Journal List > Allergy Asthma Immunol Res > v.7(6) > 1052541

Wang, Zhang, Zhang, Liang, Ji, and Wang: Is Folate Status a Risk Factor for Asthma or Other Allergic Diseases?

Abstract

Purpose

It is controversial whether folate status is a risk factor for the development of asthma or other allergic diseases. This study was conducted to investigate whether indirect or direct exposure to folate and impaired folate metabolism, reflected as methylene-tetrahydrofolate reductase (MTHFR) C677T polymorphism, would contribute to the development of asthma and other allergic diseases.

Methods

Electronic databases were searched to identify all studies assessing the association between folate status and asthma or other allergic diseases. Two reviewers independently assessed the eligibility of studies and extracted data. The relative risk (RR) or odds ratio (OR) with 95% confidence intervals (CI) was calculated and pooled.

Results

Twenty-six studies (16 cohort, 7 case-control, and 3 cross-sectional studies) were identified. Maternal folic acid supplementation was not associated with the development of asthma, atopic dermatitis (AD), eczema, and sensitization in the offspring, whereas exposure during early pregnancy was related to wheeze occurrence in the offspring (RR=1.06, 95% CI=[1.02-1.09]). The TT genotype of MTHFR C677T polymorphism was at high risk of asthma (OR=1.41, 95% CI=[1.07-1.86]).

Conclusions

It is indicated that maternal folic acid supplementation during early pregnancy may increase the risk of wheeze in early childhood and that the TT genotype of MTHFR C677T polymorphism impairing folic acid metabolism would be at high risk of asthma development. These results might provide additional information for recommendations regarding forced folate consumption or folic acid supplements during pregnancy based on its well-established benefits for the prevention of congenital malformations. However, currently available evidence is of low quality which is needed to further elucidate.

INTRODUCTION

The prevalence of asthma has dramatically increased during the past decades as in other allergic diseases, but underlying mechanisms that account for this remarkable trend remain unclear.12 This allergy epidemic has been reported in relation to changes in environmental factors, which has generated intense interest in changes of lifestyle such as dietary patterns. One particular dietary component that has recently received much attention is folate, which functions as an essential cofactor for one-carbon transfers, primarily methyl groups, and relates to a complex network of biological processes that are vital to growth.3 Thus, it could directly influence the propensity for epigenetic modifications and might tilt the immunophenotypic balance in favor of allergic disease.
Since maternal folic acid (a synthetic form of folate) supplementation before and during pregnancy has been widely recommended to reach a protective effect on the occurrence of neural tube defects45 as well as other congenital malformations6 for many years, it has been questioned whether folic acid supplementation might be associated with the development of adverse health outcomes in the offspring. Previous findings in mice suggested that maternal dietary supplementation with methyl donors (including folic acid) leads to allergic disease in the offspring,7 and similar conditions were observed in humans.89 These results raised concern about possible adverse effects of folic acid supplementation during pregnancy on respiratory and allergic outcomes in the offspring. However, several studies have not detected any relationship.10111213 In addition, studies conducted to detect the role of impaired folate metabolism, reflected by polymorphism of the methylene-tetrahydrofolate reductase (MTHFR) C677T in the development of asthma or other atopic diseases, have reported inconsistent results.1415
Although several reviews do not support an exact effect of maternal folate status on asthma risk, the timing, course, and dose effect of folate status, and the association between MTHFR C667T polymorphisms and asthma risk are still of increasing interest.16171819 There is no adequate evidence to exclude such effects, either.161819 Therefore, we performed this comprehensive systematic review and meta-analysis of existing evidence to answer the following questions: (1) whether indirect or direct exposure to folate is associated with the development of asthma and other allergic diseases, including atopic dermatitis (AD), eczema, and sensitization; (2) whether there is a dose-dependent relationship between folate status and allergic diseases; (3) whether this relationship could be interpreted by exposure timing; and (4) whether the manifestations could be objectively measured.

MATERIALS AND METHODS

Searching and selection

Comprehensive literature search was performed using databases MEDLINE (PubMed), Cochrane Central, Web of Science, and EMBASE until August 2014. The following terms "folic" or "folate" in combination with "asthma," "wheeze," "allergy," "allergic," "AD," "eczema," "rhinitis," or "hay fever" was used as search terms to identify potential studies. The search strategies were adjusted on the basis of the characteristics of each database. Reference lists of all identified potential studies were manually screened to obtain other relevant articles. No language or initial time restriction was performed.
All trials conducted on humans involving the association of folate status and asthma or other allergic diseases were included except only abstracts or no available data. Replicative publications on the same population with the same outcomes (for example, Thuesen 201020 and Husemoen 200614) were evaluated, one of which with the largest sample size was included when performed meta-analysis.

