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Ju-A Lee1, Young Ku1, Yoon-Jeong Park2, Ki-Tae Koo1, Tae-Il Kim1
, Yang-Jo Seol1, Yong-Moo Lee1, In-Chul Rhyu1, Chong-Pyoung Chung1
, Yang-Jo Seol1, Yong-Moo Lee1, In-Chul Rhyu1, Chong-Pyoung Chung1
Abstract
Purpose
Fibronectin(FN), one of the major components of ECM, mediates wide variety of cellular interactions including cell adhesion, migration, proliferation and differentiation. In this study, we used synthetic peptides based on fibrin binding sites of amino-terminal of FN and evaluated their biologic effects on periodontal ligament(PDL) cells.
Materials and methods
PDL cells were cultured on synthetic oligopeptides coated dishes and examined for cell adhesion, proliferation via confocal microscope. For detection of ERK1/2, cells were plated and Western blot analysis was performed.
Figures and Tables
Figure 1
PDL cell attachment using confocal laser microscopy. Nuclei appear blue, and actin filaments are red. (A) NT, (B) FF1, (C) FF3, (D) FF5
Figure 2
MTT assay of PDL cell attachment in 1 hour. *denotes a statistically significant differences between control and test group (P<0.05). †denotes statistically significant differences between test groups(P<0.05).
Figure 3
Activation of ERK1/2 in PDL cells cultured on synthetic oligopeptide. Phosphorylation levels of p-ERK1/2 were quantified by densitometry. Immunoblotting after treatment with 20µM of each peptide. (A) Phosphorylated ERK1/2, (B) β-actin, (C) The levels of p-ERK1/2 were quantified by densitometric scanning
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