Expression of OD314 during ameloblast differentiation and maturation

Joo-Cheol Park; Seong-Min Ahn; Heung-Joong Kim; Moon-Jin Jeong; Min-Ju Park; In-Cheol Shin; Ho-Hyun Son

doi:10.5395/JKACD.2005.30.5.423

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Park, Ahn, Kim, Jeong, Park, Shin, and Son: Expression of OD314 during ameloblast differentiation and maturation

Original Article

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2005; 30(5): 423-431.

Published online: 30 September 2005

DOI: https://doi.org/10.5395/JKACD.2005.30.5.423

Expression of OD314 during ameloblast differentiation and maturation

Joo-Cheol Park¹, Seong-Min Ahn¹, Heung-Joong Kim¹, Moon-Jin Jeong¹, Min-Ju Park¹, In-Cheol Shin¹, Ho-Hyun Son²

¹Department of Oral Histology and BK21, School of Dentistry, Chosun University, Korea.

²Department of Conservative Dentistry, School of Dentistry, Seoul National University, Korea.

Corresponding author: Ho-Hyun Son. Department of Conservative Dentistry, School of Dentistry, Seoul National University, 28 Yongon-dong, Chongno-gu, Seoul, Korea 110-749. Tel: 82-2-2072-2652, Fax: 82-2-2072-3859, hhson@snu.ac.kr

Received 13 May 2005 Revised 28 July 2005 Accepted 23 August 2005

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ameloblasts are responsible for the formation and maintenance of enamel which is an epithelially derived protective covering for teeth. Ameloblast differentiation is controlled by sequential epithelial-mesenchymal interactions. However, little is known about the differentiation and maturation mechanisms. OD314 was firstly identified from odontoblasts by subtraction between odontoblast/pulp cells and osteoblast/dental papilla cells, even though OD314 protein was also expressed in ameloblast during tooth formation.

In this study, to better understand the biological function of OD314 during amelogenesis, we examined expression of the OD314 mRNA and protein in various stages of ameloblast differentiation using in-situ hybridization and immunohistochemistry.

The results were as follows :

1. The ameloblast showed 4 main morphological and functional stages referred to as the presecretory, secretory, smooth-ended, and ruffle-ended.

2. OD314 mRNA was expressed in secretory ameloblast and increased according to the maturation of the cells.

3. OD314 protein was not expressed in presecretory ameloblast but expressed in secretory ameloblast and maturative ameloblast. OD314 protein was distributed in entire cytoplasm of secretory ameloblast. However, OD314 was localized at the proxiamal and distal portion of the cytoplasm of smooth-ended and ruffle-ended ameloblast.

These results suggest that OD314 may play important roles in the ameloblast differentiation and maturation.

Keywords: Ameloblast, OD314, Differentiation, Maturation, Smooth-ended, Ruffle-ended

Figures and Tables

Figure 1

The various functional stages of the ameloblast. Panel A, Ameloblast of presecretory stage; panel B, Ameloblast of secretory stage; panel C, Smooth-ended ameloblast of maturative stage; panel D, Ruffle-ended ameloblast of maturative stage.

Figure 2

Mandibular incisor of 3-week-old mouse hybridized with antisense cRNA probes of OD314. Panel A, Low magnification micrograph showing OD314 mRNA expression; panel B, High power view of zone I in panel A; panel C, High power view of zone II in panel A; panel D, High power view of zone III in panel A.

Figure 3

Immunohistochemical localization of OD314 in the various functional stages of ameloblast. Panel A, Ameloblast of presecretory stage; panel B, Ameloblast of secretory stage; panel C, Smooth-ended ameloblast of maturative stage; panel D, Ruffle-ended ameloblast of maturative stage.

References

1. Garant PR. Oral cells and tissues. 2003. Chicago, IL: Quintessence Publishing Co., Inc.;25–52.

2. Nancy A. Ten Cate's oral histology: development, structure, and function. 2003. 6th ed. St. Louis, Missouri: Mosby, Inc.;192–239.

3. Bei M, Stowell S, Maas R. Msx2 controls ameloblast terminal differentiation. Dev Dyn. 2004. 231:758–765.

4. Millar SE, Koyama E, Reddy ST, Andl T, Gaddapara T, Piddington R, Gibson CW. Over- and ectopic expression of Wnt3 causes progressive loss of ameloblasts in postnatal mouse incisor teeth. Connect Tissue Res. 2003. 44:124–129.

5. Smith CE. Cellular and chemical events during enamel formation. Crit Rev Oral Biol Med. 1998. 9:128–161.

6. Wang XP, Suomalainen M, Jorgez CJ, Matzuk MM, Werner S, Thesleff I. Follistatin regulates enamel patterning in mouse incisors by asymetrically inhibitting BMP signalling and ameloblast differentiation. Dev Cell. 2004. 7:719–730.

