Tissue Specific Expression of Lipid Metabolism Related Molecules in Digestive Organs of Miniature Pigs

Ho Jae Han; Seong Soo Kang; Soo Hyun Park

doi:10.5625/lar.2010.26.3.273

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Han, Kang, and Park: Tissue Specific Expression of Lipid Metabolism Related Molecules in Digestive Organs of Miniature Pigs

Original Article

Lab. Anim. Res. 2010;26(3):273-278.

Published online: 17 September 2010

DOI: https://doi.org/10.5625/lar.2010.26.3.273

Tissue Specific Expression of Lipid Metabolism Related Molecules in Digestive Organs of Miniature Pigs

Ho Jae Han, Seong Soo Kang, Soo Hyun Park^∗

Bio-therapy Human Resources Center and Animal Medical Center, College of Veterinary Medicine, Chonnam National University, Gwangju, Korea

^∗Corresponding author: Soo Hyun Park, Bio-therapy Human Resources Center and Animal Medical Center, Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Korea Tel : +82-62-530-2832 Fax: +82-62-530-2809 E-mail: parksh@chonnam.ac.k

Received 11 November 2009 Revised 6 September 2010 Accepted 9 September 2010

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The miniature pig is a very suitable donor species in xenotransplantation of human organs. Lipid metabolism is an important process that involves the creation and degradation of lipids, which is associated with the function of the gastro-intestinal tract. However, the distribution of lipid metabolism related molecules in the gastro-intestinal tract in the miniature pig is unclear. The present study examined the expression of farnesoid X-receptor (FXR), liver X- receptor (LXR), retinoid X-receptor (RXR), liver fatty acid binding protein (L-FABP), fatty acid synthase (FAS) mRNA in the digestive organs of miniature pigs. FXR and LXR mRNA were not expressed in the stomach but were expressed at high and low density in the small and large intestines, respectively. RXR mRNA was expressed in stomach with moderate density, small intestine with high density and in the large intestine with low density. L-FABP and FAS mRNA were expressed in the stomach and large intestine with low density and in the small intestine with high density. L-FABP mRNA was expressed in the liver and kidney with high density, and in pancreas with low density. FAS mRNA was expressed in the liver with high density, and in pancreas and kidney with low density.

Keywords: Miniature pigs, lipid metabolism related molecules, gastro-intestinal tract, mRNA

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Figure 1.

The expression of FXR, LXR, RXR-beta, L-FABP, and FAS mRNA in the fundus, body and antrum of stomach in miniature pigs (A). mRNA expression was evaluated by RT-PCR as described in Materials and Methods nsection. Beta-actin was used as an endogenous control. Lower panel depicts the relative contents of optimal density in stomach (B).

Figure 2.

The expression of FXR, LXR, RXR-beta, L-FABP, and FAS mRNA in the small intestine (duodenum, jejunum and ileum) and large intestine (cecum, colon and rectum) in miniature pigs (A). Lower panel depicts the relative contents of optimal density in small intestine (B) and large intestine (C).

Figure 3.

The expression of FXR, LXR, RXR-beta, L-FABP, and FAS mRNA in the liver, pancreas, and kidney in miniature pigs (A). Lower panel depicts the relative contents of optimal density in liver, kidney, pancreas, and kidney (B).

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