Abstract
The purpose of this study was to compare the apical microleakage in root canal filled with Resilon by several self-etching primers and methacrylate-based root canal sealer. Seventy single-rooted human teeth were used in this study. The canals were instrumented by a crown-down manner with Gate-Glidden drills and .04 Taper Profile to ISO #40. The teeth were randomly divided into four experimental groups of 15 teeth each according to root canal filling material and self-etching primers and two control groups (positive and negative) of 5 teeth each as follows: group 1 - gutta percha and AH26® sealer; group 2 - Resilon, RealSeal™ primer and RealSeal™ sealer; group 3 - Resilon, Clearfil SE Bond® primer and RealSeal™ sealer group 4 - Resilon, AdheSe® primer and RealSeal™ sealer. Apical leakage was measured by a maximum length of linear dye penetration of roots sectioned longitudinally by diamond disk. Statistical analysis was performed using the One-way ANOVA followed by Scheffe's test. There were no statistical differences in the mean apical dye penetration among the groups 2, 3 and 4 of self-etching primers. And group 1, 2 and 3 had also no statistical difference in apical dye penetration. But, there was statistical difference between group 1 and 4 (p < 0.05). The group 1 showed the least dye penetration. According to the results of this study, Resilon with self-etching primer was not sealed root canal better than gutta precha with AH26® at sealing root canals. And there was no significant difference in apical leakage among the three self-etching primers.
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References
1. Sundqvist G, Figdor D, Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998. 85:86–93.


2. Dow PR, Ingle JI. Isotope determination of root canal failure. Oral Surg Oral Med Oral Pathol. 1955. 8:1100–1104.


3. Naidorf IJ. Clinical microbiology in endodontics. Dent Clin North Am. 1974. 18:329–344.
4. Torabinejard M, Ung B, Dettering JD. In vitro bacterial penetration of coronally unsealed endodontically treated teeth. J Endod. 1990. 16:566–569.
5. Gish SP, Drake DR, Walton RE, Wilcox L, Coronal leakage. bacterial penetration through obturated canals following post preparation. J Am Dent Assoc. 1994. 125:1369–1372.


6. Khayat A, Lee SJ, Torabinejad M. Human saliva penetration of coronally unsealed obturated root canals. J Endod. 1993. 19:458–461.


7. Gerosa R, Menegazzi G, Borin M, Cavalleri G. Cytotoxicity evaluation of six root canal sealers. J Endod. 1995. 21:446–448.


8. Kazemi RB, Safavi KE, Spångberg LS. Dimensional cahanges of endodontic sealers. Oral Surg Oral Med Oral Pathol. 1993. 76:766–771.
9. Imai Y, Komabayashi T. Properties of a new injectable type of root canal filling resin with adhesiveness to dentin. J Endod. 2003. 29:20–23.


10. Leonard JE, Gutmann JL, Guo IY. Apical and coronal seal of roots obturated with a dentine bonding agent and resin. Int Endod J. 1996. 29:76–83.


11. Lee MJ, Park SH, Choi GW. Comparison of apical seal with or without the use of dentin adhesive system. J Korean Acad Conserv Dent. 2005. 30(1):7–15.


12. Shipper G, Ørstavik D, Teixeira FB, Trope M. An evaluation of microbial leakage in roots filled with a thermoplastic synthetic polymer-based root canal filling material (Resilon). J Endod. 2004. 30:342–347.


13. Sybron Dental Specialties Inc.SybronEndo, RealSeal™, Frequently Asked Questions. Available from: URL: http://www.sybronendo.com/index/sybronendo-fill-real-seal-faq-02.
14. Teixeira FB, Teixeira EC, Thompson J, Leinfelder KF, Trope M. Dentinal bonding reaches the root canal system. J Esthet Restor Dent. 2004. 16:348–354.


15. Teixeira FB, Teixeira EC, Thompson JY, Trope M. Fracture resistance of roots endodontically treated with a new resin filling material. J Am Dent Assoc. 2004. 135:646–652.


16. Shin SJ, Lee Y, Park JW. Evaluation of retrievability using a new soft resin based root canal filling material. J Korean Acad Conserv Dent. 2006. 31(4):323–329.


17. Yamada M, Miyazaki M, Onose H, Moore BK. Influence of interchanging self-etching primers and bonding agents on bond strengths to bovine dentin. Am J Dent. 2005. 18:155–159.
18. Tagger M, Tagger E, Tjan AH, Bakland LK. Measurement of adhesion of endodontic sealers to dentin. J Endod. 2002. 28:351–354.


