Effect of rhBMP-2 produced by Escherichia coli expression system on bone formation in rat calvarial defects

Suk-Hoon Kwon; Hyun-Chang Lim; Kyung-Hee Choi; Min-Soo Kim; Ji-Hyun Lee; Ui-Won Jung; Jeong-Ho Yun; Chang-Sung Kim; Seong-Ho Choi; Kyoo-Sung Cho

doi:10.5051/jkape.2009.39.1.77

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Kwon, Lim, Choi, Kim, Lee, Jung, Yun, Kim, Choi, and Cho: Effect of rhBMP-2 produced by Escherichia coli expression system on bone formation in rat calvarial defects

Original Article

The Journal of the Korean Academy of Periodontology

The Journal of the Korean Academy of Periodontology 2009; 39(1): 77-86.

Published online: 31 March 2009

DOI: https://doi.org/10.5051/jkape.2009.39.1.77

Effect of rhBMP-2 produced by Escherichia coli expression system on bone formation in rat calvarial defects

Suk-Hoon Kwon¹, Hyun-Chang Lim¹, Kyung-Hee Choi², Min-Soo Kim¹, Ji-Hyun Lee¹, Ui-Won Jung¹, Jeong-Ho Yun³, Chang-Sung Kim¹, Seong-Ho Choi¹, Kyoo-Sung Cho¹

¹Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University, Seoul, Korea.

²Research Development Institute, Cowellmedi Co. LTD, Korea.

³Department of Dentistry, College of Medicine, Kwandong University, Myongji Hospital, Goyang, Korea.

Correspondence: Kyoo-Sung Cho. Department of Periodontology, School of Dentistry, Yonsei University, 134 Shin-chon Dong, Seodaemoon-gu, Seoul 120-752, Korea. kscho@yuhs.ac, Tel: 02-2228-3188, Fax: 02-392-0398

Received 12 February 2009 Accepted 11 March 2009

Abstract

Purpose

Recombinant human bone morphogenetic protein-2(rhBMP-2) has been evaluated as potential candidates for periodontal and bone regenerative therapy. In spite of good prospects in BMP applications, there is economically unavailable for clinical use in dental area. The purpose of this study was to evaluate the osteogenic effect of rhBMP-2 produced by E.coli expression system.

Materials and methods

Eight-mm critical-size calvarial defects were created in 48 male Sprague-Dawley rats. The animals were divided into 6 groups of 8 animals each. Each group received one of the following: Negative control(sham-surgery control), positive control(absorbable collagen sponge(ACS) alone) and experimental(ACS loaded with rhBMP-2). Defects were evaluated by histologic and histometric parameters following 2- and 8-week healing intervals.

Results

The experimental group showed significant defect closure at 2 and 8weeks than the sham surgery and positive control groups. Moreover, the experimental group showed significantly greater new bone and augmented area than the other groups at both 2 and 8weeks.

Conclusion

rhBMP-2 produced by E.coli expression system may be effective for bone regeneration.

Keywords: bone morphogenetic protein-2, E.coli expression system, Absorbable collagen sponge, rat calvarial defect

Figures and Tables

Figure 1

Schematic diagram of the calvarial osteotomy defect showing the histometric analysis

Figure 2

Representative photomicrographs of defect site receiving the sham surgery at 2weeks(arrow heads : defect margin ; HE stain, magnification × 10).

Figure 3

Representative photomicrographs of defect site receiving the sham surgery at 8weeks(arrow heads : defect margin ; HE stain, magnification × 10).

Figure 4

Representative photomicrographs of defect site receiving the positive control at 2weeks(arrow heads : defect margin ; HE stain, magnification × 10).

Figure 5

Representative photomicrographs of defect site receiving the positive control at 8weeks(arrow heads : defect margin ; HE stain, magnification × 10).

Figure 6

Representative photomicrographs of defect site receiving the experimental at 2weeks(arrow heads:defect margin ; HE stain, magnification × 10).

Figure 7

Representative photomicrographs of defect site receiving the experimental at 2weeks(arrow heads:defect margin, PB:pre-existing bone, NB:new bone ; HE stain, magnification × 100).

Figure 8

Representative photomicrographs of defect site receiving the experimental at 2weeks(central portion ; HE stain, magnification × 100)

Figure 9

Representative photomicrographs of defect site receiving the experimental at 8weeks(arrow heads: defect margin ; HE stain, magnification × 10).

Figure 10

Representative photomicrographs of defect site receiving the experimental at 8weeks(arrow heads:defect margin, PB:pre-existing bone, NB:new bone ; HE stain, magnification × 100).

Figure 11

Representative photomicrographs of defect site receiving the experimental at 8weeks(central portin; HE stain, magnification × 100).

Table 1

Study design

Table 2

Defect closure(group means±SD; n=8, %)

^*: Statistically significant difference compared with the negative control group (P<0.05)

^¶: Statistically significant difference compared with the positive control group (P<0.05)

^∮: Statistically significant difference compared with that observed at 2 weeks (P<0.05)

Table 3

New bone area(group means±SD; n=8, mm²)

^*: Statistically significant difference compared with the negative control group (P<0.05)

^¶: Statistically significant difference compared with the positive control group (P<0.05)

^∮: Statistically significant difference compared with that observed at 2 weeks (P<0.05)

Table 4

Augmented area(group means±SD; n=8, mm²)

^*: Statistically significant difference compared with the negative control group (P<0.05)

^¶: Statistically significant difference compared with the positive control group (P<0.05)

^∮: Statistically significant difference compared with that observed at 2 weeks (P<0.05)

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