Mutational Analysis of Pro-apoptotic BAD Gene in Non- small Cell Lung Cancer

Jonq Woo Lee; Youn Hwa Soung; Suk Woo Nam; Junq Younq Lee; Nam Jin Yoo; Sug Hyung Lee

doi:10.6058/jlc.2006.5.1.35

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Lee, Soung, Nam, Lee, Yoo, and Lee: Mutational Analysis of Pro-apoptotic BAD Gene in Non- small Cell Lung Cancer

Original Article

J Lung Cancer 2006;5(1):35-38.

Published online: 18 December 2006

DOI: https://doi.org/10.6058/jlc.2006.5.1.35

Mutational Analysis of Pro-apoptotic BAD Gene in Non- small Cell Lung Cancer

Jonq Woo Lee, M.S., Youn Hwa Soung, M.S., Suk Woo Nam, Ph.D., Junq Younq Lee, M.D., Nam Jin Yoo, M.D., Sug Hyung Lee, M.D.

¹Deoartment of Patholoqv, College of Medicine. The Catholic University of Korea, Seoul, Korea

This work was suooorted bv the funds from KOSEF (R01-2004-000-10463)).

Address for correspondence Sug Hvuna Lee, MD. Department of Pathdogy, Colleqe of Me· dicine. The Catholic University of Korea, 505 Banoo-dong, Sochogu, Seoul 137- 701, Korea Tel: 82-2-590-1188 Fax: 82-2-537-6586 E-mail: suhulee@catholio.aakr

Received 4 February 2006 Accepted 3 March 2006

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose:

Evidence exists that deregulation of apoptosis is involved in the mechanisms of cancer development, and the somatic mutations of apoptosis- related genes have been reported in human cancers. Bel- Xᄂ/Bcl-2-assᄋdated death promoter (BAD), a pro-apoptotic member of Bcl-2 family, plays an important role in the intrinsic apoptosis pathway.

Materials and Methods:

To explore the possibility that the genetic alterations of BAD might be involved in the development of human cancers, we analyzed the entire coding region and all splice sites of human BAD gene in 100 human non-small cell lung cancers (NSCLC) by polymerase chain reaction (PCR)-based single-strand conformation polymorphism (SSCP).

Results:

The PCR-SSCP analysis detected no mutation in the entire coding regions and all splice sites of human BAD gene in the 100 NSCLCs.

Conclusion:

The data presented here suggested that BAD gene mutation may not contribute to the pathogenesis of human NSCLCs. (J Lung Cancer 2006;5(1):35-38)

Keywords: Non-small cell lurig cancer, BAD, Apoptosis, Mutation

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Fig. 1.

Representative SSCP of BAD gene in the non-small cell lung cancers. The exon 2 of the BAD gene were amplified by PGR using a specific primer set. The PCR products from the representative cases of non-small cell lung cancers were visualized on SSCP. SSCP of DNA from the non-small cell lung cancers (T) shows no aberrant bands as compared to SSCPs from the normal tissues (N),

Table 1.

Sequence of SSCP Primer Sets for BAD

Primers	Exon	Sequence	Annealing Tm (^°C)(bp)	Size (bp)
1A	1	F 5’-GCCCCAGCTGTGCCTTGACTACG-3’	58	172
		R 5’-GGCCTGGCGATGTGCTTGC-3’
1A	1	F 5’-CAGCTCTGCAGAGAGGGGCCTGG-3’	66	195
		R 5’-CAGTGGGCTGGGGGTGAGGTGTC-3’
2A	2	F 5’-GAGGAGCTGAGATTAACGTCGCC-3’	56	187
		R 5’-CTGTGCTGCCCAGAGGTTGG-3·
2B	2	F 5’-GGAGGACGACGAAGGGATGG-3’	56	187
		R 5’-CGGCCTGGTACTTCAGGTCC-3’
3A	3	F 5’-AGCTGCCATGGTAATCTAACTCA-3’	58	191
		R 5’-GATGTGGAGCGAAGGTCACT-3’
3B	3	F 5’-CTCCAGCTGGACGCGAGTCT-3’	62	175
		R 5’-CTCTTTTTGCATAGGCC-3’

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