Journal List > J Korean Acad Conserv Dent > v.34(1) > 1056357

Yi, Roh, Shin, Lee, Gong, and Lee: THE DYNAMIC CHANGE OF ARTIFICIALLY DEMINERALIZED ENAMEL BY DEGREE OF SATURATION OF REMINERALIZATION SOLUTION AT pH 4.3

Abstract

The purpose of this study is to observe and compare the dynamic change of artificially demineralized enamel by remineralization solutions of different degrees of saturation at pH 4.3.
In this study, 30 enamel specimens were demineralized artificially by lactic acid buffered solution. Each of 10 specimens was immersed in pH 4.3 remineralization solution of three different degrees of saturation (0.22, 0.30, 0.35) for 10 days. After demineralization and remineralization, images were taken by a polarizing microscope (× 100). The density of lesion were determined from images taken after demineralization and remineralization.
During remineralization process, mineral deposition and mineral loss occurred at the same time. After remineralization, total mineral amount and width of surface lesion increased in all groups. The higher degree of saturation was, the more mineral deposition occurred in surface lesion and the amount of mineral deposition was not much in subsurface lesion. Total demineralized depth increased in all groups.

References

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Figure 1.
Enamel specimen used in the experiment
jkacd-34-20f1.tif
Figure 2.
Polarizing microscopic observation of demineralized enamel (Group1, × 100)
jkacd-34-20f2.tif
Figure 3.
Polarizing microscopic observation of remineralized enamel (Group1, × 100)
jkacd-34-20f3.tif
Figure 4.
Polarizing microscopic observation of demineralized enamel (Group 2, × 100)
jkacd-34-20f4.tif
Figure 5.
Polarizing microscopic observation of remineralized enamel (Group 2, × 100)
jkacd-34-20f5.tif
Figure 6.
Polarizing microscopic observation of demineralized enamel (Group 3, × 100)
jkacd-34-20f6.tif
Figure 7.
Polarizing microscopic observation of remineralized enamel (Group 3, × 100)
jkacd-34-20f7.tif
Figure 8.
Rate of change of surface lesion width at Group 1, 2, 3 ((width of surface lesion after remineralization / width of surface lesion before remineralization) × 100(%)). Horizontal bars represent no statistically significant differences (P > .05).
jkacd-34-20f8.tif
Figure 9.
Comparison of mineral density in enamel before and after remineralization (Group 1)
jkacd-34-20f9.tif
Figure 10.
Comparison of mineral density in enamel before and after remineralization (Group 2)
jkacd-34-20f10.tif
Figure 11.
Comparison of mineral density in enamel before and after remineralization (Group 3)
jkacd-34-20f11.tif
Table 1.
Initial composition of demineralization solution
Composition Concentration
Lactic acid (mM) 100
Calcium (mM) 15.5
Phosphate (mM) 8.5
Sodium azide (mM) 3.08
pH 4.3
Table 2.
Initial composition of remineralization solution
Composition Group
1 2 3
Lactic acid (mM) 10.00 10.00 10.00
Calcium (mM) 19.98 27.56 33.17
Phosphate (mM) 7.94 10.25 12.52
Sodium azide (mM) 3.08 3.08 3.08
Fluoride (ppm) 2.00 2.00 2.00
pH 4.30 4.30 4.30
Degree of saturation 0.22 0.30 0.35
Table 3.
Rate of change of quantitative value during demineralization & remineralization at enamel
Condition Demineralized Surface lesion Mineral
Depth (%)
Width (%)
Change (%)
Group (Mean ±S.D.) (Mean ±S.D.) ( (Mean ±S.D.)
1 115.1 ±11.4 % jkacd-34-20-t3.tif* 109.8 ±3.8 %
2 112.6 ±7.9 % 101.9 ±2.2 %
3 111.4 ±6.5 % 102.7 ±4.7 %

* P < .05

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