.5% H₂O₂ for 20min(2K). Dentinal tubules (white arrow head) were obscure and covered with the smear layer. A: D1; B: D2; the surface of D1 and D2 had a similar pattern; Bar=20µm.

Table 1

Averages of the microtensile bond strength to the deep and the pulp chamber dentin by H₂O₂ concentration and application time(Mean±SD).

Table 2

3-way ANOVA results showing the relations among the depth of dentin, the concentration and the application time of H₂O₂

D: Depth, C: Concentrations, A: Application times

^*: significant, NS: not significant

Table 3

Interfacial, Resin and Dentin failures in D1.

Table 4

Interfacial, Resin and Dentin failures in D2.

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