Abstract
Figures and Tables
![]() | Fig. 2
Effect of fucoxanthin on mRNA expression of transcription factor and enzymes related to lipid metabolism in the liver of rats. Total RNA was isolated using TRI-reagenet and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. SREBP-1C, Sterol regulatory element binding protein; G6PDH, Glucose-6-phosphate dehydrogenase; FAS, Fatty acid synthase; ACC, Acetyl-CoA carboxylase. |
![]() | Fig. 3
Effect of fucoxanthin on mRNA expression of CPT1 in the liver of rats. Total RNA was isolated using TRI-reagenet and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. CPT1, Carnitine palmitoyltransferase-1. |
![]() | Fig. 4
Effect of fucoxanthin on mRNA expression of enzymes related to cholesterol synthesis in the liver of rats. Total RNA was isolated using TRI-reagenet and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. HMG-CoA, Hydroxy-3-methylglutaryl coenzyme A; ACAT, Acyl-CoA cholesterol acyltransferase; LCAT, lecithin-cholesterol acyltransferase. |
![]() | Fig. 5
Effect of fucoxanthin on the mRNA expression of CYP7A1 in the liver of rats. Total RNA was isolated using TRI-reagenet and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. CYP7A1, Cholesterol 7α-hydroxylase1. |
Table 1
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1)NF; normal fat diet, HF; high fat diet, HF + Fxn; high fat diet + 0.2% fucoxanthin (*Fucoxanthin powder contained 5% fucoxanthin extracted from seaweeds)
2)Mineral mixture (per kg): Calcium carbonate anhydrous, 357 g; Potassium phosphate monobasic, 196 g; Potassium citrate tripotassium monohydrate, 70.78 g; Potassium sulfate Sodium chloride, 74 g: Magnesium oxide, 24 g; Ferric citrate, 6.06 g; Zinc carbonate, 1.65 g; Sodium meta-silicate, 1.45 g; Manganous carbonate, 0.63 g; Cupric carbonate, 0.30 g; Chromium potassium sulfate, 0.275 g; Boric acid, 81.5 mg; Sodium fluoride, 63.5 mg; Nickel carbonate, 31.8 mg; Lithium chloride, 17.4 mg; Sodium selenate anhydrous, 10.25 mg; Potassium iodate, 10.0 mg; Ammonium paramolybdate, 6.66 mg; Powdered sucrose, 221.026 g
3)Vitamin mixture (per kg): Nicotinic acid, 3.0 g; Ca Pantothenate, 1.6 g; Pyridoxine HCl, 0.7 g; Thiamin HCl, 0.6 g; Riboflavin, 0.6 g; Folic acid, 0.2 g; Biotin, 0.02 g; Vitamin B12, 2.5 g; Vitamin E, 15.0 g;Vitamin A, 0.8 g;Vitamin D3, 0.25 g;Vitamin K-1, 0.075 g; Powdered sucrose, 974.655 g
References
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