Journal List > J Lung Cancer > v.5(2) > 1050654

J Lung Cancer. 2006 Dec;5(2):111-113. Korean.
Published online December 31, 2006.  https://doi.org/10.6058/jlc.2006.5.2.111
©2006 Korean Association for the Study of Lung Cancer
Pro-apoptotic Cytochrome c Gene Mutation is Rare in Non-small Cell Lung Cancers
Young Hwa Soung, Ph.D. and Sug Hyung Lee, M.D.
Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Address for correspondence: Sug Hyung Lee, M.D. Department of Pathology, College of Medicine, The Catholic University of Korea, 505, Banpo-dong, Seocho-gu, Seoul 137-701, Korea. Tel: 82-2-590-1188, Fax: 82-2-537-6586, Email: suhulee@catholic.ac.kr
Received December 03, 2006; Accepted December 11, 2006.

Abstract

Purpose

Several lines of evidence have indicated that the deregulation of apoptosis is involved in the mechanisms of cancer development, and somatic mutations of the apoptosis-related genes have been reported in human cancers. In addition to its role in oxidative phosphorylation, release of cytochrome c from the mitochondrial intermembrane space results in nuclear apoptosis. The aim of this study was to explore whether alteration of cytochrome c gene mutation is a characteristic of human non-small cell lung cancers (NSCLC).

Materials and Methods

In the current study, to detect the somatic mutations in the DNA sequences encoding cytochrome c in 48 NSCLCs, we used polymerase chain reaction (PCR), single-strand conformation polymorphism (SSCP), and DNA sequencing.

Results

The SSCP analysis revealed no mutation in the entire coding regions and all splice sites of human cytochrome c gene in the 48 NSCLCs.

Conclusion

The data presented here indicate that the pro-apoptotic cytochrome c may not be somatically mutated in human NSCLCs, and suggest that NSCLCs may not utilize mutational events of cytochrome c gene in the mechanisms for evading apoptosis.

Keywords: Non-small cell lung cancer; Cytochrome c; Apoptosis; Mutation

Figures


Fig. 1
Representative SSCP of cytochrome c gene in the non-small cell lung cancers. The exon 2 of the cytochrome c gene was amplified by PCR using a specific primer set. The PCR products from the representative cases of non-small cell lung cancers were visualized on SSCP. SSCP of DNA from the non-small cell lung cancers (T) shows no aberrant bands as compared to SSCPs from the normal tissues (N).
Click for larger image

Tables


Table 1
Sequences of PCR Primer Sets for Cytochrome c Gene Amplification
Click for larger image

Notes

This work was supported by the funds from KOSEF (R01-2004-000-10463-0).

References
1. Reed JC. Mechanisms of apoptosis. Am J Pathol 2000;39:1415–1430.
2. Nagata S. Apoptosis by death factor. Cell 1997;88:355–365.
3. Hanahan D, Weinberg RA. The hallmarks of cancer. Cell 2000;100:57–70.
4. Liu X, Kim CN, Yang J, Jemmerson R, Wang X. Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell 1996;86:147–157.
5. Soung YH, Lee JW, Kim HS, et al. Somatic mutations of CASP3 gene in human cancers. Hum Genet 2004;115:112–115.
6. Lee SH, Shin MS, Park WS, et al. Alterations of Fas (Apo-1/CD95) gene in non-small cell lung cancer. Oncogene 1999;18:3754–3760.
7. Lee SH, Shin MS, Kim HS, et al. Alterations of the DR5/TRAIL receptor 2 gene in non-small cell lung cancers. Cancer Res 1999;59:5683–5686.
8. Shin MS, Kim HS, Lee SH, et al. Alterations of Fas-pathway genes associated with nodal metastasis in non-small cell lung cancer. Oncogene 2002;21:4129–4136.
9. Lee JW, Soung YH, Nam SW, Lee JY, Yoo NJ, Lee SH. Mutational analysis of pro-apoptotic BAD gene in non-small cell lung cancer. J Lung Cancer 2006;5:35–38.
10. Soung YH, Lee UW, Moon SW, et al. Mutational analysis of caspase-7 and 8 genes in non-small cell lung cancer. J Lung Cancer 2005;4:38–41.
11. Soung YH, Lee JW, Kim SY, et al. Mutational analysis of pro-apoptotic caspase-9 gene in common human carcinomas. APMIS 2006;114:292–297.
12. Reed JC. Apoptosis-targeted therapies for cancer. Cancer Cell 2003;3:17–22.
TOOLS
Similar articles

Mutational Analysis of Pro-apoptotic BNIP3 Gene in Non-Small Cell Lung Cancers

Mutational Analysis of Pro-apoptotic BAD Gene in Non-small Cell Lung Cancer

Mutational Analysis of PUMA Gene in Non-small Cell Lung Cancers

Mutational and Expressional Analysis of ATG5 Gene in Non-Small Cell Lung Cancers

Mutational and Expressional Analysis of DOK2 Gene in Non-small Cell Lung Cancers