Identification of putative pathogens in acute endodontic infections by PCR based on 16S rDNA

Jee-Hoon Kim; So Young Yoo; Sun-A Lim; Joong-Ki Kook; Sang-Soo Lim; Seul-Hee Park; Ho-Keel Hwang

doi:10.5395/JKACD.2003.28.2.178

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Kim, Yoo, Lim, Kook, Lim, Park, and Hwang: Identification of putative pathogens in acute endodontic infections by PCR based on 16S rDNA

Original Article

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2003; 28(2): 178-183.

Published online: 31 March 2003

DOI: https://doi.org/10.5395/JKACD.2003.28.2.178

Identification of putative pathogens in acute endodontic infections by PCR based on 16S rDNA

Jee-Hoon Kim¹, So Young Yoo^2,³, Sun-A Lim², Joong-Ki Kook^2,³, Sang-Soo Lim¹, Seul-Hee Park¹, Ho-Keel Hwang^1,³

¹Department of Conservative Dentistry, College of Dentistry, Chosun University, Korea.

²Department of Oral Biochemistry, College of Dentistry, Chosun University, Korea.

³Oral Biology Research Institute, College of Dentistry, Chosun University, Korea.

Corresponding author (rootcanal@hanmail.net)

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study was to investigate the frequency of 7 putative pathogens in endodontic infections. The specimens were collected from infected pulpal tissue of patients who were referred for root canal treatment to the department of conservative dentistry, Chosun University. Samples were collected aseptically using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing 500 ml of 1 X PBS. DNAs were extracted from the samples by direct DNA extraction method using lysis buffer (0.5% EDTA, 1% Triton X-100). Identification of 7 putative pathogens was performed by PCR based on 16S rDNA. The target species were as follows: Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Bacteroides forsythus, Actinobacillus actinomycetemcomitans, and Treponema denticola. Our data revealed that the prevalence of P. endodontalis was found in 88.6% (39/54), P. gingivalis 52.3% (23/44), P. nigrescens 18.2% (8/44), P. intermedia 15.9% (7/44), B. forsythus 18.2% (8/44), A. actinomycetemcomitans 2.3% (1/44), T. denticola 25% (11/44) of the samples. The high prevalence of P. endodontalis and P. gingivalis suggests that they may play an important role in the etiology of endodontic infections.

Keywords: 16S rDNA, Acute endodontic infections, PCR, Pathogens

Figures and Tables

Table 1

PCR primer pairs used for detection of putative oral pathogens in samples

Table 2

Prevalence of some bacterial species in infected root canals

Pe ; Porphylomonas endodontalis, Pg ; Porphylomonas ginginvalis, Pn ; Prevotella nigrescens, Pi ; Prevotella intermedia, Td ; Treponema denticola, Bf ; Bacteroide forsythus, Aa ; Actinobacillus actinomycetemcomitans ^*n ; Sample number

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