Journal List > J Korean Acad Conserv Dent > v.29(2) > 1056098

Kang, Cho, Kim, Park, Yoo, Jin, Choi, and Ki: Effect of rewetting agent on dentinal microtensile bond strength

Abstract

This study investigated that the effect of rewetting agent on dentinal microtensile bond strength (µTBS). Human molars were sectioned to expose the superficial dentin surfaces.
Samples were divided into two groups according to type of adhesives-Single Bond (S) and One-Step (O)], and again subdivided into five groups by different dentin surface treatment-dry for 15s (D), blot dry (BD) or dry for 15s, and rewet with different rewetting agents [distilled water (DW), Gluma Desensitizer (GD) and Aqua-Prep (AP)] for 30s. After application of adhesive, composite resin was built up on the bonding surface. Each tooth was sectioned to obtain stick with 1 mm2 cross sectional area and the µTBS was determined by EZ test.
In the S group, the mean µTBS of GD, AP and BD group was significantly higher than that of DW and D group (p < 0.05). In the O group, the mean µTBS of AP, GD, BD and DW group was significantly higher than that of D group (p < 0.05).
The data suggested that Gluma Desensitizer and Aqua-Prep could be successfully used as rewetting agents, and Distilled water could be acceptable in aceton based adhesive system only.

Figures and Tables

Figure 1
Specimen adhered to the testing apparatus
jkacd-29-153-g001
Figure 2
EZ test for microtensile bond strength test
jkacd-29-153-g002
Figure 3
Microtensile bond strength (MPa) of each group according to adhesives and dentin surface treatment
jkacd-29-153-g003
Table 1
Chemical formulations of the adhesive systems used
jkacd-29-153-i001

Bis-GMA = bisphenol-glysidyl-methacrylate, HEMA = 2-hydroxyethyl methacrylate, BPDM = biphenyl dimethacrylate

Table 2
Chemical formulations of the rewetting agent used
jkacd-29-153-i002
Table 3
The mean microtensile bond strength (MPa) and statistical analysis of each adhesive in different dentin surface treatment
jkacd-29-153-i003

S: Single Bond, O: One-Step

Superscripts of the same letter indicate values of no statistical significant difference (p > 0.05).

Table 4
The mean microtensile bond strength (MPa) according to different dentin treatment and statistical analysis
jkacd-29-153-i004

Superscripts of the same letter indicate values of no statistical significant difference (p > 0.05).