7. Dey R, Son HH, Cho MI. Isolation and partial sequencing of potentially odontolast-specific/enriched rat cDNA clones obtained by suppression subtractive hybridization. Arch Oral Biol. 2001. 46:249–260.

8. Kim DH, Kim HJ, Jeong MJ, Son HH, Park JC. Expression and functional characterization of odontoblast-derived gene: OD314. J Korean Acad Conserv Dent. 2004. 29(4):399–408.

9. Kim HJ, Jeong MJ, Son HH, Park JC. Inactivation of the OD314 Gene by RNA Interference in Preodontoblast Cell Lines. Korean J Phys Anthropol. 2004. 17(2):121–129.

10. Kim IH. Role of OD314 During Odontoblast Differentiation. 2005. Chosun University;Doctor's Degree Dissertation.

11. Couble ML, Farges JC, Bleicher F, Perrt-Mabillon B, Boudeulle M, Maloire H. Odontoblast differentiation of human dental pulp cell in explant culture. Calcif Tissue Int. 2000. 66:129–138.

12. D'souza RN, Cavender A, Sunavala G, Alvarez J, Ohshima T, Kulkarni AB, Macdougall M. Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialiphosphoprotein (DSPP) suggest distinct developmental functions in vivo. J Bone Miner Res. 1997. 12:2040–2049.

13. Gaikwad GS, Hoffman M, Cavender A, Bronskers AL, D'Souza RN. Molecular insights into the lineage-specific determenation on odontoblasts: the role of CBFA1. Adv Dent Res. 2001. 15:19–24.

14. Buchaille R, Couble ML, Magloire H, Bleicher F. A substractive PCR-based cDNA library from human odontoblast cells: identification of novel gene expressed in tooth forming cells. Matrix Biol. 2000. 19:421–430.

15. Nakashima K, Zhou X, Kunkel G, Zhang Z, Deng JM, Behringer RR, Crombrugghe B. The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell. 2002. 108:17–29.

16. Butler WT. Dentin matrix protein. Eur J Oral Sci. 1998. 106:Suppl 1. 204–210.

17. Butler WT. Dentin-specific protein. Methods Enzymol. 1987. 145:290–303.

18. Butler WT, Bhown M, Brunn JC, D'souza RN, Farachcarson MC, Hartha RP, Schrohenloher RE, Seyer JM, Somerman MJ, Foster RA, Tomana M, Djik SV. Isolation, charaterization immunolocalization of a 53-KDal dentin sialoprotein (DSP). Matrix. 1992. 12:343–351.

19. MacDougall M, Simmons D, Luan X, Nydegger J, Feng J, Gu TT. Dentin phosphoprotein and dentin sialoprotein are cleavage products expressed from a single transcript coded by a gene on human chromosome 4. J Biol Chem. 1997. 272(2):835–842.

20. Papagerakis P, Berdal A, Mesbah M, Peuchmaur M, Malaval L, Nydegger J, Simmer J, Macdougall M. Investigation of osteocalcin, osteonectin, and dentin sialophosphoprotein in developing human teeth. Bone. 2002. 30(2):377–385.

21. Shimo T, Wu C, Billings PC, Piddington R, Rosenbloom J, Pacifici M, Koyama E. Expression, gene regulation, and roles of Fisp 12/CTGF in developing tooth germs. Dev Dyn. 2002. 224(3):267–278.

22. Sreenath T, Thyagarajan T, Hall B, Longenecker G, D'Souza R, Hong S, Wright JT, MacDougall M, Sauk J, Kulkarni AB. Dentin sialophosphoprotein knockout mouse teeth display widened predentin zone and develop defective dentin mineralization similar to human dentonogenesis imperfecta type III. J Biol Chem. 2003. 278(27):24874–24880.

23. Calvi LM, Shin HI, Knight MC, Weber JM, Young MF, Giovannetti A, Schipani E. Constitutively active PTH/PTHrP receptor in odontoblasts alters odontoblast and ameloblast function and maturation. Mech Dev. 2004. 121:397–408.

24. Kawamoto T, Shimozu M. Pathway and speed of calcium movement from blood to mineralizing enamel. J Histochem Cytochem. 1997. 45:213–230.

25. Kukita A, Harada H, Kukita T, Inai T, Matsuhashi S, Kurisu K. Primary and secondary culture of rat ameloblasts in serum-free medium. Calcif Tissue Int. 1992. 51:393–398.

26. Chen LS, Couwenhoven RI, Hsu D, Luo W, Snead ML. Maintenance of amelogenin gene expression by transformed epithelial cells of mouse enamel organ. Arch Oral Biol. 1992. 37:771–778.

27. Nakata A, Kameda T, Nagai H, Ikegami K, Duan Y, Terada K, Sugiyama T. Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line. Biochem Biophys Res Commun. 2003. 308:834–839.

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