The effect of ethylene glycol analogs on mechanical properties of moist demineralized dentin matrix

Kyung-Ha Lee; Young-Gon Cho; Kwang-Won Lee

doi:10.5395/JKACD.2006.31.4.290

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Lee, Cho, and Lee: The effect of ethylene glycol analogs on mechanical properties of moist demineralized dentin matrix

Original Article

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2006; 31(4): 290-299.

Published online: 31 July 2006

DOI: https://doi.org/10.5395/JKACD.2006.31.4.290

The effect of ethylene glycol analogs on mechanical properties of moist demineralized dentin matrix

Kyung-Ha Lee, Young-Gon Cho¹, Kwang-Won Lee

Department of Conservative Dentistry, School of Dentistry, Chonbuk National University, Korea.

¹Department of Conservative Dentistry, College of Dentistry, Chosun University, Korea.

Corresponding Author: Kwang-Won Lee. Department of Conservative Dentistry, School of Dentistry, Chonbuk National University, 664-14 Duckjin-dong, Duckgin-gu, Jeonju, Korea, 561-756. Tel: +82-63-250-2016, lkw@chonbuk.ac.kr

Received 4 April 2006 Revised 27 April 2006 Accepted 2 May 2006

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives

The purpose of this study is to evaluate the effect of ethylene glycol analogs on modulus of elasticity and ultimate tensile strength of moist, demineralized dentin matrix.

Methods

Dentin disks 0.5 mm thick were prepared from mid-coronal dentin of extracted, unerupted, human third molars. "I" beam and hour-glass shaped specimens were prepared from the disks, the ends protected with nail varnish and the central regions completely demineralized in 0.5M EDTA for 5 days. Ultimate tensile stress (UTS) and low strain modulus of elasticity (E) were determined with specimens immersed for 60 min in distilled water (H₂O), ethylene glycol (HO-CH₂-CH₂-OH), 2-methoxyethanol (H₃CO-CH₂-CH₂-OH), and 1,2-dimethoxyethane (H₃CO-CH₂-CH₃-OCH₃) prior to testing in those same media. Modulus of elasticity was measured on the same specimens in a repeated measures experimental design. The results were analyzed with a one-way ANOVA on ranks, followed by Dunn's test at α = 0.05. Regression analysis examined the relationship between UTS or E and hoy's solubility parameter for hydrogen bonding (δ_h) of each solvent.

Results

The UTS of demineralized dentin in water, ethylene glycol, 2-methoxyethanol, and 1,2-dimethoxyethane was 24 (3), 30 (5), 37 (6), and 45 (6) MPa, × (SD) N = 10. Low strain E for the same media were 16 (13), 23 (14), 52 (24), and 62 (22) MPa. Regression analysis of UTS vs δ_h revealed a significant (p < 0.0001, r = -0.99, R² = 0.98) inverse, exponential relationship. A similar inverse relationship was obtained between low strain E vs δ_h (p < 0.0005, r = -0.93, R² = 0.86).

Significance

The tensile properties of demineralized dentin are dependent upon the hydrogen bonding ability of polar solvents (δ_h). Solvents with low δ_h values may permit new interpeptide H-bonding in collagen that increases its tensile properties. Solvents with high δ_h values prevent the development of these new interpeptide H-bonds.

Keywords: Polar Solvents, Ethylene Glycol Analogs, Hydrogen Bonding Ability

Figures and Tables

Figure 1

Schematic of sample preparation from mid-coronal dentin disk 0.5 mm thick (A). Hour-glass (B) and "I" beam (C) specimens were cut from the discs.

Figure 2

Two dimensional chemical structures of the control and test solvents.

Figure 3

Illustration of how the demineralized "I" beam was placed into friction grips of a universal testing machine.

Figure 4

Split-aluminum mold to measure the ultimate tensile strength by placing hourglass pattern into depressions.

Figure 5

UTS Values of Polar Solvents in 0.5 M EDTA Demineralized Dentin.

Figure 6

Hoy's Solubility Parameter vs UTS of Demineralized Dentin Matrix.

Figure 7

Hoy's Solubility Parameter vs UTS of Demineralized Dentin Matrix.

Figure 8

Low Wtrain Modulus of Elasticity of Polar Solvents.

Figure 9

Hoy's Solubility Parameter vs Low Strain Modulus of Elasticity.

Table 1

Hoy's solubility parameters, molecular weight and concentrations of test solvents

hydrogen bonding forces (δ_h), dispersive forces (δ_d), polar forces (δ_p), Hildebrand's solubility parameter (δ_t). MV=molecular weight(g/mole)

Table 2

Solvent exposure order to the demineralized dentin

Table 3

Summary of the effects of polar solvents on the tensile properties of demineralized dentin

a UTS of specimens demineralized with 0.5 M EDTA.

b UTS of specimens demineralized with 37% H₃PO₄.

^*5% strain modulus of elasticity.

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