19. Gettleman BH, Messer HH, Eldeeb ME. Adhesion of sealer cements to dentin with and without the smear layer. J Endod. 1991. 17:15–20.


20. Duke ES. Technology may significantly improve endodontic therapy. Compend Contin Educ Dent. 2005. 26:408410–411.
21. Kardon BP, Kuttler S, Hardigan P, Dorn SO. An in vitro evaluation of the sealing ability of a new rootcanal obturation system. J Endod. 2003. 29:658–661.


22. Shipper G, Trope M. In vitro microbial leakage of endodontically trated teeth using new and standard obturation techniques. J Endod. 2004. 30:154–158.


23. Sevimay S, Kalayci A. Evaluation of apical sealing ability and adaptation to dentine of two resin-based sealers. J Oral Rehabil. 2005. 32:105–110.


24. Van Meerbeek B, Perdigão J, Lambrechts P, Vanherle G. The clinical performance of adhesives. J Dent. 1998. 26:1–20.


25. Perdigão J, Lopes M. Dentin bonding - Questions for the new millennium. J Adhes Dent. 1999. 1:191–209.
26. Perdigão J. Dentin bonding as a function of dentin structure. Dent Clin North Am. 2002. 46:277–301.


27. Tay FR, Pashley DH. Aggressiveness of contemporary self-etching systems. I Depth of penetration beyond dentin smear layers. Dent Mater. 2001. 17:296–308.
28. Miyazaki M, Onose H, Moore BK. Analysis of the dentin-resin interface by use of laser Raman spectroscopy. Dent Mater. 2002. 18:576–580.


29. Miyazaki M, Hirohata N, Takagaki K, Onose H, Moore BK. Influence of self-etching primer drying time on enamel bond strength of resin composites. J Dent. 1999. 27:203–207.


30. Hotta M, Kondoh K, Kamemizu H. Effect of primers on bonding agent polymerization. J Oral Rehabil. 1998. 25:792–799.


31. Starkey DL, Anderson RW, Pashly DH. An evaluation of the effects of methylene blue dye pH on apical leakage. J Endod. 1993. 19:435–439.
32. Choi JJ, Hong CU. The effect of Microseal obturation technique on the apical seal of root canals. J Korean Acad Conserv Dent. 1999. 24(2):356–363.
33. Haikel Y, Wittenmeyer W, Bateman G, Bentaleb A, Allemann C. A new method for the quantitative analysis of endodontic microleakage. J Endod. 1999. 25:172–177.


34. Chailertvanitkul P, Saunders WP, Mackenzie D. An assessment of microbial coronal leakage in teeth root filled with gutta percha and three different sealers. Int Endod J. 1996. 29:387–392.


35. Jacquot BM, Panighi MM, Steinmetz P, G'Sell C. Evaluation of temporary restorations by means of electrochemical impedance measurements. J Endod. 1996. 22:586–589.


36. Ahlberg KM, Assavanop P, Tay WM. A comparison of the apical dye penetration patterns shown by methylene blue and India ink in root-filled teeth. Int Endod J. 1995. 28:30–34.


37. Wu MK, Wesselink PR. Endodontic leakage studies reconsidered, Part I. Methodology, application and relevance. Int Endod J. 1993. 26:37–43.


38. Shipper G, Teixeira FB, Arnold R, Trope M. Periapical inflammation after coronal microbial inocultation of dog roots filled with gutta-percha or Resilon. J Endod. 2005. 31:91–96.


39. Bodrumlu E, Tunga U. Apical leakage of Resilon obturation material. J Contemp Dent Pract. 2006. 7:45–52.


40. Gogos C, Econnomides N, Stavrianos C, Kolokouris I, Kokorikos I. Adhesion of a new methacrylate resin-based sealer to human dentin. J Endod. 2004. 30:238–240.


41. Tay FR, Loushine RJ, Weller RN, Kimbrough WF, Pashley DH, Mak YF, et al. Ultrasturctural evaluation of the apical seal in roots filled with a polycaprolactone-based root canal filling material. J Endod. 2005. 31:514–519.


42. Hiraishi N, Papacchini F, Loushine RJ, Weller RN, Ferrari M, Pashley DH, et al. Shear bond strength of Resilon to a methacrylate-based root canal sealer. Int Endod J. 2005. 38:753–763.


43. Gesi A, Raffaelli O, Goracci C, Pashley DH, Tay FR, Ferrari M. Interfacial strength of Resilon and gutta-percha to intraradicular dentin. J Endod. 2005. 31:809–813.