Data extraction and management

Data was independently extracted by 2 reviewers (T.W. and H.P.Z.). For each study, the following details were extracted: study characteristics (country, study design, and sample size), folate status (reported as maternal use, personal use, objective measure, or gene polymorphism), the duration of follow-up, factors adjusted for the analysis, definition, and estimates of outcomes. To avoid confusing, variations in the definitions used for each outcome in original studies were unified. In this study, atopy was defined as any positive skin prick test (SPT), and sensitization was defined as a positive test for specific immunoglobulin E (sIgE) to at least one of the allergens tested. The Newcastle-Ottawa quality assessment scale was used to evaluate the quality of eligible studies.21 A study with score ≥6 was considered to be of sufficient quality for our meta-analysis. In cases of disagreement, consensus was achieved following discussion with a third adjudicator (G.W.).

Statistical analysis

We followed the guidelines recommended by the Meta-analysis Of Observational Studies in Epidemiology (MOOSE) Group.22 For dichotomous outcomes, the relative risk (RR) or odds ratio (OR) with 95% confidence intervals (CI) was calculated. When original data had been adjusted for potential confounding factors, adjusted estimates were pooled using the generic inverse variance method. The associations between maternal folate/folic acid status at different gestation periods (prepregnancy, early pregnancy, including the first trimester, whole pregnancy or other periods) and asthma/wheeze or other allergic outcomes were investigated. Heterogeneity was examined using the chi-square test with P<0.10 indicating significant heterogeneity, and a I-square (I2) statistic greater than 50% indicated moderate or high heterogeneity. The fixed-effects model was used for the meta-analysis if there was no significant heterogeneity (I2<50%); otherwise, the random-effects model. The Z test was used to determine the significance of the pooled estimates. Funnel plots and the Egger test were used to investigate study size effects indicating possible publication bias when outcomes were reported in at least 10 studies. Statistical analysis was conducted with Stata 11.0 (Stata Corp. LP, College Station, TX, USA), and a 2-sided P value less than 0.05 was regarded as significant.
The quality of the evidence related to the estimation of the association between maternal folic acid supplementation and asthma/wheeze or other allergic diseases in childhood or between MTHFR C667T polymorphisms and asthma risk followed the suggestions of the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) Working Group by using the GradePro software (version 3.6).23

RESULTS

Trials included, study characteristics and quality of reporting

The primary search strategy initially yielded a total of 1,814 citations from MEDLINE (PubMed) (n=141), Cochrane Central (n=29), Web of Science (n=297), and EMBASE (n=1,347). After removing duplicates and scanning the titles and abstracts, 23 articles were left over. After full-text reading and reference tracing, 26 eligible studies 89101112131520242526272829303132333435363738394041 remained (Fig. 1).
The characteristics of the studies included are summarized in Tables S1, 2, 3 as supplementary data. Among these studies, 16 were cohort,891011121320242731343536373841 7 were case-control,25262829323339 and 3 were cross-sectional153040 studies. The studies were undertaken in the US, the UK, the Netherlands, Denmark, Finland, Australia, Norway, Egypt, Korea, Japan, and India. The sample size ranged from 40 to 32,077. In the cohort studies, the follow-up duration ranged from 1 to 8 years. In birth cohort studies, the follow-up duration was more than 1 year for wheeze and 3 years for asthma. No eligible studies involving the development of hay fever were found.
Among the cohort studies, 12 (n=60,560) investigated the association between maternal folate exposure and asthma/wheeze incidence,8910111213242731373841 while 4 (n=9,073) investigated the relationship between folate status at baseline and progress of concerned diseases.20343536 Of all the case-control studies, 5 (n=5,023) investigated the association between folate status and asthma/wheeze,2628293339 and the remaining 2 (n=462) evaluated the association between folate status and AD.2532
Of the cross-sectional studies, 3 (n=10,891) investigated the association between folate status and targeted diseases.153040 These studies mainly focused on the association of measurable folate concentration and the genotypes of subjects with specific outcomes.
The quality score of cohort studies ranged from 6 to 9 (mean 7.63, SD 1.09) (Table S1 for supplementary data), and that of case-control studies ranged from 4 to 8 (mean 6.71, SD 1.50) (Table S2 for supplementary data). All studies except 133 described the definition of specific outcomes.

