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Lee, Oh, Park, Lee, Sul, Song, and Kim: Skin Whitening Effects of Sanguisorba officinalis and Stichopus japonicus
Original Article

Lab. Anim. Res. 2010;26(2):127-132.

Published online: 17 June 2010

DOI: https://doi.org/10.5625/lar.2010.26.2.127

Skin Whitening Effects of Sanguisorba officinalis and Stichopus japonicus

Min-Ok Lee1, Hong-Geun Oh2, Sang-Ho Park3, Hyun-A Lee4, Jeong-Dug Sul5, Jeho Song1, ∗, Okjin Kim4, ∗

1Division of Beauty Design & Institute for Better Living, Wonkwang University, Iksan, Korea

2Huvet Co. Ltd, Iksan, Korea

3Korea DNA Valley Co. Ltd, Iksan, Korea

4Center for Animal Resources Development, Wonkwang University, Iksan, Korea

5College of Sport Science, Chung-Ang University, Anseong, Korea

∗Corresponding authors: Jeho Song, Division of Beauty Design & Institute for Better Living, Wonkwang University, 344-2 Shinyoung-dong, Iksan 570-749, Korea Tel: +82-63-850-6895 Fax: +82-63-850-7128 E-mail: sjhao@wku.ac.kr

∗Okjin Kim, Center for Animal Resources Development, Wonkwang University, 344-2 Shinyoung-dong, Iksan 570-749, Korea Tel: +82-63-850-6668 Fax: +82-63-850-7308 E-mail: kimoj@wku.ac.kr

Received 24 February 20109 March 2010       Accepted 27 April 2010

Copyright © 2010 Korean Association for Laboratory Animal Science

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study is aimed to evaluate skin whitening effects of Sanguisorba officinalis and Stichopus japonicus for cosmeceutical ingredients. The extract of Stichopus japonicus showed 61.78% inhibition of tyrosinase activity, and the mixture of Sanguisorba officinalis extract and Stichopus japonicus extract showed 59.14% inhibition of tyrosinase activity. On the clone M-3 cell meloanocyte, the mixture of Sanguisorba officinalis extract and Stichopus japonicus extract showed remarkable inhibition of melanogenesis. Also, those extracts were not irritable in ocular irritation test. It is concluded from these results that Sanguisorba officinalis and Stichopus japonicus have skin whitening effect. It could be used as natural depigmental material in cosmeceutical ingredients.

Keywords: Sanguisorba officinalis, Stichopus japonicus, herb, skin whitening, depigment, cosmeceutical ingredient

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Table 1.
Effects of Sanguisorba officinalis and Stichopus japonicus extracts of on the tyrosinase activity
Concentration (mg/mL) ESL ESS MESS
500 17.48±2.56 32.63±4.11 38.57±0.79
1,000 30.61±4.81 52.31±0.71 56.11±4.62
1,500 49.11±3.67 48.87±3.61 54.27±3.51
2,000 48.24±1.21 61.78±5.89 59.14±2.68

Unit: %. C: Control. ESL: Ethanol extract of Sanguisorba officinalis. ESS: Ethanol extract of Stichopus japonicas. MESS: A mixture of Sanguisorba officinalis extract and Stichopus japonicus extract.

Table 2.
Melanin inhibitory activity of Sanguisorba officinalis and Stichopus japonicus extracts in Clone M-3 cells
Concentration (mg/mL) ESL ESS MESS
C 100 100 100
2.5 34.11±0.46 44.89±1.76 52.90±3.69
5 36.19±3.48 48.23±5.76 55.99±2.41
10 35.41±2.96 49.41±4.21 58.17±3.66
25 44.69±0.96 47.69±3.75 63.80±3.43
50 49.28±1.82 51.34±4.11 68.70±3.87

Unit: %. C: Control. ESL: Ethanol extract of Sanguisorba officinalis. ESS: Ethanol extract of Stichopus japonicas. MESS: A mixture of Sanguisorba officinalis extract and Stichopus japonicus extract.

Table 3.
Eye irritation score of New Zealand White male rabbit treated with Sanguisorba officinalis and Stichopus japonicus extracts
Group Tissue Scores Days Total Score M.O.I.
1 2 3 4 7
  Cornea A×B×5 0 0 0 0 0 0/80  
1 Iris A×5 0 0 0 0 0 0/10 0
  Conjunctiva (A+B+C)×2 (0+0+0)×2 0 0 0 0 0/20  
  Cornea A×B×5 0 0 0 0 0 0/80  
2 Iris A×5 0 0 0 0 0 0/10 0
  Conjunctiva (A+B+C)×2 (0+0+0)×2 0 0 0 0 0/20  
  Cornea A×B×5 0 0 0 0 0 0/80  
3 Iris A×5 0 0 0 0 0 0/10 0
  Conjunctiva (A+B+C)×2 (0+0+0)×2 0 0 0 0 0/20  
  Cornea A×B×5 0 0 0 0 0 0/80  
4 Iris A×5 0 0 0 0 0 0/10 0
  Conjunctiva (A+B+C)×2 (0+0+0)×2 0 0 0 0 0/20  

A: Redness, B: Chemosis, C: Discharge. Group 1: control, 2: treated with Sanguisorba officinalis extract, 3: treated with Stichopus japonicus extract, 4: treated with the mixture of Sanguisorba officinalis extract and Stichopus japonicuss extract.

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