Isolation and identification of bacteria from the root canal of the teeth diagnosed as the acute pulpitis and acute periapical abscess

Yeon-Jae Lee; Mi-Kwang Kim; Ho-Keel Hwang; Joong-Ki Kook

doi:10.5395/JKACD.2005.30.5.409

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Lee, Kim, Hwang, and Kook: Isolation and identification of bacteria from the root canal of the teeth diagnosed as the acute pulpitis and acute periapical abscess

Original Article

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2005; 30(5): 409-422.

Published online: 30 September 2005

DOI: https://doi.org/10.5395/JKACD.2005.30.5.409

Isolation and identification of bacteria from the root canal of the teeth diagnosed as the acute pulpitis and acute periapical abscess

Yeon-Jae Lee¹, Mi-Kwang Kim², Ho-Keel Hwang^1,³, Joong-Ki Kook^2,³

¹Department of Conservative Dentistry, College of Dentistry, Chosun University, Korea.

²Department of Oral Biochemistry, College of Dentistry, Chosun University, Korea.

³Oral Biology Research Institute, College of Dentistry, Chosun University, Korea.

Corresponding author: Joong-Ki Kook. Department of Oral Biochemistry, and Oral Biology Research Institute, College of Dentistry, Chosun University, 375 Seo-suk Dong, Dong-Gu, Gwang-ju, 501-759 Korea. Tel: 82-62-230-6877, Fax: 82-62-224-3706, jkkook@chosun.ac.kr

Received 11 April 2005 Revised 16 May 2005 Accepted 13 June 2005

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to identify the bacteria isolated from acute endodontic lesions by cell culture and 16S rDNA sequencing. The necrotic pulpal tissue was collected from 17 infected root canals, which were diagnosed as being either an acute pulpitis or acute periapical abscess. Samples were collected aseptically from the infected pulpal tissue of the infected root canals using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing 500 ul of 1 X PBS. The sample solution was briefly mixed and plated onto a BHI-agar plate containing 5% sheep blood. The agar plates were incubated in a 37℃ anaerobic chamber for 7 days. The bacteria growing on the agar plate were identified by 16S rRNA coding gene (rDNA) cloning and sequencing at the species level. Among the 71 colonies grown on the agar plates, 56 strains survived and were identified. In dental caries involving the root canals, Streptococcus spp. were mainly isolated. Actinomyces, Clostridia, Bacteroides and Fusobacteria were isolated in the periapical lesion without dental caries. Interestingly, two new Actinomyces spp. (ChDC B639 and ChDC B631) were isolated in this study. These results showed that there was diversity among the species in endodontic lesions. This suggests that an endodontic infection is a mixed infection with a polymicrobial etiology. These results may offer the bacterial strains for pathogenesis studies related to an endodontic infection.

Keywords: Acute pulpitis, Acute periapical abscess, Bacteria, 16S rRNA gene

Figures and Tables

Table 1

Clinical features of the patients

Table 2

Bacteria isolated from each of patient

ChDC; Department of Oral Biochemistry, College of Dentistry, Chosun University

Table 3

Bacteria isolated from necrotized pulpal tissue

ChDC; Department of Oral Biochemistry, College of Dentistry, Chosun University

^*; GenBank accession number

Table 4

Summary of the isolated clones derived from endodontic lesions

Table 5

Comparison of the bacteria isolated from the endodontic infection according to the presence of caries in infected teeth

Table 6

Comparison of the bacteria isolated from the endodontic infection according to the presence of the apical lesion

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