Pro-apoptotic Cytochrome c Gene Mutation is Rare in Non-small Cell Lung Cancers

Youna Hwa Sounq; Sug Hyung Lee

doi:10.6058/jlc.2006.5.2.111

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Sounq and Lee: Pro-apoptotic Cytochrome c Gene Mutation is Rare in Non-small Cell Lung Cancers

Original Article

J Lung Cancer 2006;5(2):111-113.

Published online: 18 December 2006

DOI: https://doi.org/10.6058/jlc.2006.5.2.111

Pro-apoptotic Cytochrome c Gene Mutation is Rare in Non-small Cell Lung Cancers

Youna Hwa Sounq, Ph.D., Sug Hyung Lee, M.D.

¹Department of Pathology, Colleqe of Medicine, The Catholic University of Korea, Seoul, Korea

Address for correspondence Sua Hyuna Lee, MD. Department of Pathology, Colleqe of Medicine. The Catholic University of Korea, 505, BanDO-dong, Seochou, Seoul 137-701. Korea Tel: 82-2-590-1188 Fax: 82-2-537-6586 E-mail: suhulee@catholic.aakr

This work was supported bv the funds from KOSEF (R01-200MX)0-10463-0).

Received 3 December 2006 Accepted 11 December 2006

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose:

Several lines of evidence have indicated that the deregulation of apoptosis is involved in the mechanisms of cancer development, and somatic mutations of the apoptosis-related genes have been reported in human canGers. In addition to its role in oxidative phosphorylation, release of cytochrome c from the mitochondrial intermembrane space results in nuclear apoptosis. The aim of this study was to explore whether alteration of cytochrome c gene mutation is a characteristic of human non-small cell lung cancers (NSCLC).

Materials and Methods:

In the current study, to detect the somatic mutations in the DNA sequences encoding cytochrome c in 48 NSCLCs, we used polymerase chain reaction (PCR), single-strand conformation polymorphism (SSCP), and DNA sequencing.

Results:

The SSCP analysis revealed no mutation in the entire coding regions and all splice sites of human cytochrome c gene in the 48 NSCLCs.

Conclusion:

The data presented here indicate that the pro-apoptotic cytochrome c may not be somatically mutated in human NSCLCs, and suggest that NSCLCs may not utilize mutational events of cytochrome c gerie in the mechanisms for evading apoptosis. (J Lung Cancer 2006;5(2):111 -113)

Keywords: Non-small cell lung cancer, Cytochrome c, Apoptosis, Mutation

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Fig. 1.

Representative SSCP of cytochrome c gene in the non-small cell lung cancers. The exon 2 of the cytochrome c gene was amplified by PCR using a specific primer set. The PCR products from the representative cases of non-small cell lung cancers were visualized on SSCP. SSCP of DNA from the non-small cell lung cancers (T) shows no aberrant bands as compared to SSCPs from the normal tissues (N).

Table 1.

Sequences of PCR Primer Sets for Cytochrome c Gene Amplification

Pimers	Exon	Sequence	Annealing Tm (°C)	Size (bp)
1A	1	F 5’-GTTGAAGCTTTCGTTTTTAG-3’	58	172
		R 5’-GQCTQTQTAAGAGTATCCAG-3’
1B	1	F 5-GCCACACCGTTGAAAAG-3’	66	195
		R 5’-CTCCTGATAGTTTGCCACAT-3’
2A	2	F 5’-GCAAACTATCAGQAGTGTQ-3’	56	187
		R 5’-ATTAAGTCTGCCCTTTCnC-3’
2B	2	F 5’-GTTTAGGCATCATCTGG-3’	56	187
		R 5’-TGTAATAAATAAQGCAQTQG 3’

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