The effect of canal filling with gutta-percha or resilon on Enterococcus faecalis in bovine dentinal tubules

Sang-Wook Jee; Euiseong Kim; Il-Young Jung; Yun-Jung Yoo

doi:10.5395/JKACD.2005.30.5.385

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Jee, Kim, Jung, and Yoo: The effect of canal filling with gutta-percha or resilon on Enterococcus faecalis in bovine dentinal tubules

Original Article

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2005; 30(5): 385-392.

Published online: 30 September 2005

DOI: https://doi.org/10.5395/JKACD.2005.30.5.385

The effect of canal filling with gutta-percha or resilon on Enterococcus faecalis in bovine dentinal tubules

Sang-Wook Jee¹, Euiseong Kim¹, Il-Young Jung¹, Yun-Jung Yoo²

¹Department of Conservative Dentistry, Yonsei University, Korea.

²Department of Oral Biology, College of Dentistry, Yonsei University, Korea.

Corresponding author: Euiseong Kim. Department of Conservative Dentistry, College of Dentistry, Yonsei University, Sinchon-dong 134, Seodaemun-gu, Seoul, Korea 120-752. Tel: 82-2-2228-8700, andyendo@yumc.yonsei.ac.kr

Received 22 February 2005 Revised 24 May 2005 Accepted 13 June 2005

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study was to observe the effect of canal filling on the bacteria left in the dentinal tubules and to compare the sealing ability between Gutta-percha and Resilon. The bovine dentin block models were prepared. E. faecalis was inoculated to dentin blocks and incubated. The dentin blocks were divided into 5 groups.

Group 1 was the negative control. Group 2 was the positive control. Group 3 was filled with ZOE based sealer and Gutta-percha, Group 4 with resin based sealer and Gutta-percha, and Group 5 with resin based sealer and Resilon. After 24 hour, the blocks were incubated at 37℃ for 1, 2, 3 and 4 weeks on BHI agar plates.

The internal dentin portion of the blocks was removed using ISO 027, 029, 031, 035 round burs and the dentin chips were incubated at 37℃ for 24 hour. Following incubation, the optical density of the medium was measured. The data were statistically analysed using repeated measures ANOVA and one-way ANOVA.

The results were as follows,

1. There was statistically significant reduction in the number of E. faecalis of the group where dentinal tubules were completely sealed with nail varnish in comparison with the groups obturated with gutta-percha or resilon (p < 0.05).

2. In group 5, the number of E. faecalis in the dentinal tubules decreased significantly with time (p < 0.05), whereas in Group 3 and 4, there was no reduction in its number (p > 0.05).

3. Under the conditions of this experiment, E. faecalis survived up to 4 weeks after obturation with gutta-percha or resilon (p > 0.05).

Keywords: Enterococcus faecalis, Resilon, Root canal sealing effect

Figures and Tables

Figure 1

Dentin disk block.

Figure 2

Schematic illustration of incubating dentin block on BHI agar.

Figure 3

The change of optical density with time.

^*significant difference statistically (p < 0.05).

Table 1

Experimental groups by canal filling materials (n = 32)

Table 2

E. faecalis left in dentinal tubules

Data were expressed as optical density of culture media of dentin chip.

Data description form: mean (s.d.).

^*: p-value by repeated measures ANOVA with time.

^†: statistically significant by repeated measures ANOVA.

^**: p-value by one-way ANOVA among groups at given experimental time.

a, b: multiple comparison using tukey method.

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