References

1. Amaral CM, Hara AT, Pimenta LAF, Rodrigues AL Jr. Microleakage of hydrophillic systems in class V composite restorations. Am J Dent. 2001. 14(1):31–34.
2. Nakajima M, Sano H, Urabe I. Bond strength of single bottle dentin adhesives to caries-affected dentin. Oper Dent. 2000. 25:2–10.
3. Tay FR, Gwinnett AJ, Wei SHY. Micromorphological spectrum from overdrying to overwetting acid-conditioned dentin in water-free, acetone-based, single-bottle primer-adhesives. Dent Mater. 1996. 12:236–244.
crossref
4. Nakajima M, Kanemura N, Pereira PNR, Tagami J, Pashley DH. Comparative microtensile bond strength and SEM analysis of bonding to wet and dry dentin. Am J Dent. 2000. 13(6):324–328.
5. Perdigão J, Swift EJ, Heymann HO, Malek MA. Effect of a re-wetting agent on the performance of acetone-based dentin adhesives. Am J Dent. 1998. 11:207–213.
6. Perdigão J, Frankenberger R. Effect of solvent and rewetting time on dentin adhesion. Quintessence Int. 2001. 32(5):385–390.
7. Gwinnett AJ. Moist versus dry dentin: its effect on shear bond strength. Am J Dent. 1992. 5:127–129.
8. Kanca J. Effect of drying on bonding strength. J Dent Res. 1991. 70:304. (Abstract 1029).
9. Gwinnett AJ. Dentin bond strength after air drying and rewetting. Am J Dent. 1994. 7(3):144–148.
10. Marshall GW, Watanabe LG, Wu-Magidi IL. AFM study of drying and rehydration of etched dentin. J Dent Res. 1996. 75:390.
11. Tay FR, Gwinnett AJ, Wei SHY. Ultrastructure of the resin-dentin interface following reversible and irreversible re-wetting. Am J Dent. 1997. 10:77–82.
12. Perdigao J, Van Meerbeek B, Lopes MM, Ambrose WW. The effect of a re-wetting agent on dentin bonding. Dent Mater. 1999. 15:282–295.
crossref
13. Ritter AV, Heymann HO, Swift EJ Jr, Perdigao J, Rosa BT. Effects of different re-wetting techniques on dentin shear bond strengths. J Esthet Dent. 2000. 12(2):85–96.
crossref
14. Pashley DH, Carvalho RM, Sano H, Ciucchi B, Nakajima M, Yoshiyama M, Shono T, Fernades CA, Tay F. The micro-tensile bond test: a review. J Adhes Dent. 1999. 1:299–309.
15. Erickson RL, Glasspoole EA. Bonding to tooth structure: a comparison of glass-ionomer and composite-resin systems. J Esthet Dent. 1994. 6:227–244.
crossref
16. Chappell RP, Schreiner RF, Glaros AG, Eixk J. Pilot study of determine sample size for micro-tensile testing. J Dent Res. 1997. 76:38. (Abstract 193).
17. Sano H, Shono T, Sonoda H, Takatsu T, Ciccuchi B, Carvalho R, Pashley DH. Relationship between surface area for adhesion and tensile bond strength-Evaluation of a microtensile bond test. J Dent Res. 1995. 74:1093–1102.
18. Schreiner RF, Chappell RP, Glaros AG, Erick JD. Microtensile testing of dental adhesives. Dent Mater. 1998. 14(3):194–201.
19. Sano H, Shono T, Sonoda H, Takatsu T, Ciccuchi B, Horner JA, Phashley DH. Tensile bond strength versus surface area for dentin bonding-Evaluation of a method using limited surface areas. J Conserv Dent. 1994. 37:882–887.
20. Perinka L, Sano H, Hosoda H. Dentin thickness, hardness, and Ca-concentration vs bond strength of dentin adhesives. Dent Mater. 1992. 8:229–233.
crossref
21. Cho YG, Park YY, Lee YG. Effect of a rewetting agent on dentin adhesion. J Korean Acad Conserv Dent. 2003. 28(1):11–22.
crossref
22. Nakabayashi N, Kojima K, Masuhara E. The promotion of adhesion by infiltration of monomers into tooth substrates. J Biomed Mater Res. 1982. 16:265–273.
crossref
23. Pioch T, Staehle HJ, Schneider H, Duschner H, Dorfer CE. Effect of intrapulpal pressure simulation in vitro on shear bond strenghs and hybrid layer formation. Am J Dent. 2001. 14(5):319–323.
24. Eick JD, Robinson SJ, Chappell RP, Cobb CM, Spencer P. The dentinal surface: its influence on dentinal adhesion. Part III. Quintessence Int. 1993. 24(8):571–582.
25. Pashley DH, Sano H, Ciucchi B, Yoshiyama M, Carvalho RM. Adhesion testing of dentin bonding agents: a review. Dent Mater. 1995. 11:117–125.
crossref
26. Perdigão J, Geraldeli S, Carmo ARP, Dutra HR. In vivo influence of residual moisture on microtensile bond strength of one-bottle adhesives. J Esthet Restor Dent. 2002. 14(1):31–38.
crossref
27. Van Meerbeek B, De Munck J, Yoshida Y, Inoue S, Vargas P, Vijay P, Van Landuyt K, Lambrechts P, Vanherle G. Adhesion to enamel and dentin: Current status and future challenges. Oper Dent. 2003. 28:215–235.
28. Suzuke K, Takahashi M, Nakai H. Treatment of dentin by aqueous solution of amino acid derivatives: HEMA. J Adhes Dent. 1990. 8:43–51.
29. Cardoso PE, Braga RR, Carrilho MRO. Evaluation of micro-tensile, shear and tensile tests determining the bond strengths of three adhesive systems. Dent Mater. 1998. 14:394–398.
crossref
30. Kwong SM, Cheung GSP, Kei LH, Itthagatun A, Smales RJ, Tay FR. Micro-tensile bond strengths to sclerotic dentin using a self-etching and a total-etching technique. Dent Mater. 2002. 18:359–369.
crossref
31. Nunes MF, Swift EJ, Perdigao J. Effects of adhesive composition on microtensile bond strength to humam dentin. Am J Dent. 2001. 14:340–343.
32. Phrukkanon S, Burrow MF, Tyas M. The influence of cross-sectional shape and surface area on the microtensile bond test. Dent Mater. 1998. 14:212–222.
crossref
33. Kanca J. Effect of resin primer solvents and surface wetness on resin composite bond strength to dentin. Am J Dent. 1992. 5:213–215.
34. Kanca J. Wet bonding: Effect of drying time and distance. Am J Dent. 1996. 9:273–276.
35. Carvalho RM, Yoshima M, Pashley EL, Pashley DH. In vitro study on the dimensional changes of human after demineralization. Arch Oral Biol. 1996. 41:369–377.
crossref
36. Tay FR, Gwinnett AJ, Pang KM, Wei SHY. Resin permeation into acid-conditioned, moist, and dry dentin: a paradigm using water free adhesive primers. J Dent Res. 1996. 75:1034–1044.
crossref
37. Gallo JR, Henderson M, Burgess JO. Shear bond strength to moist and dry dentin of four dentin bonding systems. Am J Dent. 2000. 13(5):267–270.
38. Kanca J 3rd. Resin bonding to wet substrate. I. bonding to dentin. Quintessence Int. 1992. 23(1):39–41.
39. Jacobsen T, Soderholm KJ. Effect of primer solvent, primer agitation and dentin dryness on shear bond strength to dentin. Am J Dent. 1998. 11(5):225–228.
40. Maciel KT, Carvalho RM, Ringle RD, Preston CD, Russell CM, Pashley DH. The effects of acetone, ethanol, HEMA, and air on the stiffness of human decalcified dentin matrix. J Dent Res. 1996. 75:1851–1858.
crossref
41. Harashima I, Hirasawa T. Adsorption of 2-hydroxyethyl methacrylate on dentin from aqueous solution. Dent Mater J. 1990. 9:36–46.
crossref
42. Nakabayashi N, Takarada K. Effects of HEMA on bonding to dentin. Dent Mater. 1992. 8:125–130.
43. Nakabayashi N, Watanae A, Gendusa NJ. Dentin adhesion of modified 4-META/MMA-TBB resin: function of HEMA. Dent Mater. 1992. 8:259–264.
crossref
44. Sugizaki J. The effects of various primers on dentin adhesion of resin composites. SEM and TEM observation of the resin impregnated layer and adhesion promoting effect of the primer. Jap J Cons Dent. 1991. 34:228–265.
45. Hansen EK, Asmussen E. Improved efficacy of dentin bonding agents. Eur J Oral Sci. 1997. 105(5 Pt 1):434–439.
TOOLS
Similar articles