Folate and asthma

The incidences of asthma in the offspring with maternal folic acid supplementation during pregnancy (compared to no use) were pooled in 3 cohort studies.101112 There was an insignificant overall effect of maternal folic acid supplementation on the risk of asthma (RR=1.04, 95% CI=[0.94-1.16]). Also, no statistically significant differences in such relationships were observed before conception (RR=0.98, 95% CI=[0.73-1.33]), during early pregnancy (RR=0.98, 95% CI=[0.78-1.23]), or during other periods (RR=1.03, 95% CI=[0.92-1.16]) (Table 1).
Because of significant heterogeneity in measurement and outcome reporting, data about the relationship of the measurable folate level with asthma risk was not pooled. Two studies investigated the association between the measurable maternal folate level and asthma in the offspring. The cohort study by Magdelijns et al.12 showed that higher maternal intracellular folic acid levels in pregnancy tend to decrease risk of childhood asthma in a dose-dependent manner (P for trend=0.05). The case-control study by Haberg et al.26 found a trend of increasing risk of asthma for children aged 3 years across quintiles of maternal plasma folate levels in pregnancy (P for trend=0.006).
Five studies investigated the relationship of maternal folate concentration with risk of asthma. No significant association between the serum folate level and asthma onset293034 or airway inflammation features of asthma (FENO, total IgE level, or the number of positive SPT)36 was found in 3 studies. Thuesen et al.20 found a significant inverse association between serum folate levels and risk of physician-diagnosed asthma, which is similar to that of a previous report.39 Recently, van der Valk et al.34 showed that folate levels of children at birth did not predict asthma-related outcomes up to the age of 6 years.
Four studies of case-control or cross-sectional design explored the association between MTHFR C677T polymorphism and asthma,15202733 but only 2 provided available effect measures.1520 No significant association was found between the MTHFR C677T CT genotype and asthma development (OR= 0.98, 95% CI=[0.82-1.17]), but the TT genotype was at risk of asthma in adults (OR=1.41, 95% CI=[1.07-1.86]) (Table 2 and Fig. 2).

Folate and wheeze

Two studies explored the relationship between maternal folic acid supplementation during pregnancy (compared to no use) and wheeze incidence in the offspring,1112 and showed the insignificant overall effect of folic acid supplementation on the risk of wheeze (RR=1.05, 95% CI=[0.95-1.15]) (Table 1).
Three cohort studies investigated the relation of maternal folic acid supplementation during early pregnancy with the occurrence of wheeze in the offspring81213 and found a significant pooled effect of maternal folic acid supplementation during early pregnancy on the incidence of offspring wheeze (RR= 1.06, 95% CI=[1.02-1.09]) (Fig. 3). No association was found between maternal folic acid supplementation during other periods and risk of wheeze in childhood (RR=1.01, 95% CI=[0.98-1.03]).
No significant dose-response relationship was observed between maternal folic acid supplementation during pregnancy and the risk of wheeze.102431 Furthermore, a recently published study did not find that early-life folate levels measured at ages 2, 4, 6, and 8 years were associated with asthma or wheeze at 6 years.35 Kiefte-de Jong et al.13 found no association between maternal MTHFR C677T polymorphism and wheeze in the offspring.

Folate and other allergic diseases

No association of maternal folic acid supplementation during early pregnancy with the occurrence of offspring AD was found (RR=1.15, 95% CI=[0.91-1.45]) (Table 1). For eczema and sensitization reflected by sIgE status in the offspring, the association also did not reach statistically significant difference (Table 1).
Magdelijns et al.12 also did not observe the relationship between maternal use of folic acid supplements during pregnancy and increased risk of AD at 2 years. A study by Oh et al.25 found that intake of antioxidants nutrients, including folic acid, was negatively associated with AD development (OR=0.37, 95% CI=[0.18-0.73]). A cohort study by Kim et al.41 found that a maternal serum folate level of ≥9.5 ng/mL during mid-pregnancy was inversely associated with AD risk at 24 months (OR=0.52, 95% CI=[0.31-0.88]). A case-control study by Shaheen et al.32 found that serum folic acid levels were lower in AD patients than in the control subjects, although this did not reach statistical significance. Kiefte-de Jong et al.13 found no association between maternal MTHFR C677T polymorphism and AD in the offspring.
Two studies investigated the association of the MTHFR C677T polymorphism with atopy or sensitization.1520 Pooled analyses did not indicate the association between the MTHFR C677T polymorphism and atopy or sensitization (Table 2).

Assessment of the quality of evidence for the association between folate status and the development of asthma/wheeze or other allergic diseases

Evidence from observational studies was graded from low quality in term of the relatively higher level of heterogeneity or higher risk bias compared to randomized controlled trials.42 Furthermore, we performed the analyses at consistent exposure time that would reduce unexplained heterogeneity of the results. As a result, the quality of evidence related to the estimation of association between maternal folic acid supplementation and asthma/wheeze in childhood was very low (Table 3). Likewise, although genotyping is an objective definition, the quality of evidence related to the estimation of the association between MTHFR C667T polymorphisms and asthma, atopy, or sensitization risk was graded as low (Table 4). The overall quality of evidence was not upgraded due to lack of large effects or dose-response relationships.

