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Jo, Han, Kang, Jang, Kim, Ahn, and Kim: Modifying Effect of Indole-3-carbinol on the Rat Colon Carcinogenesis
Original Article

Lab. Anim. Res. 2010;26(4):339-343.

Published online: 20 January 2010

DOI: https://doi.org/10.5625/lar.2010.26.4.339

Modifying Effect of Indole-3-carbinol on the Rat Colon Carcinogenesis

Hyunmu Jo1, Beom Seok Han2, Jin Seok Kang3, Dong Deuk Jang4, Kisok Kim5, Byeongwoo Ahn6, Dae Joong Kim6, ∗

1Institute of Clinical Medicine Research, Yeouido St' Mary's Hospital, Catholic University of Korea

2Hoseo Toxicological Research Center, Hoseo University Hoseo Toxicological Research Center, Hoseo University

3Department of Biomedical Laboratory Science, Namseoul University

4Daejeon Korea Food and Drug Administration

5Department of Public Health, College of Natural Sciences, Keimyung University

6College of Veterinary Medicine, Chungbuk National University

∗Corresponding author: Dae Joong Kim, College of Veterinary Medicine, Chungbuk National University, 410 Seongbong-ro, Heungduk-gu, Cheongju 361-763, Korea Tel: +82-43-261-3446 Fax: +82-43-271-3246 E-mail: kimdj@cbu.ac.kr

Received 9 November 2010       Accepted 25 November 2010

Copyright © 2010 Korean Association for Laboratory Animal Science

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Indole-3-carbinol (I3C) found in various cruciferous vegetables has been shown to exert anti-carcinogenic activity in several target organs. Our study was conducted to assess the modifying effect of I3C on the development of colon tumor induced by azoxymethane (AOM). Eighty-seven male F344 rats were divided into 5 groups and were treated with AOM followed by I3C 100 or 300 ppm, AOM alone, I3C alone, and non-treatment, respectively. The animals were subcutaneously injected with AOM. Then diet containing I3C were fed to the rats for 37 weeks. All rats were sacrificed at 40 weeks. Liver and kidney weights of rats treated with I3C at doses of 100 or 300 ppm were significantly increased compared to those of the control group. Colonic tumor incidence and multiplicity of rats treated with I3C at doses of 100 and 300 ppm were not significant compared to those of AOM alone group. In the pathological examination, most of tumors were classified with adenoma and adenocarcinoma in the small and large intestine. These results demonstrated that I3C may have not chemopreventive effect on the rat colon carcinogenesis.

Keywords: Indole-3-carbinol (I3C), colon carcinogenesis, azoxymethane (AOM), rat

References

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Figure 1.
Experimental design.
lar-26-339f1.tif
Table 1.
Final body weight and organ weights of rats
Treatments No. of Rats Final body weight (g) Liver (g) Kidney (g)
AOM alone 28 0382.4±35.3a 9.4±0.9 2.2±0.2
AOM→I3C 100 23 401.2±42.4 10.3±0.70 ∗2.4±0.2∗
AOM→I3C 300 26 392.0±37.7 10.4±0.70 2.4±0.2
I3C alone 05 426.3±37.7 12.0±0.9∗ 2.5±0.2
Normal 05 407.2±7.20 9.9±0.3 2.4±0.1

∗ : Significantly different from the values of AOM alone group or normal group at P<0.05 with Dunnett's t-test.

a : Values represent the mean±SD.

Table 2.
Effect of I3C on the tumor incidence and multiplicity in the small intestine induced by AOM
Treatments No. of Rats Hp AD ADC Total
Incidence Incidence Multiplicity Incidence Multiplicity Incidence Multiplicity
AOM alone 28 0(0) 1(3.5) 0.04±0.2a. 6(21) 0.2±0.4 7(25) 0.3±0.4
AOM→I3C 100 23 0(0) 3(13) 0.1±0.3 5(22) 0.3±0.4 7(30) 0.3±0.6
AOM→I3C 300 26 0(0) 1(3.8) 0.0±0.2 5(19) 0.3±0.7 6(23) 0.3±0.7
I3C alone 05 0(0) 0(0) – 0(0) – 0(0) –
Non-treatment 05 0(0) 0(0) – 0(0) – 0(0) –

Hp: Hyperplasia, AD: Adenoma, ADC: Adenocarcinoma. Parenthesis represents the percentage of the tumor incidence.

a : Values represent mean±SD.

Table 3.
Effect of I3C on the tumor incidence and multiplicity in the large intestine induced by AOM
Treatment No. of Rat   Hp AD ADC Total
Incidence Multiplicity Incidence Multiplicity Incidence Multiplicity Incidence Multiplicity
AOM alone 28 1(4) 00.03±0.2a 24(86) 2.01±1.4 6(21) 0.22±0.4 25(89) 2.23±1.50
AOM→I3C 100 23 5(22) 0.22±0.4 20(87) 1.34±0.9 5(22) 0.34±0.5 22(96) 1.83±0.90
AOM→I3C 300 26 3(12) 0.21±0.6 22(84) 1.71±1.4 8(31) 0.33±0.5 24(92) 2.1±1.4
I3C alone 05 0(0) – 0(0) – 0(0) – 0(0) –
Normal 05 0(0) – 0(0) – 0(0) – 0(0) –

Hp: Hyperplasia, AD: Adenoma, ADC: Adenocarcinoma. Parenthesis represents the percentage of the tumor incidence.

a : Values represent mean±SD.

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