Effect of Sodium Silicate on Hair Growth in C57BL/6 Mice

Jin-Joo Hue; Bum-Ki Jo; Bong Su Kang; Jun-hyeong Kim; Sang Yoon Nam; Young Won Yun; Jong Soo Kim; Jae-Hwang Jeong; Sang-Hwa Lee; Jun Bae Ahn; Beom Jun Lee

doi:10.5625/lar.2010.26.1.55

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Hue, Jo, Kang, Kim, Nam, Yun, Kim, Jeong, Lee, Ahn, and Lee: Effect of Sodium Silicate on Hair Growth in C57BL/6 Mice

Original Article

Lab. Anim. Res. 2010;26(1):55-62.

Published online: 17 March 2010

DOI: https://doi.org/10.5625/lar.2010.26.1.55

Effect of Sodium Silicate on Hair Growth in C57BL/6 Mice

Jin-Joo Hue¹, Bum-Ki Jo¹, Bong Su Kang¹, Jun-hyeong Kim¹, Sang Yoon Nam¹, Young Won Yun¹, Jong Soo Kim¹, Jae-Hwang Jeong², Sang-Hwa Lee³, Jun Bae Ahn⁴, Beom Jun Lee^1,^∗

¹College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, Korea

²Department of Biotechnology and Biomedicine, Chungbuk Province College, Okcheon, Koea

³Department of Food and Nutrition, Seowon University, Chungju, Korea

⁴Department of Food Service Industry, Seowon University, Chungju, Korea

^∗Corresponding author: Beom Jun Lee, Department of Veterinary Public Health, College of Veterinary Medicine, Chungbuk National University, 48 Gaeshin-dong, Heungduk-gu, Cheongju 361-763, Korea Tel: +82-43-261-3357 Fax: +82-43-271-3246 E-mail: beomjun@cbu.ac.kr

Received 24 March 2010 Revised 10 March 2010 Accepted 13 March 2010

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A promoting effect of Na²SiO³ on hair regrowth was investigated using an animal model of C57BL/6 mice. There were four experimental groups including distilled water (DW, a negative control), 5% minoxidil (MXD, a positive control), 50% Na²SiO^3, and 100% Na²SiO³ solution. The animals were shaved with an electric clipper and then test solutions applied daily with a volume of 0.2 ml per to the dorsal skin of mice for 3 weeks. Body weight and food and water consumption were measured weekly. Photographs of hair regrowth were taken at experimental day 0, 4, 7, 10, 14, 17, and 21. Activities of alkaline phosphatase and γ-glutamyl transpeptidase as well as expressions of growth factors were also determined in the dorsal skin of mice. The animal body weight were not significantly changed among the experimental groups. The MXD and Na²SiO³ accelerated hair regrowth compared with DW. The elongation of hair follicles were evidently observed in MXD and 50 or 100% Na²SiO³ groups. MXD significantly increased γ-glutamyl transpeptidase at day 14, compared with DW (P<0.05). But the activities of alkaline phosphatase and γ-glutamyl transpeptidase were not significantly increased in Na²SiO³ groups, compared with DW. The expression of epidermal growth factor was significantly increased in MXD and Na²SiO³ groups, compared with DW (P<0.05). The expression of vascular endothelial growth factor was not significantly changed by MXD or Na²SiO³ treatments. The expression of transforming growth factor (TGF)-β1 was clearly decreased in MXD and Na²SiO³ groups, compared with DW. These results indicate that Na²SiO³ may have a hair growth-promoting activity and it can be used for treatment of alopecia or boldness in humans.

Keywords: Na²SiO³, hair growth, ALP, γ, -GT, EGF, VEGF, minoxidil

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Figure 1.

Change in body weight of C57BL/6 mice for 3 weeks. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. D.W: distilled water, MXD: 5% minoxidil. Date represent mean±SD (n=5).

Figure 2.

Photograph of hair regrowth in C57BL/6 mice. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. The photographs were taken day 0, 4, 7, 10, 14, 17, and 21. DW: distilled water, MXD: 5% minoxidil.

Figure 3.

Histological change of hair follicles in the dorsal skin of C57BL/6 mice (H&E, ×40). The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. D.W: distilled water, MXD: 5% minoxidil.

Figure 4.

Alkaline phosphatase (ALP) changes in the dorsal tissue of C57BL/6mice. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. D.W.: Distilled water, MXD: Minoxidil. Data represent mean±SD (n=5).

Figure 5.

γ-glutamyl transpeptidase (γ-GT) changes in the dorsal tissue of C57BL/6 mice. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. DW: Distilled water, MXD: Minoxidil. Data represent mean±SD (n=5). ∗Significantly different from the D.W. group (P<0.05).

Figure 6.

Activities of Endothelial growth factor (EGF) in the dorsal tissue of C57BL/6 mice. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. D.W.: Distilled water, MXD: 5% Minoxidil. Data represent mean±SD (n=5). ∗Significantly different from the D.W. group (P<0.05).

Figure 7.

Activities of vascular endothelial growth factor (VEGF) in the dorsal tissue of C57BL/6 mice. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. D.W.: Distilled water, MXD: 5% Minoxidil. Data represent mean±SD (n=5).

Figure 8.

Expression of TGF-β in the dorsal skin of C57BL/6 mice. The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. D.W.: Distilled water, MXD: 5% Minoxidil.

Table 1.

Comparison of hair regrowth in C57BL/6 mice for 3 weeks

Weeks	Groups
Weeks	D.W.	MXD	50% Na²SiO³	100% Na²SiO³
1	-	-	-	-
2	-	++	±	+
3	+	+++	+++	+++

The animals were shaved with electric clipper and the test compounds applied with 0.2 mL per to the dorsal skin of mice for 3 weeks. Hair growth was evaluated by the scoring index: 0-19% (-), 20-39% (±), 40-59% (+), 60-79% (++), 80-100% (+++). D.W: distilled water, MXD: 5% minoxidil.

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