The verification of the MTT assay on the viability of periodontal ligamental cells in rat molars through the histologic examination

Hyun-Ki Kim; Eui-Seoung Kim; In-Bok Choi; Jin Kim; Seung-Jong Lee

doi:10.5395/JKACD.2003.28.5.385

Journal List > J Korean Acad Conserv Dent > v.28(5) > 1056072

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Kim, Kim, Choi, Kim, and Lee: The verification of the MTT assay on the viability of periodontal ligamental cells in rat molars through the histologic examination

Original Article

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2003; 28(5): 385-391.

Published online: 30 September 2003

DOI: https://doi.org/10.5395/JKACD.2003.28.5.385

The verification of the MTT assay on the viability of periodontal ligamental cells in rat molars through the histologic examination

Hyun-Ki Kim, Eui-Seoung Kim, In-Bok Choi, Jin Kim, Seung-Jong Lee

Department of Conservative Dentistry, College of dentistry, Yonsei University, Korea.

Corresponding author (sjlee@yumc.yonsei.ac.kr)

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study is to examine the viability of PDL cells in rat molars by using MTT assay and to verify the MTT assay through the histologic observation. Thirty of Sprague-Dawley white female rats of 4-weeks old with a body weight of about 100 grams were used. Groupings are as follows:

Immediate Group : Positive control group(n=10)-after extraction immediately.

Dried Group : Negative control group(n=10)-after drying for an hour under warm dry.

ViaSpan® Group : 1hour ViaSpan® group(n=10)-after storing in ViaSpan® at 4℃ for 1hour.

Ten teeth of each group were treated as same as above and replanted to the original socket of experimental animals. After two weeks of replantation, all the experimental animals were sacrificed. And after fixation, extracted maxillary jaw was dimineralized. After it was embedded in paraffin, serial section by 5µm was carried out and for construction of specimen, hematoxylin-eosin dye was used.

The mean MTT measurement of immediate group(positive control) is 2.81 and the mean measurement of dried group(negative control) is 0.98 which is significant differnt(P<0.05). The mean measurement of ViaSpan® group is 2.65 and there is significant difference between dried group and ViaSpan® group(P<0.05). However, there is no difference between immediate group and ViaSpan® group. The average resorption points of immediate group is 3.03 points. In the dried group, average 6.44 points resorption and 2.68 points showed resorption in the ViaSpan® group. Unlike with MTT assay, there was no significant difference between the immediate group and ViaSpan® group.

The usage of MTT assay as a viable cell marker may give us a better indication of the maintenance of periodontal ligament cell vitality.

Keywords: MTT assay, Root resorption, ViaSpan®

Figures and Tables

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Table 1

Mean and standard deviation of the optical density(OD)

^*Statistically significant at the 95% level of confidence

Table 2

The mean number of resorption points

^*Statistically significant at the 95% level of confidence

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