A study on the hemolytic properties of Prevotella nigrescens

Ju-Seok Kwak; Hoon-Sang Jang; Seok-Woo Jang; Su-Jong Lee; Yong-Wook Yu; Kyung-San Min

doi:10.5395/JKACD.2005.30.4.335

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Kwak, Jang, Jang, Lee, Yu, and Min: A study on the hemolytic properties of Prevotella nigrescens

Original Article

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2005; 30(4): 335-343.

Published online: 30 July 2005

DOI: https://doi.org/10.5395/JKACD.2005.30.4.335

A study on the hemolytic properties of Prevotella nigrescens

Ju-Seok Kwak¹, Hoon-Sang Jang¹, Seok-Woo Jang¹, Su-Jong Lee¹, Yong-Wook Yu², Kyung-San Min¹

¹Department of Conservative Dentistry, College of Dentistry, Wonkwang University, Korea.

²Department of Oral Biochemistry, College of Dentistry, Wonkwang University, Korea.

Corresponding author: Kyung-San Min. Instructor, Department of Conservative Dentistry, College of Dentistry, Wonkwang University, 344-2 Shinyong-dong, Iksan, Cheonbuk, Korea, 570-749. Tel: 82-63-850-6930, Fax: 83-63-850-1932, mksdd@wonkwang.ac.kr

Received 12 January 2005 Revised 18 February 2005 Accepted 21 February 2005

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Hemolytic property is a specific feature of bacteria to obtain iron which is essential for its survival in host tissues. Therefore, it is thought to be one of several factors of virulence. The purpose of this study was to investigate the hemolytic properties of Prevotella nigrescens isolated from the teeth diagnosed as pulp necrosis and apical periodontitis under the presence of hemolysin inhibitors such as NaN₃ and dithiothreitol, heat, various pH and cultural conditions.

The results were as follows;

1. Clinically isolated P. nigrescens strains and standard P. nigrscens ATCC 33563 showed hemolytic activity.

2. P. nigrescens showed higher hemolytic activity against human erythrocytes than sheep or horse erythrocytes.

3. NaN₃ and dithiothreitol (DTT) reduced the hemolytic activity of P. nigrescens in a dose dependent manner (p < 0.05).

4. Optimal pH for the maximum hemolytic activity of P. nigrescens was 4.0 and the hemolysin was stable under the 50℃, but the hemolytic activity was significantly decreased at 95℃.

5. P. nigrescens cultured in 10% CO₂ condition showed higher hemolytic activity than the bacteria cultured in the anaerobic condition.

Keywords: P. nigrescens, Hemolytic property, Iron, Virulence, Erythrocyte, Hemolysin inhibitor

Figures and Tables

Table 1

Hemolytic activity of P. nigrescens isolates

Table 2

Hemolytic activity of P. nigrescens ATCC 33563 and P. nigrescens 2304 strains based on erythrocytes

^aRelative hemolytic activity (%)

^bND; not detected

Table 3

Effect of NaN3 on hemolytic activity of P. nigrescens ATCC 33563 and P. nigrescens 2304

^aRelative hemolytic activity (%)

^bND; not detected

^*p < 0.05 compared with control by Mann-Whitney U test

Table 4

Effect of dithiothreitol (DTT) on hemolytic activity of P. nigrescens ATCC 33563 and P. nigrescens 2304

^aRelative hemolytic activity (%)

^bND; not detected

^*p < 0.05 compared with control by Mann-Whitney U test

Table 5

Effect of pH on hemolytic activity of P. nigrescens ATCC33563 and 2304

^aND; not detected

Table 6

Effect of temperature on hemolytic activity of P. nigrescens ATCC 33563 and P. nigrescens 2304

^aND; not detected

Table 7

Effect of various culture status on hemolytic activity of P. nigrescens ATCC 33563 and P. nigrescens 2304

^aND ; not detected

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