Journal List > Lab Anim Res > v.26(2) > 1053612

Lee, Shin, Yun, Lee, Lee, Kweon, Yun, Suh, Shin, and Seong: Genetic Polymorphism of Jeju Horses by Microsatellite DNA Markers in Korea

Abstract

We determined the genetic diversity and distance between Jeju and Thoroughbred horses by genotyping for 20 microsatellite loci consisting of (TG)n repetitive sequence. The expected heterozygosity ranged from 0.1 to 0.789 in the Jeju horses and from 0.505 to 0.824 in the Thoroughbred horses. Polymorphic information content (PIC) values ranged from 0.09 to 0.709 in the Jeju horses and 0.365 to 0.730 in Thoroughbred horses. There were no significant differences in heterozygosity and PIC values between Jeju and Thoroughbred horsesHowever, LEX035 was estimated relatively high heterozygosity (0.789) and PIC value 0.709) in Jeju horses and LEX050 was respectively 0.824, and 0.730 in the Thoroughbred horses. We may conclude that the genetic differentiation was low between Jeju and Thoroughbred horses. LEX 050, LEX055, LEX059 and LEX 063 could be used as geneticmarkers for differentiating Jeju from Thoroughbred horses.

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Table 1.
Characteristics of microsatellite loci of Jeju and Thoroughbred horses
Primer name Repeat sequence Jeju Horses Thoroughbred horses
Product size(bp) No. of alleles PIC Heterozygosity Product size(bp) No. of alleles PIC Heterozygosity
LEX034 (TG)17 244-252 4 0.627 0.721 244-252 3 0.548 0.653
LEX035 (TG)16 247-261 5 0.709 0.789 247-265 5 0.642 0.732
LEX037 (TG)14 190-194 3 0.586 0.695 188-194 4 0.627 0.721
LEX038 (TG)13 140-146 4 0.632 0.721 142-148 4 0.613 0.712
LEX040 (TG)13 148-154 4 0.653 0.742 148-154 4 0.584 0.684
LEX041 13 (TG)12 148-154 4 0.623 0.721 146-152 4 0.633 0.732
LEX043 (TG)18 238-240 2 0.351 0.479 238-240 2 0.365 0.505
LEX046 18 (TG)18 114-120 4 0.602 0.706 114-120 4 0.665 0.753
LEX047 (TG)14 247-251 3 0.436 0.563 249-253   0.424 0.542
LEX048 (TG)12 169-171 2 0.365 0.505 169-173 3 0.424 0.542
LEX050 (TG)15 118-120 2 0.090 0.100 118-128 5 0.730 0.824
LEX052 (TG)13 197-203 3 0.448 0.582 195-199 3 0.442 0.532
LEX053 (TG)15 127-133 4 0.685 0.774 127-133 4 0.640 0.726
LEX055 (TG)18 199-209 3 0.303 0.353 199-209 3 0.513 0.616
LEX057 (TG)15 162-166 3 0.513 0.616 162-166 3 0.442 0.532
LEX058 (TG)21 228-232 3 0.555 0.658 224-230 3 0.534 0.647
LEX059 (TG)13 224-228 2 0.164 0.189 224-232 3 0.466 0.568
LEX061 (TG)17 130-136 3 0.492 0.611 126-136 5 0.627 0.716
LEX062 (TG)15 190-196 4 0.583 0.680 190-196 4 0.675 0.763
LEX063 15 (TG)16 238-244 2 0.222 0.268 222-244 4 0.645 0.737
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