DISCUSSION

Along with an increasing number of studies investigating the association between folate status and allergic outcomes, conflicts in this field have been on the rise. Thus, current metaanalyses provide no evidence for the relationship of maternal folic acid supplementation during pregnancy with asthma, AD, eczema, or sensitization in the offspring except that an increased risk of wheeze in childhood related to maternal folic acid supplementation during early pregnancy was observed. In addition, our meta-analysis found no evidence for any association between MTHFR C677T polymorphism and targeted outcomes except that the TT genotype was associated with asthma.
Blatter et al.16 reviewed the relationship between folate and asthma and did not support excessive worry about the adverse effect of maternal folate supplementation during pregnancy on asthma, although there was no adequate data to exclude a weak or modest effect. Brown et al.19 summarized epidemiologic evidence from 10 large prospective cohort studies supporting or refuting the relationship between maternal folate exposure in pregnancy and asthma/allergy in the offspring. A previous meta-analysis conducted by Crider et al.18 did not support the association between folic acid supplementation in the periconceptional period or the first trimester and risk of asthma, but the sensitivity analysis of combined asthma and wheeze showed a significant association. Given a diagnosis of asthma is difficult to establish in infants and toddlers but a diagnosis of symptoms, such as wheeze, are more suitable, it seems better to evaluate the effect of maternal folic acid supplementation during pregnancy on wheeze rather than asthma. A study by Haberg et al.8 reviewed in the 2 meta-analyses reported an increased risk of wheeze between the ages of 6 and 18 months, which is consistent with the result of Bekkers' study11 in which an increased risk of wheeze was reported at age 1 year but not at later ages. Bekkers et al.11 evaluated the effects of maternal folic acid supplementation during pregnancy on wheeze or asthma, respectively, from age 1 or 3 years to 8 years and reported an increased risk association with wheeze but not asthma. Thus, we speculated that not all children with wheeze in early life has a diagnosis of asthma in later life and as time goes on, the effect of maternal folate status on respiratory health in the offspring goes down. However, both of these findings were driven by the large cohort study conducted by Haberg et al.,8 which weighted 89% of the summary estimate in the Crider's study18 and 92% in this study. To address the question of a possible causal association between maternal folic acid status in pregnancy and risk of asthma or wheeze in children, a number of additional studies or analyses should be undertaken considering the misclassification of wheeze and asthma with age factor and follow-up duration.
To better understand the possible association, timing of folate intake during pregnancy should be taken into consideration. Since most clinical trials reviewed in this article provided direct evidence that there is no association between maternal folic acid supplementation during pregnancy and asthma,101112 wheeze,111213 shortness of breath,13 AD,1213 or eczema,1112 objective measurements, including allergic sensitization and bronchial hyperresponsiveness, should be made.11 Nevertheless, studies referred to maternal folic acid supplementation at different periods during pregnancy seemed to have different results. Whitrow et al.9 reported that maternal folic acid supplementation in late pregnancy (30-34 weeks) increased the risk of childhood asthma at 3.5 years. Bekkers et al.11 assessed the risk at similar exposure time (median 33 weeks) and reported an increased risk of wheeze at age 1 year. Moreover, Haberg et al.8 found that early pregnancy (any 4-week-period during weeks 0-12 in pregnancy) increased the risk of childhood wheeze in infants up to 18 months. Because widespread DNA methylation programming occurs in the early embryo, the association between maternal folate status during early pregnancy and respiratory health in early childhood might receive more attention.
Although several studies measured direct or indirect folate exposure and investigated a dose-respond association of folate with allergic outcomes, it is not possible to do quantitative analyses due to diverse exposure assessments. Both positive132631 and negative122030 correlations were observed in current evidence. It is possible that these inconsistences reflect complex relationships between direct or indirect folate exposure and the risk of asthma/wheeze or other allergic outcomes. Recent studies showed that folate levels measured in early life did not affect asthma-related outcomes.3435 Interestingly, another study conducted by Lin et al.36 found "bell-shaped" relationships between serum folate and some inflammatory biomarkers of asthma. Likewise, nonlinear relationships were found between serum folate levels and fractional exhaled nitric oxide, total IgE, or the frequency of positive SPTs. The folate level in the second quartile was significantly associated with increased total IgE when compared to the first quartile (the lowest). However, the levels of inflammatory biomarkers in the third or fourth (the highest) quartiles were similar to those in the first quartile and had no association with folate levels. Therefore, high-quality evidence is needed to interpret this complex network. For future studies, objective measurements can be included, and plasma folate concentration might be a better biomarker. In addition, measurements should be taken into account to evaluate not only indirect folate exposure resulting from mothers during pregnancy, but also the folate status of subjects themselves. The dose, timing, and source of folate exposure should also be considered.
The MTHFR gene encodes the protein for making an enzyme called methylene tetrahydrofolate reductase that catalyzes the conversion of 5, 10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, which is required for the conversion of homocysteine to methionine. MTHFR C677T polymorphism was assumed to be associated with the risk of asthma. An association was reported by Zhou et al.33 that the TT genotype significantly increases the risk of atopic asthma compared to all of the CC and CT genotypes. However, subsequent studies found no association between maternal MTHFR C677T polymorphism and asthma,27 wheeze,13 or AD13 in the offspring or mothers themselves.27 Our meta-analysis found no evidence of the association between MTHFR C677T polymorphism and targeted outcomes except that the TT genotype was associated with asthma. In addition, a previous study found a significant interaction between MTHFR C677T and folate levels.34 Because DNA genotyping is assigned at birth and as an objective measurement, utilizing such methods could help reduce measurement biases and help explore a causal association. Thus, intrinsic genetic factors that could impair folate metabolism for a long time might play an important role in the occurrence and development of asthma. However, because folate status is largely influenced by dietary intake, this hypothesis still needs to be confirmed by further studies, and interpretations of these complex relationships should be presented with caution.
Although this study has referred to almost all of the existing evidence we could acquire, limitations of this study should be noted. First, since the articles included in this study were not randomized controlled trials, some obvious or hidden confounding factors may be unavoidable more or less. Second, potential risk of bias resulting from differences in study design, outcomes assessment, measurements or follow-up performance might produce heterogeneity. Third, the dose-response relationship was not analyzed due to limited eligible studies. Fourth, although asthma or other allergic diseases are associated with both genetic and environmental effects,43 it is difficult to analyze potential interactions between MTHFR C677T polymorphism and environmental factors, such as dietary intake, due to lack of data.
In conclusion, our study indicates that maternal folic acid supplementation during early pregnancy might increase the risk of wheeze in early childhood and that the TT genotype of MTHFR C677T polymorphism impairing folic acid metabolism would be at high risk of asthma development. These results can provide additional information for recommendations regarding forced folate consumption or folic acid supplementation in pregnancy based on its well-established benefits for the prevention of congenital malformations. Further studies are needed to elucidate whether there is a critical window of susceptibility that may reduce risk of neural tube defects without increased risk of asthma or other allergic diseases.

