Morphometric Analysis of Tibial Bone in Three Strains of Mice Using Micro-computed Tomography

Byung-Su Ahn; Joong-Sun Kim; Chang-Geun Lee; Miyoung Yang; Changjong Moon; Jong-Choon Kim; Uhee Jung; Sung-Ho Kim

doi:10.5625/lar.2010.26.3.315

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Ahn, Kim, Lee, Yang, Moon, Kim, Jung, and Kim: Morphometric Analysis of Tibial Bone in Three Strains of Mice Using Micro-computed Tomography

Original Article

Lab. Anim. Res. 2010;26(3):315-318.

Published online: 17 September 2010

DOI: https://doi.org/10.5625/lar.2010.26.3.315

Morphometric Analysis of Tibial Bone in Three Strains of Mice Using Micro-computed Tomography

Byung-Su Ahn¹, Joong-Sun Kim², Chang-Geun Lee², Miyoung Yang¹, Changjong Moon¹, Jong-Choon Kim¹, Uhee Jung³, Sung-Ho Kim^1,^∗

¹College of Veterinary Medicine, Chonnam National University, Gwangju, Korea

²Research Center, Dongnam Institute of Radiological & Medical Sciences (DIRAMS), Busan, Korea

³Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute, Jeongeup, Korea

^∗Corresponding author: Sung-Ho Kim, College of Veterinary Medicine, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Korea Tel: +82-62-530-2837 Fax: +82-62-530-2841 E-mail: shokim@chonnam.ac.kr

Received 14 July 2010 Revised 30 August 2010 Accepted 7 September 2010

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigated the trabecular and cortical bone microarchitecture of tibia in 14-week-old C3H/ HeN, C57BL/6J and ICR mice using micro-computed tomography (micro-CT). Defined volumes of interest were scanned at a resolution of 17 µm (isotropic). The X-ray tube was set at photon energy of 50 kV, current of 200 µA, exposure time 1.2 sec, and a 0.5 mm-thick aluminium filter. For quantification of bone mineral density (BMD), the bone samples were scanned by micro-CT together with 2 calibration phantoms. The image slices were reconstructed using 3-dimensional CT analyzer software. C3H/HeN mice showed significantly higher levels of bone volume fraction, trabecular number and BMD, and lower levels of trabecular separation, structure model index and degree of anisotropy compared to C57BL/6J or ICR mice in trabecular bone area. So the C3H/HeN mouse appeared to be a good model animal for the study on the changes of trabecular bone with high trabecular bone mass.

Keywords: Micro-computed tomography, trabecular bone, microarchitecture, mouse strain

REFERENCES

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Figure 1.

Micro-CT images of the tibia of 14-week-old C3H/HeN mice. Cross sectional view (A), vertical view (B) and reconstructed three-dimensional image (C) are represented.

Table 1.

Microstructural properties of the proximal tibial metaphyseal trabecular bone in three strains of mice

Parameter	C3H/HeN	C57BL/6J	ICR
BV/TV (%)	15.72±4.49^a	6.29±1.39^b	5.50±2.70^b
Tb.Th (µm)	69.92±2.57^a	63.80±7.02^a0	77.32±6.37^c0
Tb.N (1/mm)	02.24±0.57^a	0.98±0.11^b	0.70±0.31^b
Tb.Sp (µm)	275±37^a	391±26^b0	603±150^b
Tb.Pf (1/mm)	09.83±4.97^a	23.79±2.89^b0	19.70±4.73^b0
SMI	01.45±0.24^a	2.13±0.08^b	2.09±0.15^b
DA	02.95±0.37^a	3.51±0.52^a	3.38±0.25^b
BMD (mg/cm³)	318±70^a	14±8^b0	54±11^c

Data are expressed as means±SD (n=6). BV/TV, bone volume/ tissue volume; Tb.Th, trabecular thickness; Tb.N, trabecular number; Tb.Sp, trabecular separation; Tb.Pf, trabecular bone pattern factor; SMI, structure model index; DA, degree of anisotropy; BMD, bone mineral density.

^a,b,c Values with different superscripts in the same parameter are significantly different (P<0.05).

Table 2.

Microstructural properties of the tibial diaphyseal cortical bone in three strains of mice

Parameter	C3H/HeN	C57BL/6J	ICR
BV (mm³)	0.72±0.04^a	0.52±0.02^b	1.01±0.01^c
Po (%)	2.06±1.35^a	0.47±0.44^b	1.54±0.26^a
pMOI (mm⁴)	0.22±0.02^a	0.36±0.03^b	0.74±0.22^c

Data are expressed as means±SD (n=6). BV, bone volume; Po, percent porosity; pMOI, polar moment of inertia.

^a,b,c Values with different superscripts in the same parameter are significantly different (P<0.05).

Table 3.

Microstructural properties of the tibial bone in ovariectomized and sham C3H/HeN mice

Parameter	Sham	Ovariectomy
Trabecular bone
BV/TV (%)	17.54±4.050	3.95±1.49^∗
Tb.Th (µm)	80.55±6.900	64.49±6.50^∗0
Tb.N (1/mm)	2.19±0.51	0.60±0.18^∗
Tb.Sp (µm)	307±560	523±76^∗0
Tb.Pf (1/mm)	8.36±2.91	23.46±4.26^∗0
SMI	1.44±0.20	2.12±0.10^∗
DA	3.06±0.34	4.16±0.93^∗
BMD (mg/cm³)	336±600	97±27^∗
Cortical bone
BV (mm³)	0.77±0.04	0.75±0.08^∗
Po (%)	2.18±1.26	2.84±0.98^∗
pMOI (mm⁴)	0.25±0.02	0.26±0.04^∗

^∗ Significant difference between ovariectomy and sham at P<0.05.

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