Cytotoxicity of resin-based root canal sealer, adseal

Hee-Jung Kim; Seung-Ho Baek; Woo-Cheol Lee; Han-Soo Park; Kwang-Shik Bae

doi:10.5395/JKACD.2004.29.6.498

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Kim, Baek, Lee, Park, and Bae: Cytotoxicity of resin-based root canal sealer, adseal

Original Article

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2004; 29(6): 498-503.

Published online: 30 November 2004

DOI: https://doi.org/10.5395/JKACD.2004.29.6.498

Cytotoxicity of resin-based root canal sealer, adseal

Hee-Jung Kim, Seung-Ho Baek, Woo-Cheol Lee, Han-Soo Park, Kwang-Shik Bae

Department of Conservative Dentistry, College of Dentistry, Seoul National University, Korea.

Corresponding author: Kwang-Shik Bae. Department of Conservative Dentistry, College of Dentistry, Seoul National University, 28 Yoengun-dong, Chongro-gu, Seoul, Korea, 110-749. Tel: 82-2-2072-2650, Fax: 82-2-2072-3859, baeks@plaza.snu.ac.kr

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The properties of ideal root canal sealers include the ability of sealing the total root canal system and no toxic effects to periradicular tissues. Cytotoxicity test using cell culture is a common screening method for evaluation of the biocompatibility of root canal sealers. The purpose of this study was to investigate the cytotoxic effect of newly developed resin-based sealer (Adseal 1, 2, and 3) comparing with those commercial resin-based sealers (AH26 and AH Plus), ZOE-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT) and calcium hydroxide based sealer (Sealapex). An indirect contact test of cytotoxicity by agar diffusion was performed according to the international standard ISO 10993-5. L929 fibroblast cells were incubated at 37℃ in humidified 5% CO₂-containing air atmosphere. The freshly mixed test materials were inserted into glass rings of internal diameter 5 mm and height 5 mm placed on the agar. After the 24 hrs incubation period, the decolorization zones around the test materials were assessed using an inverted microscope with a calibrated screen. A Decolorization Index was determined for each specimen. Adseal 1, 2, and 3 did not exert any cytotoxic effects, whereas AH26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, and Sealapex produced mild cytotoxicity.

Keywords: Cytotoxicity, Agar diffusion test, Root canal sealer, Decolorization index, Adseal

Figures and Tables

Figure 1

Decolorization zone and cell appearance by agar diffusion test (inverted microscope, left: × 200, right: × 400). Each sealer has two pictures. The left sides show the decolorization zone except Adseal 1, 2, and 3. Adseal 1, 2, and 3 show normal cell morphology and cell density.

Table 1

Compositions of Adseal 1, 2, and 3

^*Adseal 1 and Adseal 3 have same ingredients but they are different in the ratio of ingredients.

Table 2

Criteria for scoring of decolorization index

Table 3

Decolorization index of experimental sealers (agar diffusion test)

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