Figures and Tables

Fig. 1

Flowchart of study selection.

aair-7-538-g001
Fig. 2

The relationship between MTHFR C677T polymorphism and asthma risk. (A) CT vs CC; (B) TT vs CC. OR, odds ratio; CI, confidence intervals.

aair-7-538-g002
Fig. 3

The relationship between maternal folic acid supplementation during early pregnancy and risk of wheeze in childhood. *Four weeks before conception until 8 weeks after conception and/or during the first trimester of pregnancy. RR, risk ratio; CI, confidence intervals.

aair-7-538-g003
Table 1

Effects of folate status on the development of asthma, wheeze, or other allergic diseases

aair-7-538-i001
Outcome Exposure timing types of folic acid Timing of assessment Folic acid use, RR (95% CI)* Heterogeneity (I2, %)
Asthma Any use101112 At 6 yr10, 3-8 yr11, at 6-7 yr12 1.04 (0.94-1.16) 0.0
Pre-pregnancy910 At 3.5 yr and 5.5 yr9, at 6 yr10 0.98 (0.73-1.33) 0.0
Early pregnancy91012 At 3.5 yr and 5.5 yr9, at 6 yr10, at 6-7 yr12 0.98 (0.78-1.23) 0.0
Other period in pregnancy1112 3-8 yr11, at 6-7 yr12 1.03 (0.92-1.16) 0.0
Wheeze Any use1112 1-8 yr11, until 6-7 yr12 1.05 (0.95-1.15) 0.0
Early pregnancy81213 6-18 mo8, until 6-7 yr12, up to 4 yr13 1.06 (1.02-1.09) 0.0
Other period in pregnancy81112 6-18 mo8, 1-8 yr11, until 6-7 yr12 1.01 (0.98-1.03) 0.0
Atopic Dermatitis Early pregnancy1213 at 2 yr12, up to 4 yr13 1.15 (0.91-1.45) 0.0
Eczema Other period in pregnancy1112 1-8 yr11, until 6-7 yr12 0.99 (0.89-1.10) 0.0
Sensitization Other period in pregnancy1112 At 8 yr11, at 2 yr12 0.88 (0.75-1.03) 0.0

*vs no folic acid use; P=0.001. RR, risk ratio; CI, confidence intervals.

Table 2

The relationship between MTHFR C677T polymorphism and the risk of asthma or other allergic diseases

aair-7-538-i002
Outcome Genotypes OR (95% CI)* Heterogeneity (I2, %)
Asthma CT genotype1520 0.98 (0.82-1.17) 0.0
TT genotype1520 1.41 (1.07-1.86) 21.5
Atopy CT genotype1527 1.05 (0.92-1.19) 0.0
TT genotype1527 0.93 (0.76-1.14) 0.0
Sensitization CT genotype1520 0.99 (0.89-1.10) 0.0
TT genotype1520 1.03 (0.86-1.23) 0.0

*vs the CC genotype; P=0.014. OR, odds ratio; CI, confidence intervals.

