Doublecortin-immunoreactive Neuroblasts in Each Layer of the Main Olfactory Bulb After Transient Cerebral Ischemia in Gerbils

Jung Hoon Choi; Ki-Yeon Yoo; Ok kyu Park; Choong Hyun Lee; In Koo Hwang; Hyung-Cheul Shin; Moo-Ho Won

doi:10.5625/lar.2010.26.1.121

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Choi, Yoo, Park, Lee, Hwang, Shin, and Won: Doublecortin-immunoreactive Neuroblasts in Each Layer of the Main Olfactory Bulb After Transient Cerebral Ischemia in Gerbils

Original Article

Lab. Anim. Res. 2010;26(1):121-125.

Published online: 17 March 2010

DOI: https://doi.org/10.5625/lar.2010.26.1.121

Doublecortin-immunoreactive Neuroblasts in Each Layer of the Main Olfactory Bulb After Transient Cerebral Ischemia in Gerbils

Jung Hoon Choi^1,², Ki-Yeon Yoo^1,², Ok kyu Park¹, Choong Hyun Lee¹, In Koo Hwang³, Hyung-Cheul Shin^2,⁴, Moo-Ho Won^1,^2,^∗

¹Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon, Korea

²Institute of Natural Medicine, Hallym University, Chuncheon, Korea

³Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, Korea

⁴Department of Physiology, College of Medicine, Hallym University, Chuncheon, Korea

^∗Corresponding author: Moo-Ho Won, Department of Anatomy, College of Medicine, Hallym University, 39 Hallymdaehakgil, Chuncheon, Gangweon 200-702, Korea Tel: +82-33-248-2522 Fax: +82-33-256-1614 E-mail: mhwon@hallym.ac.kr

Received 10 November 2009 Revised 23 December 2009 Accepted 1 February 2010

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Neurogenesis in the adult brain occurs continuously throughout life. The main olfactory bulb (MOB) is the first central relay of the olfactory system. We examined proliferation of newly generated cells in each layer of the gerbil MOB after 5 min of transient cerebral ischemia using doublecortin (DCX), a marker of neuronal progenitors. Many DCX immunoreactive neuroblasts were found in the all layers of the MOBs of control and ischemia groups. Ten to 15 days after ischemia/reperfusion, no difference in numbers of DCX immunoreactive neuroblasts was found in the MOB. Thirty days after ischemia/reperfusion, significant increase of DCX immunoreactive cells was observed in all layers of ischemic MOB. This result indicates that neuroblasts increase in the MOB from 30 days after transient cerebral ischemia in gerbils.

Keywords: Doublecortin, main olfactory bulb, migration, transient ischemia

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Figure 1.

Low magnification of DCX immunoreactivity in the gerbil MOB of control (A, C, E and G) and ischemia-operated groups (B, D, F and H) after ischemia/reperfusion (I/R). DCX immunoreactive cells are distributed throughout the MOB. EPL, external plexiform layer; GCL, granule cell layer; GL, glomerular layer; RMS, rostral migratory stream. Bar=200 µm.

Figure 2.

High magnification of DCX immunoreactivity in the glomerular (GL), external plexiform layers (EPL), granule cell layers (GCL) and rostral migratory stream (RMS) in the gerbil MOB of control (A and B) and ischemia-operated groups (C–J). Arrows indicate DCX immunoreactive neuronal precursors. Note the increase in DCX immunoreactive neuroblasts in all layers of MOB 30 days after I/R. Bar=50 µm.

Figure 3.

Changes in DCX immunoreactive neuroblasts in the GL (A), EPL (B) and GCL (C) after transient cerebral ischemia. (n=7 per group; ∗P<0.05, significantly different from the control-group). The bars indicate the means±SEM.

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