The remineralizing features of pH 5.5 solutions of different degree of saturations on artificially demineralized enamel

Young-Jun Kwak; Eui-seoug Kim; Sung-Ho Park; Hyung Kyu Gong; Yoon Lee; Chan-Young Lee

doi:10.5395/JKACD.2008.33.5.481

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Kwak, Kim, Park, Gong, Lee, and Lee: The remineralizing features of pH 5.5 solutions of different degree of saturations on artificially demineralized enamel

Original Article

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2008; 33(5): 481-492.

Published online: 30 September 2008

DOI: https://doi.org/10.5395/JKACD.2008.33.5.481

The remineralizing features of pH 5.5 solutions of different degree of saturations on artificially demineralized enamel

Young-Jun Kwak, Eui-seoug Kim, Sung-Ho Park, Hyung Kyu Gong, Yoon Lee, Chan-Young Lee

Department of Conservative Dentistry, Yonsei University, Seoul, Korea.

Corresponding Author: Chan-Young Lee. Department of Conservative Dentistry, College of Dentistry, Yonsei University, 134, Shinchon-dong, Seodaemun-gu, Seoul, 120-752, Korea. Tel: 82-2-2228-8700, chanyoungl@yuhs.ac

Received 12 August 2008 Revised 22 August 2008 Accepted 2 September 2008

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study is to observe and compare the remineralization tendencies of artificially demineralized enamel by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel).

For this study, 36 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 3 days in order to produce dental caries artificially that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffered remineralization solution of three different degrees of saturation (0.25, 0.30, 0.35) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope (× 100). The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were determined.

In conclusion, in the group with the lowest degree of saturation, remineralization occurred thoroughly from the surface to the subsurface lesion, whereas in the groups with greater degree of saturation showed no significant change in the subsurface lesion, although there was corresponding increase in the remineralization width on the surface zones.

Keywords: Demineralization, Remineralization, pH, Degree of saturation

Figures and Tables

Figure 1

Specimen used in the experiment.

Figure 2

Polarizing microscopic observation of demineralized enamel (Group 1, × 100).

Figure 3

Polarizing microscopic observation of remineralized enamel (Group 1, × 100).

Figure 4

Polarizing microscopic observation of demineralized enamel (Group 2, × 100).

Figure 5

Polarizing microscopic observation of remineralized enamel (Group 2, × 100).

Figure 6

Polarizing microscopic observation of demineralized enamel (Group 3, × 100).

Figure 7

Polarizing microscopic observation of remineralized enamel (Group 3, × 100).

Figure 8

Change rate of demineralized depth (demineralized depth after remineralization / demineralized depth before remineralization) × 100 (%).

Figure 9

Change rate of surface lesion width (surface lesion width after remineralization / surface lesion width before remineralization) × 100 (%).

Figure 10

Comparison of density in enamel area before and after remineralization (Group 1).

Figure 11

Comparison of density in enamel area before and after remineralization (Group 2).

Figure 12

Comparison of density in enamel area before and after remineralization (Group 3).

Figure 13

Change rate of mineral density before and after remineralization ((demineralized area before remineralization - demineralized area after remineralization / demineralized area before remineralization) × 100 + 100 (%))

Table 1

Initial composition of demineralization solution

Table 2

Initial composition of remineralization solution

Table 3

One-way ANOVA on the remineralizing features of pH 5.5 solutions of different degree of saturations on artificially demineralized enamel

^*p < .05

Table 4

Quantitative value change (%) and the post-hoc result of enamel during de- and remineralization

Standard Deviation (SD) is in the parentheses.

^*p < .05

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