Table 3

The quality of evidence assessment of association between maternal folic acid supplementation and asthma/wheeze in childhood by the GRADE approach

aair-7-538-i003
Outcomes Exposure timing Relative effect (95% CI) No. of participants (studies) Quality of evidence (GRADE)
Asthma in childhood
Pregnancy101112 RR=1.04 (0.94-1.16) 7,925 (n=3) ⊕⊕⊖⊖Low 1
Pre-pregnancy910 RR=0.98 (0.73-1.33) 2,056 (n=2) ⊕⊖⊖⊖Very Low 2
Early pregnancy91012 RR=0.98 (0.78-1.23) 4,696 (n=3) ⊕⊕⊖⊖Low 3
Other period in pregnancy1112 RR=1.03 (0.92-1.16) 6,426 (n=2) ⊕⊕⊖⊖Low 4
Wheeze in childhood
Pregnancy1112 RR=1.05 (0.95-1.15) 6,426 (n=2) ⊕⊕⊖⊖Low 5
Early pregnancy81213 RR=1.06 (1.02-1.09) 43,459 (n=3) ⊕⊕⊖⊖Low 6
Other period in pregnancy81112 RR=1.01 (0.98-1.03) 38,503 (n=3) ⊕⊕⊖⊖Low 7
Atopic Dermatitis Early pregnancy1213 RR=1.15 (0.91-1.45) 11,382 (n=2) ⊕⊖⊖⊖Very Low 8
Eczema Other period in pregnancy1112 RR=0.99 (0.89-1.10) 6,426 (n=2) ⊕⊕⊖⊖Low 9
Sensitization Other period in pregnancy1112 RR=0.88 (0.75-1.03) 6,426 (n=2) ⊕⊕⊖⊖Low 10

1 No factors exist that can reduce or increase the quality of evidence.

2 (-1 imprecision) 95% CI overlaps no effect (CI includes RR of 1.0) and CI fails to exclude 0.75 and 1.25 though the sample size is very large.

3 No factors exist that can reduce or increase the quality of evidence.

4 No factors exist that can reduce or increase the quality of evidence.

5 No factors exist that can reduce or increase the quality of evidence.

6 No factors exist that can reduce or increase the quality of evidence.

7 No factors exist that can reduce or increase the quality of evidence.

8 (-1 imprecision) 95% CI overlaps no effect (CI includes RR of 1.0) and CI fails to exclude 1.25 though the sample size is very large.

9 No factors exist that can reduce or increase the quality of evidence.

10 No factors exist that can reduce or increase the quality of evidence.

RR, risk ratio; CI, confidence intervals.

Table 4

The quality of evidence assessment of association MTHFR C667T polymorphisms and asthma risk by the GRADE Approach

aair-7-538-i004
Outcomes Comparisons Relative effect (95% CI) No. of participants (studies) Quality of evidence (GRADE)
Asthma CT vs CC1520 OR=0.98 (0.82-1.17) 7,991 (n=2) ⊕⊕⊖⊖Low 1
TT vs CC1520 OR=1.41 (1.07-1.86) 7,991 (n=2) ⊕⊕⊖⊖Low 2
Atopy CT vs CC1527 OR=1.05 (0.92-1.19) 6,571 (n=2) ⊕⊕⊖⊖Low 3
TT vs CC1527 OR=0.93 (0.76-1.14) 6,571 (n=2) ⊕⊕⊖⊖Low 4
Sensitization CT vs CC1520 OR=0.99 (0.89-1.10) 7,991 (n=2) ⊕⊕⊖⊖Low 5
TT vs CC1520 OR=1.03 (0.86-1.23) 7,991 (n=2) ⊕⊕⊖⊖Low 6

Quality of evidence (GRADE) as in Table 3.

OR, odds ratio; CI, confidence intervals.

ACKNOWLEDGMENTS

This study was partly supported by a grant from National Natural Science Foundation of China (30971326, 31450007, 81171320, 81241002, and 81370122), Sichuan Youth Science and Technology Foundation (No. 2010JQ0008), Youth Science Funding of Sichuan University (2011SCU04B17), and Program for New Century Excellent Talents in University (NCET-12-0380). The funders played no role in study design, data collection and analysis, preparation of the manuscript, or decision to submit the manuscript for publication.

Notes

There are no financial or other issues that might lead to conflict of interest.

