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Park, Lee, Bae, Choi, Kim, Yang, Yang, Kwon, Lee, Joo, and Kim: Antioxidative and Antidiarrheal Effects of Persimmon Extracts
Original Article

Lab. Anim. Res. 2010;26(4):407-413.

Published online: 20 January 2010

DOI: https://doi.org/10.5625/lar.2010.26.4.407

Antioxidative and Antidiarrheal Effects of Persimmon Extracts

Dongsun Park1, Sun Hee Lee1, Dae-Kwon Bae1, Young Jin Choi1, Tea Kyun Kim1, Yun-Hui Yang1, Goeun Yang1, Sang-Chul Kwon2, Do Ik Lee3, Sung Soo Joo4, Yun-Bae Kim1, ∗

1College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea

2Chamsunjin Total Food Co., Ltd., Jincheon, Korea

3College of Pharmacy, Chung-Ang University, Seoul, Korea

4Division of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung, Gangwon, Korea

∗Corresponding authors: Sung Soo Joo, Division of Marine Molecular Biotechnology, Gangneung-Wonju National University, 120 Gangneungdaehakro, Gangneung, Gangwon 210-702, Korea Tel: +82-33-640-2856 Fax: +82-33-640-2340 E-mail: larryjoo@hanmail.net

Yun-Bae Kim, College of Veterinary Medicine, Chungbuk National University, 410 Seongbongro (Gaesin-dong), Cheongju, Chungbuk 361-763, Korea Tel: +82-43-261-3358 Fax: +82-43-271-3246 E-mail: solar93@cbu.ac.kr

Received 17 November 2010       Revised 1 December 2010       Accepted 5 December 2010

Copyright © 2010 Korean Association for Laboratory Animal Science

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Since oxidative stresses are involved in gastroenteritis and diarrhea, we investigated antioxidative and antidiarrheal activities of persimmon flesh extract (PFE) and persimmon calyx extract (PCE) in vitro and in vivo, respectively. PCE significantly scavenged 1,1-diphenyl-2-picrylhydrazyl hydrate and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) from 500 µg/mL, although PFE was ineffective. In addition, PFE and PCE exhibited strong nitric oxide-scavenging effects from 1 µg/mL, in which PCE was superior to ascorbic acid (50 µM). Furthermore, PFE and PCE significantly inhibited FeCl3-induced lipid peroxidation as well as Cu2+/H2O2-induced protein oxidation from 10 µg/mL. In vivo charcoal-propulsion assay, in contrast to a negligible effect of PFE, treatment with PCE (160–500 mg/kg) markedly inhibited intestinal motility. The results indicate that extracts of persimmon, especially PCE, possess antioxidative, antiinflammatory and antidiarrheal activities. Therefore, it is suggested that persimmon extracts could be used for the relief of gastroenteritis and diarrhea.

Keywords: Persimmon, antioxidative activity, antidiarrheal activity, gastrointestinal motility

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Figure 1.
1,1-Diphenyl-2-picrylhydrazyl radical-scavenging activities of persimmon flesh extract (PFE) and persimmon calyx extract (PCE). Asc: ascorbic acid (50 µM). ∗Significantly different from vehicle control (100%) (P<0.05).
lar-26-407f1.tif
Figure 2.
2,2'-Azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS)-scavenging activities of persimmon flesh extract (PFE) and persimmon calyx extract (PCE). Asc: ascorbic acid (50 µM). ∗Significantly different from vehicle control (100%) (P<0.05).
lar-26-407f2.tif
Figure 3.
Nitric oxide-scavenging activities [% of control (100%)] of persimmon flesh extract (PFE) and persimmon calyx extract (PCE). Asc: ascorbic acid (50 µM).
lar-26-407f3.tif
Figure 4.
Inhibitory effects of persimmon flesh extract (PFE) and persimmon calyx extract (PCE) on FeCl3-induced lipid peroxidation. BHT: butylated hydroxytoluene (10 µM). ∗Significantly different from vehicle control (100%) (P<0.05).
lar-26-407f4.tif
Figure 5.
Inhibitory effects of persimmon flesh extract (PFE) and persimmon calyx extract (PCE) on Cu2+/H2O2-induced protein [bovine serum albumin (BSA)] oxidation. Asc: ascorbic acid (50 µM).
lar-26-407f5.tif
Figure 6.
Inhibitory effects of persimmon flesh extract (PFE) and persimmon calyx extract (PCE) on carbachol (Carb, 0.5 mg/kg)-induced gastrointestinal motility (charcoal propulsion). Asc: ascorbic acid (50 µM). ∗Significantly different from Carb alone (P<0.05).
lar-26-407f6.tif
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