References

1. Braman SS. The global burden of asthma. Chest. 2006; 130:4S–12S.
2. Wong GW, Leung TF, Ko FW. Changing prevalence of allergic diseases in the Asia-pacific region. Allergy Asthma Immunol Res. 2013; 5:251–257.
3. Depeint F, Bruce WR, Shangari N, Mehta R, O'Brien PJ. Mitochondrial function and toxicity: role of B vitamins on the one-carbon transfer pathways. Chem Biol Interact. 2006; 163:113–132.
4. MRC Vitamin Study Research Group. Prevention of neural tube defects: results of the Medical Research Council Vitamin Study. Lancet. 1991; 338:131–137.
5. Czeizel AE, Dudás I, Paput L, Bánhidy F. Prevention of neural-tube defects with periconceptional folic acid, methylfolate, or multivitamins? Ann Nutr Metab. 2011; 58:263–271.
6. De-Regil LM, Fernández-Gaxiola AC, Dowswell T, Peña-Rosas JP. Effects and safety of periconceptional folate supplementation for preventing birth defects. Cochrane Database Syst Rev. 2010; CD007950.
7. Hollingsworth JW, Maruoka S, Boon K, Garantziotis S, Li Z, Tomfohr J, et al. In utero supplementation with methyl donors enhances allergic airway disease in mice. J Clin Invest. 2008; 118:3462–3469.
8. Håberg SE, London SJ, Stigum H, Nafstad P, Nystad W. Folic acid supplements in pregnancy and early childhood respiratory health. Arch Dis Child. 2009; 94:180–184.
9. Whitrow MJ, Moore VM, Rumbold AR, Davies MJ. Effect of supplemental folic acid in pregnancy on childhood asthma: a prospective birth cohort study. Am J Epidemiol. 2009; 170:1486–1493.
10. Martinussen MP, Risnes KR, Jacobsen GW, Bracken MB. Folic acid supplementation in early pregnancy and asthma in children aged 6 years. Am J Obstet Gynecol. 2012; 206:72.e1–72.e7.
11. Bekkers MB, Elstgeest LE, Scholtens S, Haveman-Nies A, de Jongste JC, Kerkhof M, et al. Maternal use of folic acid supplements during pregnancy, and childhood respiratory health and atopy. Eur Respir J. 2012; 39:1468–1474.
12. Magdelijns FJ, Mommers M, Penders J, Smits L, Thijs C. Folic acid use in pregnancy and the development of atopy, asthma, and lung function in childhood. Pediatrics. 2011; 128:e135–e144.
13. Kiefte-de Jong JC, Timmermans S, Jaddoe VW, Hofman A, Tiemeier H, Steegers EA, et al. High circulating folate and vitamin B-12 concentrations in women during pregnancy are associated with increased prevalence of atopic dermatitis in their offspring. J Nutr. 2012; 142:731–738.
14. Husemoen LL, Toft U, Fenger M, Jørgensen T, Johansen N, Linneberg A. The association between atopy and factors influencing folate metabolism: is low folate status causally related to the development of atopy? Int J Epidemiol. 2006; 35:954–961.
15. Thuesen BH, Husemoen LL, Fenger M, Linneberg A. Lack of association between the MTHFR (C677T) polymorphism and atopic disease. Clin Respir J. 2009; 3:102–108.
16. Blatter J, Han YY, Forno E, Brehm J, Bodnar L, Celedón JC. Folate and asthma. Am J Respir Crit Care Med. 2013; 188:12–17.
17. Sharland E, Montgomery B, Granell R. Folic acid in pregnancy - is there a link with childhood asthma or wheeze? Aust Fam Physician. 2011; 40:421–424.
18. Crider KS, Cordero AM, Qi YP, Mulinare J, Dowling NF, Berry RJ. Prenatal folic acid and risk of asthma in children: a systematic review and meta-analysis. Am J Clin Nutr. 2013; 98:1272–1281.
19. Brown SB, Reeves KW, Bertone-Johnson ER. Maternal folate exposure in pregnancy and childhood asthma and allergy: a systematic review. Nutr Rev. 2014; 72:55–64.
20. Thuesen BH, Husemoen LL, Ovesen L, Jørgensen T, Fenger M, Gilderson G, et al. Atopy, asthma, and lung function in relation to folate and vitamin B(12) in adults. Allergy. 2010; 65:1446–1454.
21. Wells GA, Shea B, O'Connell D, Peterson J, Welch V, Losos M, et al. The Newcastle-Ottawa Scale (NOS) for assessing the quality of nonrandomised studies in meta-analyses. Ottawa: Ottawa Hospital Research Institute;2000.
22. Stroup DF, Berlin JA, Morton SC, Olkin I, Williamson GD, Rennie D, et al. Meta-analysis of observational studies in epidemiology: a proposal for reporting. Meta-analysis Of Observational Studies in Epidemiology (MOOSE) group. JAMA. 2000; 283:2008–2012.
23. Brozek J, Oxman A, Schünemann H. The GRADE Working Group. GRADEpro. Version 3.6 for Windows [Computer Program]. Hamilton: McMaster University;2011.
24. Miyake Y, Sasaki S, Tanaka K, Hirota Y. Maternal B vitamin intake during pregnancy and wheeze and eczema in Japanese infants aged 16-24 months: the Osaka Maternal and Child Health Study. Pediatr Allergy Immunol. 2011; 22:69–74.
25. Oh SY, Chung J, Kim MK, Kwon SO, Cho BH. Antioxidant nutrient intakes and corresponding biomarkers associated with the risk of atopic dermatitis in young children. Eur J Clin Nutr. 2010; 64:245–252.
26. Håberg SE, London SJ, Nafstad P, Nilsen RM, Ueland PM, Vollset SE, et al. Maternal folate levels in pregnancy and asthma in children at age 3 years. J Allergy Clin Immunol. 2011; 127:262–264.
27. Granell R, Heron J, Lewis S, Davey Smith G, Sterne JA, Henderson J. The association between mother and child MTHFR C677T polymorphisms, dietary folate intake and childhood atopy in a population-based, longitudinal birth cohort. Clin Exp Allergy. 2008; 38:320–328.
28. Bueso AK, Berntsen S, Mowinckel P, Andersen LF, Lødrup Carlsen KC, Carlsen KH. Dietary intake in adolescents with asthma--potential for improvement. Pediatr Allergy Immunol. 2011; 22:19–24.
29. Farres MN, Shahin RY, Melek NA, El-Kabarity RH, Arafa NA. Study of folate status among Egyptian asthmatics. Intern Med. 2011; 50:205–211.
30. Matsui EC, Matsui W. Higher serum folate levels are associated with a lower risk of atopy and wheeze. J Allergy Clin Immunol. 2009; 123:1253–1259.e2.
31. Dunstan JA, West C, McCarthy S, Metcalfe J, Meldrum S, Oddy WH, et al. The relationship between maternal folate status in pregnancy, cord blood folate levels, and allergic outcomes in early childhood. Allergy. 2012; 67:50–57.
32. Shaheen MA, Attia EA, Louka ML, Bareedy N. Study of the role of serum folic acid in atopic dermatitis: a correlation with serum IgE and disease severity. Indian J Dermatol. 2011; 56:673–677.
33. Zou CC, Tang LF, Jiang MZ, Zhao ZY, Hirokazu T, Mitsufumi M. Methylenetetrahydrofolate reductase [correction of reducatase] polymorphism and asthma. Zhonghua Jie He He Hu Xi Za Zhi. 2003; 26:161–164.
34. van der Valk RJ, Kiefte-de Jong JC, Sonnenschein-van der Voort AM, Duijts L, Hafkamp-de Groen E, Moll HA, et al. Neonatal folate, homocysteine, vitamin B12 levels and methylenetetrahydrofolate reductase variants in childhood asthma and eczema. Allergy. 2013; 68:788–795.
35. Okupa AY, Lemanske RF Jr, Jackson DJ, Evans MD, Wood RA, Matsui EC. Early-life folate levels are associated with incident allergic sensitization. J Allergy Clin Immunol. 2013; 131:226–228.e1.
36. Lin JH, Matsui W, Aloe C, Peng RD, Diette GB, Breysse PN, et al. Relationships between folate and inflammatory features of asthma. J Allergy Clin Immunol. 2013; 131:918–920.
37. Nwaru BI, Erkkola M, Ahonen S, Kaila M, Kronberg-Kippilä C, Ilonen J, et al. Intake of antioxidants during pregnancy and the risk of allergies and asthma in the offspring. Eur J Clin Nutr. 2011; 65:937–943.
38. Litonjua AA, Rifas-Shiman SL, Ly NP, Tantisira KG, Rich-Edwards JW, Camargo CA Jr, et al. Maternal antioxidant intake in pregnancy and wheezing illnesses in children at 2 y of age. Am J Clin Nutr. 2006; 84:903–911.
39. Patel BD, Welch AA, Bingham SA, Luben RN, Day NE, Khaw KT, et al. Dietary antioxidants and asthma in adults. Thorax. 2006; 61:388–393.
40. Woods RK, Walters EH, Raven JM, Wolfe R, Ireland PD, Thien FC, et al. Food and nutrient intakes and asthma risk in young adults. Am J Clin Nutr. 2003; 78:414–421.
41. Kim JH, Jeong KS, Ha EH, Park H, Ha M, Hong YC, et al. Relationship between prenatal and postnatal exposures to folate and risks of allergic and respiratory diseases in early childhood. Pediatr Pulmonol. 2015; 50:155–163.
42. Balshem H, Helfand M, Schünemann HJ, Oxman AD, Kunz R, Brozek J, et al. GRADE guidelines: 3. Rating the quality of evidence. J Clin Epidemiol. 2011; 64:401–406.
43. Zhang G, Khoo SK, Mäkelä MJ, Candelaria P, Hayden CM, von Hertzen L, et al. Maternal genetic variants of IL4/IL13 pathway genes on IgE with "Western or Eastern Environments/Lifestyles". Allergy Asthma Immunol Res. 2014; 6:350–356.

Supplementary Materials

Table S1

The characteristics of studies included in the systematic review on the relationship between folate status and asthma, wheeze or other allergic diseases (cohort study)

Table S2

The characteristics of included study (case-control study)

Table S3

The characteristics of included study (cross-section study)

REFERENCES

TOOLS
Similar articles